Bioelectricity Production and Comparative Evaluation of Electrode Materials in Microbial Fuel Cells Using Indigenous Anode-Reducing Bacterial Community from Wastewater of Rice-Based Industries

DOI: https://doi.org/10.14710/ijred.6.1.83-92

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Submitted: 13-12-2016
Published: 22-03-2017
Section: Articles
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Microbial fuel cells (MFCs) are the electrochemical systems that harness the electricity production capacity of certain microbes from the reduction of biodegradable compounds. The present study aimed to develop mediator-less MFC without using expensive proton exchange membrane. In the present study, a triplicate of dual-chamber, mediator-less MFCs was operated with two local rice based industrial wastewater to explore the potential of this wastewater as a fuel option in these electrochemical systems. 30 combinations of 6 electrodes viz. Carbon (14 cm × 1.5 cm), Zn (14.9 cm × 4.9 cm), Cu (14.9 cm × 4.9 cm), Sn (14.1cm × 4.5cm), Fe (14cm × 4cm) and Al (14cm × 4.5 cm) were evaluated for each of the wastewater samples. Zn-C as anode-cathode combination produced a maximum voltage that was 1.084±0.016V and 1.086±0.028 and current of 1.777±0.115mA and 1.503±0.120 for KRM and SSR, respectively. In the present study, thick biofilm has been observed growing in MFC anode. Total 14 bacterial isolates growing in anode were obtained from two of the wastewater. The dual chambered, membrane-less and mediator-less MFCs were employed successfully to improve the economic feasibility of these electrochemical systems to generate bioelectricity and wastewater treatment simultaneously.

Keywords: Membrane-less, Microbial Fuel Cells, Biofilm, Wastewater, Electrogenic.

Article History: Received June 25th 2016; Received in revised form Dec 15th 2016; Accepted January 5th 2017; Available online

How to Cite This Article: Reena, M. and Jadhav, S. K. (2017) Bioelectricity production and Comparative Evaluation of Electrode Materials in Microbial Fuel Cells using Indigenous Anode-reducing Bacterial Community from Wastewater of Rice-based Industries. International Journal of Renewable Energy Develeopment, 6(1), 83-92.

http://dx.doi.org/10.14710/ijred.6.1.83-92

 

 

Keywords

Membrane-less; Microbial Fuel Cells; Biofilm; Wastewater; Electrogenic.

  1. Shailesh Kumar Jadhav 
    School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh - 492010, India, India

    Professor & Head,

    School of Studies in Biotechnology,

    Pt. Ravishankar Shukla University, Raipur,

    Chhattisgarh - 492010, India

  2. Reena Meshram 
    School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh - 492010, India, India

    Research Scholar,

    School of Studies in Biotechnology,

    Pt. Ravishankar Shukla University, Raipur,

    Chhattisgarh - 492010, India

  1. Ahn, Y. & Logan, B. E. (2010) Effectiveness of domestic wastewater treatment using microbial fuel cells at ambient and mesophilic temperatures. Bioresource Technology, 101, 469-475.
  2. Amade, R., Vila-Costa, M., Hussain, S., Casamayor, O. E. & Bertran, E. (2015) Vertically aligned carbon nanotubes coated with manganese dioxide as cathode material for microbial fuel cells. Journal of Materials Science 50, 1214-1220.
  3. APHA (1998) Standard Methods for the Examination of Water and Wastewater. American Public Health Association, American Water Works Association, Water Environment Federation, Washington, DC.
  4. Baranitharan, E., Khan, M. R., Prasad, D. M. R. & Salihon, J. B. (2013) Bioelectricity generation from palm oil mill effluent in microbial fuel cell using polacrylonitrile carbon felt as electrode. Water Air Soil Pollution, 224, 1533-1544.
  5. Behera, M., Jana, P. S., More, T. T. & Ghangrekar, M. M. (2010) Rice mill wastewater treatment in microbial fuel cells fabricated using proton exchange membrane and earthen pot at different pH. Bioelectrochemistry, 79, 228-233.
  6. Bond, D. R. & Lovley, D. R. (2003) Electricity production by Geobacter sulfurreducens attached to electrodes. Applied and Environment Microbiology, 69, 1548- 1555.
  7. Brown, M. A. (2001) Market failures and barriers as a basis for clean energy policies. Energy Policy, 29, 1197–1207.
  8. Buitrón, G. & Cervantes-Astorga, C. (2013) Performance Evaluation of a Low-Cost Microbial Fuel Cell Using Municipal Wastewater. Water Air Soil Pollution, 224, 1470-1478.
  9. Daniel, D. K., Mankidy, B. D., Ambarish, K. & Manogari, R. (2009) Construction and operation of a microbial fuel cell for electricity generation from wastewater. International Journal of Hydrogen Energy, 34, 7555-7560.
  10. Elakkiya, E. & Matheswaran, M. (2013) Comparison of anodic metabolisms in bioelectricity production during treatment of dairy wastewater in Microbial Fuel Cell. Bioresource Technology, 136, 407-412.
  11. Feng, Y., Wang, X., Logan, B. E. & Lee, H. (2008) Brewery wastewater treatment using air-cathode microbial fuel cells. Applied Microbiology and Biotechnology, 78, 873-880.
  12. Gil, G-C., Chang, I-S., Kim, B. H., Kim, M., Jang, J-K., Park, H. S. & Kim, H. J. (2003) Operational parameters affecting the performance of a mediator-less microbial fuel cell. Biosens Bioelectron, 18, 327- 334.
  13. Goud, R. K. & Mohan, S. V. (2011) Pre-fermentation of waste as a strategy to enhance the performance of single chambered microbial fuel cell (MFC). International Journal of Hydrogen Energy, 36, 13753-13762.
  14. Gregory, K. B., Bond, D. R. & Lovley, D. R. (2004) Graphite electrodes as electron donors for anaerobic respiration, Environment Microbiology, 6 596- 604.
  15. http://descg.gov.in/pdf/publications/latest/ECONOMICSURVEY13-14.pdf. Retrieved on 26-12-2014.
  16. Huang, L. & Logan, B. E. (2008) Electricity generation and treatment of paper recycling wastewater using a microbial fuel cell. Applied Microbiology and Biotechnology, 80, 349- 355.
  17. Ishii, S., Watanabe, K., Yabuki, S., Logan, B. E. & Sekiguchi, Y. (2008) Characterization of electrode reducing rates of Geobacter sulfurreducens and an enriched electricity-generating mixed consortium in a microbial fuel cell. Appl. Environ. Microbiol. 74, 7348–7355.
  18. Jayashree, C., Arulazhagan, P., Kumar, S. A., Kaliappan, S., Yeom, I. T. & Banu, R. J. (2014) Bioelectricity generation from coconut husk retting wastewater in fed batch operating microbial fuel cell by phenol degrading microorganism. Biomass Bioenerg, 69, 249-254.
  19. Kalathil, S., Lee, J. & Cho, M. H. (2012) Efficient decolorization of real dye wastewater and bioelectricity generation using a novel single chamber biocathode-microbial fuel cell. Bioresource Technology, 119, 22-27.
  20. Kim, B-H., Kim, H., Hyun, M. & Park, D. (1999) Direct electrode reduction of fe(III)-reducing bacterium Shewanella putrefaciens. J Microbial Biotechnol, 9, 127-131.
  21. Kim, J. E., Dec, J., Bruns, M. E. & Logan, B. E. (2008) Reduction of Odors from Swine Wastewater by Using Microbial Fuel Cells. Appl Environ Microbiol, 74(8), 2540- 2543.
  22. Kumar, S., Kumar, H. D. & Babu, G. K. (2012) A study on the electricity generation from the cow dung using microbial fuel cell. J Biochem Tech, 3, 442- 447.
  23. Liu, H., Ramnarayanan, R. & Logan, B. E. (2004) Production of electricity during wastewater Treatment using a single chamber microbial fuel cell. Environ Sci Technol, 38, 2281- 2285.
  24. Logan, B. E. & Regan, J. M. (2006) Microbial fuel cells– challenges and applications. Environ Sci Technol, 40(17), 5172- 5180.
  25. Logan, B. E. (2005) Simultaneous wastewater treatment and biological electricity generation. Water Sci Technol, 52, 31- 37.
  26. Logan B. E. (2009) Exoelectrogenic bacteria that power microbial fuel cells. Nature Reviews | Microbiology, 7, 375-381.
  27. Logan, B. E., Call, D., Cheng, S., Hamelers, H. V. M., Sleutels, T. H. J. A., Jeremiasse, A. W. & Rozendal, R. A. (2008) Microbial electrolysis cells for high yield hydrogen gas production from organic matter. Environ Sci Technol, 42, 8630- 8640.
  28. Lovely, D. R., Giovannoni, S. J., White, D. C., Champine, J. E., Phillips, E. J., Gorhy, Y. A. & Goodwin, S. (1993) Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals. Arch Microbial, 159, 336 – 344.
  29. Lu, N., Zhou, S., Zhuang, L., Zhang, J. & Ni, J. (2009) Electricity generation from starch processing wastewater using microbial fuel cell technology. Biochem Eng J, 43, 246- 251.
  30. Luo, H., Liu, G., Zhang, R. & Jin, S. (2009) Phenol degradation in microbial fuel cells. Chem Eng J, 147, 259- 264.
  31. Majumder, D., Maity, J. P., Tseng, M., Nimje, V. R., Chen, H., Chen, C., Chang, Y., Yang, T. & Chen, C., (2014) Electricity generation and wastewater treatment of oil refinery in microbial fuel cells using Pseudomonas putida. Int J Mol Sci, 15, 16772-16786.
  32. Mohanakrishna, G., Mohan, S. V. & Sarma, P. N., (2010) Bioelectrochemical treatment of distillery wastewater in microbial fuel cell facilitating decolorization and desalination along with power generation. J Hazard Mater, 177, 487-494.
  33. Momoh, O. L. & Naeyor, B. A. (2010) A novel electron acceptor for microbial fuel cells: Nature of circuit connection on internal resistance. J Biochem Tech, 2, 216- 220.
  34. Pangare, G., Pangare, V. & Das, B. (2006) Springs of Life: India's Water Resource, Academic foundation, World Water Institute, New Delhi India.
  35. Park, H. S., Kim, B. H., Kim, H. S., Kim, H. J., Kim, G. T., Kim, M., Chang, I-S., Park, Y. K. & Chang, H. I. (2001) A novel electrochemically active and Fe(III)-reducing bacterium Phylogenetically Related to Clostridium butyricum Isolated from a microbial fuel cell. Anaerobe, 7, 297-306.
  36. Patil, S. A., Surakasi, V. P., Koul, S., Ijmulwar, S., Vivek, A., Shouche, Y. S. & Kapadnis, B. P. (2009) Electricity generation using chocolate industry wastewater and its treatment in activated sludge based microbial fuel cell and analysis of developed microbial community in the anode chamber. Bioresour Technol, 100, 5132-5139.
  37. Qu, Y., Feng, Y., Wang, X., Logan B. E. (2012) Use of a Coculture To Enable Current Production by Geobacter sulfurreducens. Applied and Environmental Microbiology, 78 (9), 3487- 3487.
  38. Rabaey, K. & Verstraete, W. (2005) Microbial fuel cells: novel biotechnology for energy generation. Trends Biotechnol, 23, 291–298.
  39. Singh, S. & Songera, D. S. (2012) A review on microbial fuel cell using organic waste as feed. CIBTech Journal of Biotechnol, 2, 17- 27.
  40. Vignesh, H. & Rani, K. (2012) Generation of Bioelectricity from Waste water and Cow’ urine. Indian J of App res, 1, 16-19.
  41. Wang, X., Cheng, S., Feng, Y., Merrill, M. D., Saito, T. & Logan, B. E. (2009) Use of carbon mesh anodes and the effect of different pre-treatment methods on power production in microbial fuel cells. Environ Sci Technol, 43, 6870 -6874.
  42. Water for People, Water for Life - UN World Water Development Report (WWDR) March 2003, Retrieved on 12-11-2014.
  43. Wei, M., Patadia, S. & Kammen, D. M. (2010) Putting renewable and energy efficiency to work: How many jobs can the clean energy industry generate in the US? Energy Policy, 38, 919–931.
  44. Yi, H., Nevin, K. P., Kim, B., Franks, A. E., Klimes, A., Tender, M. L. & Lovley, D. R. (2009) Selection of a variant of Geobacter sulfurreducens with enhanced capacity for current production in microbial fuel cells. Biosens Bioelectron, 24, 3498-3503.
  45. Zhang, L., Zhou, S., Zhuang, L., Li, W., Zhang J., Lu N., Deng, L. (2008) Microbial fuel cell based on Klebsiella pneumoniae biofilm. Electrochemistry Communications, 10, 1641–1643.