skip to main content

Pengaruh Garam Monovalen (NaCl dan KCl) dan Divalen (CaCl2 dan MgCl2) Terhadap Aktivitas Protease Ekstraseluler Bakteri Halofilik Isolat Bittern Tambak Garam Madura

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Apr 2017.
Open Access Copyright 2017 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0/.

Citation Format:
Abstract
Bakteri halofilik mampu tumbuh dan bertahan hidup pada lingkungan dengan kadar garam tinggi, seperti dalam air pekatan sisa tambak garam (bittern). Bakteri halofilik dapat menghasilkan enzim protease yang tahan terhadap garam (halostabil). Protease halostabil dapat dimanfaatkan pada proses degradasi limbah pada kawasan yang mengandung garam. Penelitian ini bertujuan untuk memperoleh protease ekstraseluler bakteri halofilik isolat bittern tambak garam Madura dan menentukan pengaruh garam monovalen (NaCl dan KCl) dan divalen (CaCl2 dan MgCl2) terhadap aktivitas dan profil aktivitas protease halostabil. Berdasarkan penelitian diperoleh protease halostabil memiliki aktivitas spesifik tertinggi pada fraksi 1 (0-20%) sebesar 220,83 Unit/mg protein. Pengaruh garam NaCl, CaCl2 dan MgCl2 akan cenderung meningkatkan aktivitas proteasenya sebaliknya KCl akan menurunkan aktivitas protease. Profil pengaruh penambahan garam terhadap aktivitas protease semakin meningkat sesuai dengan urutan KCl. NaCl, CaCl2, MgCl2.
Fulltext View|Download
Keywords: bakteri halofilik; protease halostabil; bittern; garam monovalen; divalen

Article Metrics:

  1. Aharon Oren, Halophilic Microorganisms and their Environments, Springer Science & Business Media, 2002
  2. Shiladitya DasSarma, Priya DasSarma, Halophiles, in: Encyclopedia of Life Science, Wiley, 2006
  3. Mital S. Dodia, Rupal H. Joshi, Rajesh K. Patel, Satya P. Singh, Characterization and stability of extracellular alkaline proteases from halophilic and alkaliphilic bacteria isolated from saline habitat of coastal Gujarat, India, Brazilian Journal of Microbiology, 37, (2006) 276-282 http://dx.doi.org/10.1590/S1517-83822006000300015
  4. Neil D. Rawlings, Protease Families, Evolution and Mechanism of Action, in: K. Brix, W. Stöcker (Eds.) Proteases: Structure and Function, Springer Vienna, Vienna, 2013, pp. 1-36
  5. P. Norberg, B. V. Hofsten, Proteolytic Enzymes from Extremely Halophilic Bacteria, Microbiology, 55, 2, (1969) 251-256 http://dx.doi.org/10.1099/00221287-55-2-251
  6. Janos K. Lanyi, Salt-dependent properties of proteins from extremely halophilic bacteria, Bacteriological Reviews, 38, 3, (1974) 272-290
  7. Janos K. Lanyi, Joann Stevenson, Studies of the Electron Transport Chain of Extremely Halophilic Bacteria: IV. Role of Hydrophobic Forces in The Structure of Menadione Reductase, Journal of Biological Chemistry, 245, 16, (1970) 4074-4080
  8. Malashetty Vidyasagar, S Prakash, Carol Litchfield, K Sreeramulu, Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeon Halogeometricum borinquense strain TSS101, Archaea, 2, 1, (2006) 51-57
  9. Ronald M. Atlas, Richard Bartha, Microbial Ecology: Fundamentals and Applications, 3 ed., Benjamin/Cummings Publishing Company, 1993
  10. John P. Harley, Lansing M. Prescott, Laboratory Exercises in Microbiology, 5 ed., McGraw-Hill, 2001
  11. Werasit Kanlayakrit, Preeyanuch Bovornreungroj, Takuji Oka, Masatoshi Goto, Production and characterization of protease from an extremely halophilic Halobacterium sp. PB407, Kasetsart Journal: Natural Science, 38, 5, (2004) 15-20
  12. J. Charney, R. M. Tomarelli, A colonmetric method for the determination of the proteolytic activity of duodenal juice, Journal of Biological Chemistry, 171, (1947) 501-505
  13. Terry J. Beveridge, Structures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles, Journal of Bacteriology, 181, 16, (1999) 4725-4733
  14. R. McClelland, Gram's stain: the key to microbiology, MLO Med Lab Obs, 33, 4, (2001) 20-22, 25-28; quiz 30-21
  15. Clive Dennison, A Guide to Protein Isolation, Kluwer Academic Publishers, 1999
  16. Tim Bugg, Introduction to Enzyme and Coenzyme Chemistry, John Wiley & Sons, 2004

Last update:

  1. Keratinase from Salinivibrio proteolyticus TGS

    Sahda Uma Khanifah, Nailar Rokhmah, Nur Faridah, Suharti Suharti. THE 4TH INTERNATIONAL CONFERENCE ON LIFE SCIENCE AND TECHNOLOGY (ICoLiST), 2634 , 2023. doi: 10.1063/5.0116143

Last update: 2024-03-28 00:41:40

No citation recorded.