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Studi Kinetika Hidrolisis Tandan Kosong Kelapa Sawit (TKKS) Dengan Proses Fermentasi Padat Menggunakan Jamur Aspergillus niger

*Fitri Nur Kayati  -  Jurusan Teknik Kimia, Fakultas Teknik, Universitas Gadjah Mada, Indonesia
Siti Syamsiah  -  Jurusan Teknik Kimia, Fakultas Teknik, Universitas Gadjah Mada, Indonesia
Wahyudi Budi Sediawan  -  Jurusan Teknik Kimia, Fakultas Teknik, Universitas Gadjah Mada, Indonesia
Sutijan Sutijan  -  Jurusan Teknik Kimia, Fakultas Teknik, Universitas Gadjah Mada, Indonesia

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Abstract

Kinetic of hydrolysis of Oil Palm Empty Fruit Bunch (OPEFB) by Solid State Fermentation using Aspergillus niger is studied. Hydrolysis of OPEFB to simple sugars using Aspergillus niger as raw material for other products is a potential alternative of bioconversion of lignocellulose. Formulating the kinetics of hydrolysis during solid substrate cultivication is useful for effective scale up of this technology as well as for better understanding of the process. The aim of this study was to develop kinetics models to describe the solid state fermentation of OPEFB. First, OPEFB were ground and classified by size using screen, with size range of : -30+40 mesh, -40+80 mesh and -80 mesh. It turned out that the proposed based on first order reaction kinetics model can quantitatively describe the process reasionally well. Furthermore, the values of the parameters involved in the kinetics models for each size for OPEFB particle were also obtained. The smaller the particle, the larger the hydrolysis reaction rate constant would be. It means that the rate of hydrolysis increases by the reduction of the particle size. Meanwhile, the sugars consumption rate constant decreases also by the reduction of OPEFB particle size. The highest concentration of simple sugars produced in this experiment was 7,847 g/L.

Kata kunci : kinetic, Aspergillus niger,solid state fermentation, OPEFB, particle size

Hidrolisis tandan kosong kelapa sawit (TKKS) menjadi gula sederhana dengan menggunakan jamur Aspergillus niger merupakan alternatif biokonversi lignoselulosa yang potensial. Tujuan penelitian ini adalah mengembangkan model kinetika untuk mendiskripsikan proses fermentasi padat dengan bahan baku TKKS. Tahap pertama, TKKS digiling dan dikelompokkan sesuai ukuran dengan menggunakan ayakan,  dengan variasi ukuran antara -30+40 mesh, -40+80 mesh dan -80 mesh. Model kinetika yang telah disusun didasarkan pada reaksi orde satu. Model tersebut dapat menggambarkan secara kuantitatif proses fermentasi tersebut. Selain itu, nilai – nilai parameter dalam kinetika reaksi untuk setiap ukuran partikel juga akan diperoleh dari model tersebut. Semakin kecil ukuran partikel, semakin besar pula konstanta kecepatan reaksi hidrolisis. Sebaliknya konstanta konsumsi gula menurun seiring dengan penurunan ukuran partikel TKKS. Konsentrasi gula sederhana tertinggi yang dihasilkan dalam penelitian ini adalah sebesar 7,847% g/L.

 

Kata kunci: kinetika, Aspergillus niger, fermentasi padat, TKKS, ukuran partikel


 

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Keywords: kinetic, Aspergillus niger,solid state fermentation, OPEFB, particle size

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  1. Abd-Aziz, S., Hung, G.S., Hassan, M.A., Abdul Karim, M.I., and Samad, N., 2008, Indirect method for quantification of cell biomass during solid state fermentation of palm kernel cake based on protein content, Asian Journal of Scientific Research, 1(4), pp. 385-393
  2. Yeh, A-I, Yi-Ching, H., and Chen, S.H., 2010, Effect of particle size on the rate of enzymatic hydrolysis of cellulose, Carbohydrate Polymers, 79, pp. 192-199
  3. Barrington, S., and Kim, J.W., 2008, Response surface optimization of medium components for citric acid production by Aspergillus niger NRRL 567 grown in peat moss, Bioresource Technology, 99, pp. 368–377
  4. Dasari, R.K., & Berson, R. E., 2007, The effect of particle size on hydrolysis reaction rates and rheological properties in cellulosic slurries, Applied Biochemistry and Biotechnology, pp. 289-300
  5. Datta, R., 1981, Acidogenic fermentation of lignocellulose-acid yield and conversion of components, Biotechnology and Bioengineering, 23 (9), pp. 2167-2170
  6. Dawson, L., and Boopathy, R., 2008, Cellulosic ethanol production from sugarcane baggase without enzymatic saccharification, BioResources, 3, pp. 452-460
  7. Departemen Pertanian RI, 2014, http://ditjenbun.deptan.go.id/cigraph/index.php/viewstat/ komoditiutama/8-Kelapa%20Sawit, diakses pada tanggal 24 Agustus 2014
  8. Gan, Q., Allen, S.I., and Taylor, G., 2003, Kinetic dynamics in heterogeneous enzymatic hydrolysis of cellulose : An overview, an experimental study and mathematical modelling, Process Biochemistry, 38, pp. 1003-1018
  9. Johnston, D.B., Shoemaker, S.P., Smith, G.M., and Whitaker, J.R., 1998, Kinetic measurements of cellulase activity on insoluble substrates using disodium 2, 2’ bicinchoninate, Journal of Food Biochemistry, 22, pp. 301-319
  10. Krishna, C., 2005, Solid state fermentation systems-an overview, Crit.Rev. in Biotechnol., 25(1-2), pp.1-30
  11. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., 1951, Protein measurement with the folin phenol reagent, J Biol. Chem., 193, pp. 265-275
  12. Miller, G.L., 1959, Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem., 31, pp. 426-428
  13. Nigam, P.S. and Pandey, A., 2009, Solid-state fermentation technology for bioconversion of biomass and agricultural residues, In: Biotechnology for agro-industrial residues utilisation, Edited by: Nigam, P.S. and Pandey, A. Springer Science + Business Media B.V., pp. 197 – 221
  14. Shuler M.L., and Kargi F., 2002. Bioprocess engineering basic concepts, 2nd ed., Prentice-Hall International Inc., New Jersey
  15. Sulhatun, 2005, Pemanfaatan tandan kosong kelapa sawit sebagai sumber lignin, Tesis Program Studi Magister Teknik Kimia Universitas Sumatera Utara, Sumatera Utara, Indonesia
  16. Umi Kalsom, M. S., Arbakariya, A., and Karim, M. I. A., 1998, Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of chaetomium globosum, Journal of Agricultural and Food
  17. Widiyanti, S. E., 2014, Kinetika konsumsi glukosa oleh aspergillus niger dalam produksi bioethanol dari lignoselulosa, Tesis Jurusan Teknik Kimia Fakultas Teknik Universitas Gadjah Mada, Yogyakarta, Indonesia

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