Zinc Supplementation Dosage Variations to Metallothionein Protein Level of Rattus Norvegicus

Budi Santosa, Hertanto Wahyu Subagyo, Lisyani S, Henna Rya Sunoko

DOI: https://doi.org/10.12777/ijse.5.2.15-17


Zinc is an essential trace element involving in the activity of more than 300 enzymes and proteins of human body. One important role of zinc is to improve metallothionein protein binding heavy metals and functioning as heavy metal detoxification facilities. This research discusses the effect of zinc supplement  on the  improvement of metallothionein protein level. The in vivo test involving 28 rats categorized in 4 groups was performed. The experiments used randomized post test control group design. The 3 groups were daily supplemented by zinc in th concentration of  0.2 mg, 0.4 mg, and 0.8 mg. Whereas, the last group was let without zinc treatment. As an indicator the metallothionein protein level was checked after three weeks. The data was then evaluated by Anova an Bonferroni test in order to know the signicant of protein level difference among the groups.

The result showed that the average of metallothionein protein level improved by increasing zinc suppplement with the 0.95 ±0.20; 1.28 ±0.19; 1.39 ±0.09; 1.91 ±0.3 ng/ml metallothionein per 0, 0,2, 0,4, 0,8.mg zinc added. Based on the ANOVA and Bonferroni test,  indicated that the improvement was significant as shown with p value  of 0.00


Zinc supplementation, metallothionein

Full Text:



Allen,L.H., (1998). Zinc and Micronutrient Suplement for Children. Am J Clin Nutr.;68:495S-8S

Aydemir, T.B., Raymond, K.B., Cousins, R.J., (2005). Zinc supplementation of young men alters metallothionein, zinc transporter, and cytokine gene expression in leukocyte populations. Nutritional Genomics Laboratory, Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville;FL 32611-0370

Berdanier, C.D., (1998). Advanced nutricion micronutrients. New York: CRC Press.;183-203.

Blalock, T.L., Dunn,M.A., & Cousins, R. J. (1988). Metallothionein gene expression in rats: tissue-specific regulation by dietary copper and zinc. J. Nutr.;118: 222–8.

Brown, K.H., Peerson, J.m., Rivera, J., Allen, L.H., (2002). Effect of Suplementation zinc on the growth and serum zinc concentration of pre pubertal children: a meta analysis of randomized controlled trials. Am J Clin Nutr.;75:1062-71.

Cheung, R.C.K., Chan, M.H.M., Lam, C.W.K., and Lau, E.L.K., (2001). Heavy metal poisoning clinical significance and laboratory investigation.Asia pasific Analyte Notes. BD Indispensable to Human Health. Hong Kong.;7(1):22-34

Donatus, I.A., (1994). Petunjuk Praktikum Toksikologi. Edisi I. Yogyakarta: Lab. Farmakologi dan Toksikologi. Fakultas Farmasi Universitas Gadjah Mada.;21-2

Gallant, K.R., Cherian, G., (1987).Changes in Dietary Zinc Result in Specific Alterations of Metallothionein Concentrations in Newborn Rat Liver. The Journal of Nutrition.;117:709-16.

Jiang, S., Rui Guo., Zhang, Y., Zou, Y., and Ren, J., (2013). Heavy metal scavenger metallothionein mitigates deep hypothermia-induced myocardial contractile anomalies: role of autophagy.Am J Physiol Endocrinol Metab.; 304: 74 - 86.

Lee, S.M., McLaughlin, J.N., Frederick, D.R., Zhu, L., Thambiayya, K., Wasserloos, K.J., (2013). Metallothionein-induced zinc partitioning exacerbates hyperoxic acute lung injury. Am J of Physiology - Lung Cellular and Molecular Physiology.;304: 350-60.

Steven, R., Davis and Cousin, J.R., (2000). Metallothionein Expression in Animal: A Physiological Perspective on Function. American Society for Nutrition Sciences, Food Science and Human Nutrition Departement, University of Florida, Gainesville FL.; 32611-0370.

Sullivan, V.K., Burnett, F.R., and Cousins, R.J., (1998). Metallothionein Expression is Increased in Monocytes and Erythrocytes of Young Men during Zinc Suplementation. J. Nutr.;128:703-7

Villalobos, A.R., and Young, R.K., (2011). Metallothionein (MT-1) siRNA compromises the efficacy of Zn to ameliorate stress-modulation of choline transport in choroid plexus. FASEB J.; 25(3): 838.

Zlotkin, S.H., Cherian, M.G.,(1988). Hepatic metallothionein as a source of zinc and cysteine during the first year of life. Pediatr Res. ;24(3):326-9


  • There are currently no refbacks.

Published by Department of Chemical Engineering University of Diponegoro Semarang
Google Scholar

IJSE  by http://ejournal.undip.ac.id/index.php/ijse is licensed under Creative Commons Attribution 3.0 License.