Increasing Biogas Production Rate from Cattle Manure Using Rumen Fluid as Inoculums

Budiyono Budiyono, I Nyoman Widiasa, Seno Johari, S Sunarso



In this study, rumen fluid of animal ruminant was used as inoculums to increase biogas production rate from cattle manure at mesophilic condition. A series of laboratory experiments using 400 ml biodigester were performed in batch operation mode. Given 100 grams of fresh cattle manure (M) was fed to each biodigester and mixed with rumen fluid (R) and tap water (W) in several ratio resulting six different M:W:R ratio contents i.e. 1:1:0; 1:0.75:0.25; 1:0.5:0.5; 1:0.25:0.75; and 1:0:1 (correspond to 0; 12.5; 25, 37.5; 50, and 100 % rumen, respectively) and six different total solid (TS) contents i.e. 2.6, 4.6, 6.2, 7.4, 9.2, 12.3, and 18.4 %. The operating temperatures were at room temperature. The results showed that the rumen fluid inoculated to biodigester significantly effected the biogas production. Rumen fluid inoculums caused biogas production rate and efficiency increase more than two times in compare to manure substrate without rumen fluid inoculums. The best performance for biogas production was the digester with rumen fluid and TS content in the range of 25-50 % and 7.4 and 9.2 %, respectively. These results suggest that, based on TS content effects to biogas yield, rumen fluid inoculums exhibit the similar effect with other inoculums. Increasing rumen content will also increase biogas production. Due to the optimum total solid (TS) content for biogas production between 7-9 % (or correspond to more and less manure and total liquid 1:1), the rumen fluid content of 50 % will give the best performance for biogas production. The future work will be carried out to study the dynamics of biogas production if both the rumen fluid inoculums and manure are fed in the continuous system


rumen fluid; inoculums; anaerobic digestion; biogas production

Full Text:



. Okkerse, C. and H. V. Bekkum. From fossil to green. Green Chem., 1: 107–114, 1999

. Alvarez, R. and G. Lide´n. 2008. The effect of temperature variation on biomethanation at high altitude, Biores. Technol. 99: 7278–7284, 2008

. Boe, K. and Angelidaki, I. 2009. Serial CSTR digester configuration for improving biogas production from manure, Water Research, 43(1): 166-172, 2009

. Kaparaju, P. and Angelidaki, I., 2008. Effect of temperature and microbial activity on passive separation of digested cattle manure. Bioresour. Technol. 99: 1345–1352, 2008

. Kaparaju, P., Buendía, I., Ellegaard, L., Angelidaki, I., Effects of mixing on methane production during thermophilic anaerobic digestion of manure: labscale and pilot-scale studies. Bioresour. Technol. 99: 4919–4928, 2008

. Liao, P.H., K.V. Lo, S.T. Chieng. 1984. Effect of liquid—solids separation on biogas production from dairy manure. Energy in Agriculture, 3: 61-69, 1984

. Møller, H.B., A.M. Nielsen, R. Nakakubo, H.J. Olsen. Process performance of biogas digesters incorporating pre-separated manure, Livestock Science, 112: 217–223, 2008

. Kayhanian, M. and D. Rich. Pilot-scale high solids thermophilic anaerobic digestion of municipal solid waste with an emphasis on nutrient requirements, Biomass and Bioenergv, 8(6): 433-444, 1995

. Demirci, G. G. and G. N. Demirer. Effect of initial COD concentration, nutrient addition, temperature and microbial acclimation on anaerobic treatability of broiler and cattle manure, Bioresource Technology 93: 109–117, 2004

. Krylova, N.I., R.E. Khabiboulline, R.P. Naumova, and M.A. Nagel, M.A. The influence of ammonium and methods for removal during the anaerobic treatment of poultry manure. J. Chem. Technol. Biotechnol. 70 (1): 99-105

. Callaghan, F.J., D.A.J. Wase, K. Thayanithy, and C.F. Forster. Co-digestion of waste organic solids - batch studies. Bioresource Technol. 67 (2), 117-122, 1999

. Karim, K., R. Hoffmann, K. T. Klassonb, M.H. Al-Dahhan. Anaerobic digestion of animal waste: Effect of mode of mixing, Water Research 39: 3597–3606, 2005

. Lo, K.V., A. J. Whitehead, P. H. Liao, N. R. Bulley. Methane production from screened dairy manure using a fixed-film reactor. Agricultural Wastes, 9(3): 175-188, 1984

. Vartak, D.R., C. R. Engler, M. J. McFarland, and S. C. Ricke. Attached-film media performance in psychrophilic anaerobic treatment of dairy cattle wastewater, Bioresource Technology 62: 79-84, 1997

. Ndegwa, P.M., D.W. Hamilton, J.A. Lalman, and H.J. Cumba. Effects of cycle-frequency and temperature on the performance of anaerobic sequencing batch reactors (ASBRs) treating swine waste, Bioresource Technology, 99: 1972–1980, 2008

. Gelegenis, J., D. Georgakakis, I. Angelidaki, V. Mavris. Optimization of biogas production by co-digesting whey with diluted poultry manure. Renewable Energy, 32(13): 2147-2160, 2007

. Lehtomaki, A., S. Huttunen, J.A. Rintala. Laboratory investigations on co-digestion of energy crops and crop residues with cattle manure for methane production: Effect of crop to manure ratio, Resources, Conservation and Recycling, 51: 591–609, 2007

. Nielsen, H.B. and I. Angelidaki. Strategies for optimizing recovery of the biogas process following ammonia inhibition. Bioresource Technology. 99(17):7995-8001, 2008

. Luengo, P.L. and J. M. Alvarez. Influence of temperature, buffer, composition and straw particle length on the anaerobic digestion of wheat straw-pig manure mixtures. Resources, Conservation and Recycling, 1(1): 27-37, 1988

. Castillo, R.T., P.L. Luengo, and J.M. Alvarez. Temperature effect on anaerobic of bedding manure in a one phase system at different inoculums concentration, Agriculture, Ecosystems and Environment, 54:55-66, 1995

. Sans, C., J. Mata-Alvarez, F. Cecchi, P. Pavan and A. Bassetti. Acidogenic fermentation of organic urban wastes in a plug-flow reactor under thermophilic conditions. Bioresource Technology 54(2): 105-110, 1995

. Lopes, W.S., V.D. Leite and S. Prasad, Influence of inoculum on performance of anaerobic reactors for treating municipal solid waste. Bioresour. Technol., 94: 261-266, 2004

. Forster-Carneiro, T., M. Pérez and L. I. Romero. Influence of total solid and inoculum contents on performance of anaerobic reactors treating food waste. Bioresource Technology 99(15): 6994-7002, 2008

. Aurora, S.P. Microbial Digestion in Ruminants. Indian Council of Agricultural Research, New Delhi, 1983

. Yetilmezsoy, K and S. Sakar. Development of empirical models for performance evaluation of UASB reactors treating poultry manure wastewater under different operational conditions, J. Hazardous Materials, 153: 532–543, 2008

. Nopharatana, A., P. C. Pullammanappallil, W. P. Clarke. Kinetics and dynamic modeling of batch anaerobic digestion of municipal solid waste in a stirred reactor, Waste Management, 27: 595–603, 2007

. Richard, T. The effect of lignin on biodegradability. Cornell Composting, Cornel Waste Management Institute, In, 1996

. Wilkie, A.C. Anaerobic digestion of dairy manure: design and process consideration. in Dairy Manure Management : Tretament, Handling, and Community Relations, Natural Resource, Agriculture, and Engineering Service, Cornell University, Itaca. pp. 301-3, 2005

. Rajeshwari, K.V., M. Balakrishnan, A. Kansal, K. Lata and V.V.N. Kishore. State-of-the-art of anaerobic digestion technology for industrial wastewater treatment. Renew. Sustain. Energy Rev., 4: 135-156, 2000

. Speece, R.E. Anaerobic Technology for Industrial Wastewaters. Archae Press, USA, p.43, ISBN:0-9650226-0-9, 1996

. Balsam, J. Anaerobic digestion of animal wastes: factors to consider. ATTRA-national sustainable agriculture information service. United States Department of Agriculture’s, USA, 2002

. Baserja, U., Biogas production from cowdung: Influence of time and fresh liquid manure. Swiss-Bio Tech., 2: 19-24, 1984

. Zennaki, B.Z., A. Zadi, H. Lamini, M. Aubinear and M. Boulif, Methane Fermentation of cattle manure: Effects of HRT, temperature and substrate concentration. Tropicul. Tural., 14: 134-140, 1996

. Budiyono, I N. Widiasa, S. Johari, and Sunarso. The Kinetic of Biogas Production Rate from Cattle Manure in Batch Mode, International Journal of Chemical and Biological Engineering, 3(1): 39-45, 2010

. Sadaka, S.S. and Engler, C.R. Effect of initial total solids om composting of raw manure with biogas recovery. Compost Sci. and Utilization. 11(4): 361-369, 2003


  • There are currently no refbacks.

Published by Department of Chemical Engineering University of Diponegoro Semarang
Google Scholar

IJSE  by is licensed under Creative Commons Attribution 3.0 License.