Immobilized bacteria by using PVA (Polyvinyl alcohol) crosslinked with Sodium sulfate

Dinh Van Pham, Leu Tho Bach



A new bacteria immobilization technique using PVA (polyvinyl alcohol) crosslink with sodium sulfate was developed. This new technique can simultaneously eliminate the agglomeration of PVA beads and the toxicity of boric acid caused by the PVA-boric methods, also reducing the swelling (when soaking in water) of PVA-boric methods. Beads were immobilized by using four different PVA immobilization processes to create group B, group N, group P and group S. The stability, swelling, relative mechanical strength of these kinds of beads were compared in this study. Only group S was the best and chosen to do experiment for checking survival of bacteria after immobilization process and TOC removal performance of anaerobic reactor. The TOC removal performance of anaerobic reactor achieved 80-87%.



PVA-alginate beads; immobilized bacteria; bacterial gel bead

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. Vojtisek, V. and V. Jirku, (1983). Immobilized cells. Folia Microbiol (Praha). 28(4): p. 309-40.

. Ariga, O., et al., (1987). Immobilization of Microorganisms with Pva Hardened by Iterative Freezing and Thawing. Journal of Fermentation Technology. 65(6): p. 651-658.

. Kuu, W.Y. and J.A. Polack, (1983). Improving Immobilized Biocatalysts by Gel Phase Polymerization. Biotechnology and Bioengineering. 25(8): p. 1995-2006.

. Freeman, A. and Y. Aharonowitz, (1981). Immobilization of Microbial-Cells in Crosslinked, Pre-Polymerized, Linear Polyacrylamide Gels - Antibiotic Production by Immobilized Streptomyces-Clavuligerus Cells. Biotechnology and Bioengineering. 23(12): p. 2747-2759.

. Hashimoto, S. and K. Furukawa, (1987). Immobilization of Activated-Sludge by Pva Boric-Acid Method. Biotechnology and Bioengineering. 30(1): p. 52-59.

. Ramaraj, B., (2007). Crosslinked poly(vinyl alcohol) and starch composite films. II. Physicomechanical, thermal properties and swelling studies. Journal of Applied Polymer Science. 103(2): p. 909-916.

. Khoo, K.M. and Y.P. Ting, (2001). Biosorption of gold by immobilized fungal biomass. Biochemical Engineering Journal. 8(1): p. 51-59.

. Hassan, C.M. and N.A. Peppas, (2000). Cellular PVA hydrogels produced by freeze/thawing. Journal of Applied Polymer Science. 76(14): p. 2075-2079.

. Gholap, S.G., J.P. Jog, and M.V. Badiger, (2004). Synthesis and characterization of hydrophobically modified poly(vinyl alcohol) hydrogel membrane. Polymer. 45(17): p. 5863-5873.

. Wu, K.Y.A. and K.D. Wisecarver, (1992). Cell Immobilization Using Pva Cross-Linked with Boric-Acid. Biotechnology and Bioengineering. 39(4): p. 447-449.

. Zain, N.A.M., M.S. Suhaimi, and A. Idris, (2011). Development and modification of PVA-alginate as a suitable immobilization matrix. Process Biochemistry. 46(11): p. 2122-2129.

. Choorit, W. and P. Wisarnwan, (2007). Effect of temperature on the anaerobic digestion of palm oil mill effluent. Electronic Journal of Biotechnology. 10(3): p. 376-385.

. Bach, L.T., Z.I. Bhatti, and K. Furukawa, (2002). Anaerobic Treatment of Sugary Wastewater by the UASB Process. Japanese Journal of Water Treatment Biology. 38(1): p. 11-20.

. Tchobanoglous, G., et al. (2003). Wastewater Engineering: Treatment and Reuse. Vol. 4: McGraw-Hill.

. Chang, C.-C. and S.-K. Tseng, (1998). Immobilization of Alcaligenes eutrophus using PVA crosslinked with sodium nitrate. Biotechnology Techniques. 12(12): p. 865-868.

. Chen, K.-C. and J.-Y. Houng, (1997). Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel. Immobilization of Enzymes and Cells. 1: p. 207-216.

. Pillay, V., W. Sibanda, and M.P. Danckwerts, (2005). Sequential design of a novel PVA-based crosslinked ethylenic homopolymer for extended drug delivery. International Journal of Pharmaceutics. 301(1-2): p. 89-101.

. Gacesa, P. and N.J. Russell. (1990). The structure and properties of alginate. Pseudomonas Infection and Alginates, ed. P. Gacesa and N.J. Russell. Springer Netherlands. 29-49.

. Shibayama, M., et al., (1988). Sol-gel transition of poly(vinyl alcohol)-borate complex. Polymer. 29(11): p. 2066-2071.

ISO 10532:2008 – Water quality – Determination of pH.

ISO 8245: 1999 - Water quality – Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC).

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