skip to main content

STUDY ON CHANGES OF SPERM HEAD MORPHOMETRY AND DNA INTEGRITY OF FREEZE-DRIED BOVINE SPERMATOZOA

*S. Said  -  Research Centre for Biotechnology, Indonesian Institute of Sciences, Indonesia
F. Afiati  -  Research Centre for Biotechnology, Indonesian Institute of Sciences, Indonesia
T. Maulana  -  Research Centre for Biotechnology, Indonesian Institute of Sciences, Indonesia

Citation Format:
Abstract

Changes of sperm heads morphometric and DNA integrity of freeze-dried bovine spermatozoa were investigated. Freeze-dried spermatozoa had stored in the refrigerator at 4°C for 2 years. Computer-assisted sperm analysis (CASA) was used in this study to identify sperm head morphometry, while for DNA integrity analysis using acridine orange staining. Samples were smeared on glass slides, fixed for 2 h in acetic alcohol and stained with acridine orange solution. After staining, each slide was examined at x400 magnification in a fluorescence microscope with axio vision (Zeiss Company, Germany). Proportion of fluorescence red and green emissions of the sperm head were examined and scored. These results indicated that sperm head had enlarged significantly (P<0.05) after freeze-drying process. However, freeze-dried sperm heads morphometry significantly (P<0.05) decrease after incubation for 3 and 6 hours. Changes of DNA integrity of freeze-dried spermatozoa significantly (P<0.05) decrease after incubation for 6 hours. In the present study concluded that (1) freeze-drying spermatozoa caused sperm head morphometric enlarged, whereas incubation time caused sperm heads decreased, (2) DNA integrity of freeze-dried sperm head is still intact during incubation 3 hours, and decreased DNA integrity occur in incubation for 6 hours.

Fulltext View|Download
Keywords: morphometry; DNA integrity; freeze-dried spermatozoa; bovine; incubation

Article Metrics:

Last update:

  1. Sperm quality profile of Bali polled bull

    H. Hasbi, M. I. A. Dagong, Zulkharnaim, S. Baba, H. Sonjaya, S. Baco, S. Gustina, T. Maulana, M. Gunawan, P. P. Agung, N. Herlina, N. D. Yanthi, E. M. Kaiin, S. Said. PROCEEDINGS OF THE 4TH INTERNATIONAL CONFERENCE OF ANIMAL SCIENCE AND TECHNOLOGY (ICAST 2021), 2628 , 2023. doi: 10.1063/5.0144042
  2. Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen

    Ani Atul Arif, Tulus Maulana, Ekayanti Mulyawati Kaiin, Bambang Purwantara, Raden Iis Arifiantini, Erdogan Memili. Veterinary World, 13 (11), 2020. doi: 10.14202/vetworld.2020.2422-2428
  3. Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility

    Hasbi Hasbi, Hikmayani Iskandar, Herry Sonjaya, Bambang Purwantara, Raden Iis Arifiantini, Muhammad Agil, Berlin Pandapotan Pardede, Suyadi Suyadi, Wike Andre Septian, Daud Samsudewa, Erni Damayanti, Tulus Maulana, Syahruddin Said. Veterinary World, 2024. doi: 10.14202/vetworld.2024.593-601

Last update: 2024-07-15 15:49:30

  1. Effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa

    Said S.. Journal of the Indonesian Tropical Animal Agriculture, 45 (3), 2020. doi: 10.14710/jitaa.45.3.189-196
  2. Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen

    Ani Atul Arif, Tulus Maulana, Ekayanti Mulyawati Kaiin, Bambang Purwantara, Raden Iis Arifiantini, Erdogan Memili. Veterinary World, 13 (11), 2020. doi: 10.14202/vetworld.2020.2422-2428
  3. Numerical Evaluation of Residual Water Content after Freezing during the Lyophilization of Platelets

    Zhang S.. Fluid Dynamics and Materials Processing, 16 (6), 2020. doi: 10.32604/fdmp.2020.010352