Preliminary study of solute carrier family 23 member 3 (SLC23A3) gene as candidate marker for fatty acid traits in Kampung-Broiler crossbred chickens

DOI: https://doi.org/10.14710/jitaa.43.3.201-210

Article Metrics: (Click on the Metric tab below to see the detail)

Article Info
Submitted: 13-03-2018
Published: 23-08-2018
Section: Articles
Fulltext PDF Tell your colleagues Email the author

SLC23A3 is one of the key genes which control the properties of the of fatty acids content in the meat. The aim of this study was to identify the association of SNP and mRNA expression of SLC23A3 as a candidate marker for fatty acid traits (FAs). Sixty two F2 Kampung × Broilers crossbred chickens population were used in this study. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. The PCR-RFLP and qRT-PCR were used for association study and expression analysis of SLC23A3 gene with divergent fatty acid composition. A SNP in coding region c.22385690 A>C of the SLC23A3 gene was associated (P<0.05) with fatty acid composition including stearic acid (C18:0), elaidic acid (C18:1n9t), and linoleic acid (C18:2n6c). The SLC23A3 was detected in liver from high fatty acids (HFA) and low fatty acid (LFA) composition. However, gene expression of SLC23A3 were not differentially expressed between HFA and LFA. These results will explain better understanding of the key important role of the SLC23A3 in fatty acid traits within the liver and will propose SLC23A3 as a potential genomic selection for selection of chickens with fatty acid composition.


Keywords

polymorphism; mRNA expression; SLC22A3; selection; unsaturated fatty acid

  1. A. Gunawan 
    Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680, Indonesia
  2. S. Y. Basril 
    Graduate School of Bogor Agricultural University, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680 Bogor, Indonesia
  3. K. Listyarini 
    Graduate School of Bogor Agricultural University, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680 Bogor, Indonesia
  4. A. Furqon 
    Graduate School of Bogor Agricultural University, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680 Bogor, Indonesia
  5. W. Bilyaro 
    Graduate School of Bogor Agricultural University, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680 Bogor, Indonesia
  6. J. Jakaria 
    Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680, Indonesia
  7. M. J. Uddin 
    School of Veterinary Science, The University of Queensland Gatton Campus, Gatton  Qld 4343, Australia
  8. C. Sumantri 
    Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680, Indonesia