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Antibody response to Newcastle disease virus recombinant fusion protein in post-vaccinated laying hens

J. Jawad  -  Master Study Program of Biology, Post Graduate Program, Faculty of Biology, Universitas Gadjah Mada, Indonesia
R. W. Astuti  -  Research Center for Biotechnology, Universitas Gadjah Mada, Indonesia
A. Haryanto  -  Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia
*N. Wijayanti orcid scopus publons  -  Animal Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia
Open Access Copyright (c) 2023 Journal of the Indonesian Tropical Animal Agriculture
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

This research was aimed to analyze antibody response in laying hens post vaccinated by Newcastle Disease Virus (NDV) recombinat Fusion (F) protein which has been succesfully expressed from the F gene of local isolates of NDV from Kulon Progo strain (0663/04/2013), Yogyakarta, Indonesia. The F gene cloned into expression vector plasmid pBT7-N-His. Two types of NDV recombinant vaccine, a concentrated and pure F recombinant protein were used for vaccination. A concentrated recombinant F protein was collected from the centrifugal ultrafiltration process and a pure recombinant F protein was collected from the electroeluted process. Recombinant F protein of NDV was successfully expressed, purified, and visualized by Sodium Dodecyl Sulphate–Polyacrylamide Gel Electrophoresis (SDS-PAGE) with Coomassie Brilliant Blue staining with a molecular weight of 28 kDa. All two types of recombinant F vaccines and a commercial live vaccine as a positive control were injected two times at 14 and 18th weeks old laying hens to analyze the antibody response in serum. In comparison with a commercial live NDV vaccine, indirect Enzyme-linked Immunosorbent Assay (ELISA) revealed that antibody responses were high in both recombinant F protein vaccinated groups. In conclusion, the recombinant F protein has the potential to be developed as a recombinant vaccine candidate to obtain a higher antibody response in laying hens compared to commercially available live NDV vaccines.

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Subject ELISA; Laying hens; NDV; Post-vaccinated; recombinant F protein;
Type Other
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Keywords: ELISA; Laying hens; NDV; Post-vaccinated; Recombinant F protein
Funding: The Gadjah Mada International Fellowship (GMIF) program

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