skip to main content

GENETIC DIVERSITY OF KEJOBONG GOAT BASED ON MITOCHONDRIAL DNA D-LOOP SEQUENCE

*M. F. Harlistyo  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
S. Sutopo  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
E. Kurnianto  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia

Citation Format:
Abstract
This study was aimed to find out the diversity of mtDNA D-loop at Kejobong goat. The completemtDNA D-loop sequence of 12 goat blood samples were analyzed from 4 different location inPurbalingga Regency, Central Java province, sub-districts Kejobong, Pangadegan, Bukateja, andKaligondang. The mtDNA D-loop was extracted from blood sample. DNA obtained were amplified byPCR (Polymerase Chain Reaction) method using primers (5’-tcactatcagcacccaaagc-3’) as forward and(5’-ggcattttcagtgccttgct-3’) as reverse and subsequently sequenced. After nucleotide sequencing analysisconducted, 548 bp along was obtained. Nucleotides were then aligned with Capra hircus (GenBankAccess No.: KF952601.1) and apparently there were 11 different sites on the segment of mtDNA Dloop.Five sites could be used as a specific marker to distinguish between the Capra hircus andKejobong goat, namely at the site of 317 (A-G), 403 (T-C), 434 (T-C), 537 (C-T), and 553 (A-G).Nucleotide sequence analysis also contained seven different haplotypes. It was concluded that thedistribution of the different sites showed different haplotype patterns in Kejobong goat.
Fulltext View|Download
Keywords: genetic diversity; Kejobong goat; mtDNA D-loop

Article Metrics:

Last update:

  1. Study of Kosta goat (Capra hircus) mitochondrial DNA and their phylogenetic based on whole genome sequensing

    Nena Hilmia, Dedi Rahmat, Muhammad Ihsan Andi Dagong, Sri Rachma Aprilita Bugiwati, Sutopo Sutopo, Dela Ayu Lestari, Asep Setiaji, Procula Rudlof Matitaputty, Sutikno Sutikno, Hideyuki Mannen. Small Ruminant Research, 221 , 2023. doi: 10.1016/j.smallrumres.2023.106937
  2. Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

    Dela Ayu Lestari, Endang Purbowati, Sutopo Sutopo, Edy Kurnianto. Veterinary World, 11 (8), 2018. doi: 10.14202/vetworld.2018.1196-1202

Last update: 2024-11-05 07:36:36

  1. Phylogenetical relationships between Kejobong goat and other goats based on Mt-DNA D-loop sequence analysis

    Lestari D.. Tropical Animal Science Journal, 41 (2), 2018. doi: 10.5398/tasj.2018.41.2.85
  2. Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

    Dela Ayu Lestari, Endang Purbowati, Sutopo Sutopo, Edy Kurnianto. Veterinary World, 11 (8), 2018. doi: 10.14202/vetworld.2018.1196-1202