DOI: https://doi.org/10.14710/jitaa.38.4.207-211

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

*W.P.B. Putra  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
T. Hartatik  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
S. Sumadi  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia

Citation Format:
Abstract
The objective of this research was to identify growth hormone (GH) genes genotype in selected
Aceh cattle at Indrapuri’s Breeding and Forage Centre (IBFC) of Aceh Cattle. Fourty one cattle
consisting of 21 male and 20 female cattle were used in this study. The genomic DNA was extracted
from blood using Sambrook et al. (1989) methods. Polymerase Chain Reaction - Restriction Fragment
Length Polymorphism (PCR-RFLP) and mehod of sequencing was used to detect MspI site on GH gene.
Based on sequencing results, it can be concluded that all cattle were monomorphism. The frequency of
TT genotype were 1.00 and same as T allele frequency. The transition of C (cytosine) into T (thymine)
on 1548 bp caused the lost of restriction site.
Fulltext View|Download
Keywords: Aceh cattle; GH gene; PCR-RFLP; sequencing; MspI restriction enzyme

Article Metrics:

Article Info
Section: Articles
Language : EN
Recent articles
MULTIVARIATE ANALYSIS ON CRANIUM MEASUREMENTS OF THREE BREEDS OF GOAT IN CENTRAL JAVA MILK CONSUMPTION BEHAVIOR IN HOUSEHOLD HAVING CHILDREN IN GROWING AGE IN YOGYAKARTA SPECIAL PROVINCE PURIFICATION OF ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDE DERIVED FROM KACANG GOAT MEAT PROTEIN HYDROLYSATE More recent articles
Most cited articles
SUPPLEMENTATION OF VITAMIN E AND C IN FEED ON MEAT QUALITY, THIOBARBITURIC ACID REACTIVE SUBSTANCE (TBARS) AND MYOGLOBIN LEVEL OF MUSCOVY DUCK MEAT The effect of hatchery waste meal in ration on nutrient retention and performances of laying quails The effect of coating of edible film from bovine split hide gelatin on beef meatballs properties ASSESSMENT OF METHANE ESTIMATION FROM VOLATILE FATTY ACID STOICHIOMETRY IN THE RUMEN IN VITRO SINGLE NUCLEOTIDE POLYMORPHISMS OF LIPOPROTEIN LIPASE GENE AND ITS ASSOCIATION WITH MARBLING QUALITY IN LOCAL SHEEPS More cited articles

Last update:

  1. Growth hormone-Msp1 loci polymorphism and its association with first lactation traits in Sahiwal cattle

    SHWETA SACHAN, I D GUPTA, ARCHANA VERMA, M R VINEETH, REBEKA SINHA. The Indian Journal of Animal Sciences, 90 (4), 2020. doi: 10.56093/ijans.v90i4.104226

Last update: 2024-11-21 03:37:57

  1. Growth hormone-Msp1 loci polymorphism and its association with first lactation traits in Sahiwal cattle

    SHWETA SACHAN, I D GUPTA, ARCHANA VERMA, M R VINEETH, REBEKA SINHA. The Indian Journal of Animal Sciences, 90 (4), 2020. doi: 10.56093/ijans.v90i4.104226

  2. Review: Genetic diversity of local and exotic cattle and their crossbreeding impact on the quality of Indonesian cattle

    Sutarno . Biodiversitas, 16 (2), 2015. doi: 10.13057/biodiv/d160230

  3. Effect of genetic polymorphisms in GH/HpaII and MSTN/DraI loci on body weight in friesian bull calves

    Nasr S.. Pakistan Journal of Biological Sciences, 19 (8), 2016. doi: 10.3923/pjbs.2016.338.344

  4. Genotype analysis of partial growth hormone gene (GH891MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

    Hartatik T.. Journal of the Indonesian Tropical Animal Agriculture, 43 (1), 2018. doi: 10.14710/jitaa.43.1.1-8