GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

*W.P.B. Putra -  Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No.3 Bulaksumur, Sleman, Yogyakarta 55281, Indonesia
T. Hartatik -  Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No.3 Bulaksumur, Sleman, Yogyakarta 55281, Indonesia
S. Sumadi -  Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No.3 Bulaksumur, Sleman, Yogyakarta 55281, Indonesia
Published: 1 Dec 2013.
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Abstract
The objective of this research was to identify growth hormone (GH) genes genotype in selected
Aceh cattle at Indrapuri’s Breeding and Forage Centre (IBFC) of Aceh Cattle. Fourty one cattle
consisting of 21 male and 20 female cattle were used in this study. The genomic DNA was extracted
from blood using Sambrook et al. (1989) methods. Polymerase Chain Reaction - Restriction Fragment
Length Polymorphism (PCR-RFLP) and mehod of sequencing was used to detect MspI site on GH gene.
Based on sequencing results, it can be concluded that all cattle were monomorphism. The frequency of
TT genotype were 1.00 and same as T allele frequency. The transition of C (cytosine) into T (thymine)
on 1548 bp caused the lost of restriction site.
Keywords
Aceh cattle; GH gene; PCR-RFLP; sequencing; MspI restriction enzyme

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