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Molecular Characterization Of Phylloplane Mold From Avicennia marina Leaves

*Arina Tri Lunggani  -  Department of Biology, Faculty of Science and Mathematics, Diponegoro University,, Indonesia
Wahyu Aji Mahardhika  -  Study Program of Microbiology, Graduate School of Bogor Agricultural University, Indonesia
Adi Budi Utomo  -  Department of Biology, Faculty of Science and Mathematics, Diponegoro University,, Indonesia
Endang Kusdiyantini  -  Department of Biology, Faculty of Science and Mathematics, Diponegoro University,, Indonesia

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Abstract

Mangroves are a habitat for organisms and microorganisms, including phylloplane molds. Phylloplane molds are known to have various potentials such as antimicrobial, enzyme, and pigment-producing. PFM19 is an orange pigment-producing phylloplane mold. Identification of the mold is needed to determine the species of the fungus so that it can be used for further research. This study aims to identify molecularly the PFM19 mold that produces orange pigment using ITS markers. The methods used in this study included the rejuvenation of isolates, DNA extraction, DNA amplification, and phylogenetic analysis. The results obtained that PFM19 has similarities with Talaromyces islandicus CBS 388.48 by 100% based on ITS markers.

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Keywords: phylloplane mold, molecular characterization, ITS

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Last update: 2024-03-28 17:20:23

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