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Commercial Probiotics Improve Growth, Feed Efficiency, Nitrogen Removal, Hemocyte Count and Suppression of Vibrio Population in Pacific White Shrimp Culture

1Department of Aquaculture, Marine and Fisheries Polytechnic of Jembrana, Indonesia

2Study Program of Fish Breeding Technology, State Polytechnic of Lampung, Indonesia

3Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Indonesia

4 Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University , Indonesia

5 Laboratory of Aquaculture and Artemia Reference Center, Faculty of Bioscience Engineering, Belgium

6 Regenerative Aquaculture Research Group, Research Organization for Agriculture and Food, Indonesian National Research and Innovation Agency, Indonesia

7 Geography Department, University of Hawai’i at Manoa, United States

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Received: 4 Jun 2025; Revised: 1 Jul 2025; Accepted: 16 Jul 2025; Available online: 3 Sep 2025; Published: 10 Sep 2025.

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Abstract

The Pacific white shrimp (Penaeus vannamei) is a key species in global aquaculture, particularly under intensive farming systems where high stocking densities often lead to deteriorating water quality, disease outbreaks, and reliance on antibiotics. While probiotics are increasingly applied to enhance shrimp health and performance, comparative evidence on the efficacy of different commercial probiotic formulations remains limited. This study aimed to evaluate the effects of three commercial probiotic products on growth performance, water quality, microbial populations, and immune response in intensively reared Pacific white shrimp. Shrimp were fed diets supplemented with one of three probiotics (PB, PL, and PMB) or a control diet for 40 days. Growth parameters, nitrogenous waste levels, bacterial counts in water and organs, and total hemocyte count (THC) were analyzed. The PB treatment (multi-strain Bacillus) significantly enhanced specific growth rate (2.99±0.0027% day¹), feed efficiency (57.5±0.09%), weight gain, and THC (3.80 × 10 cells mm³) compared to control (P<0.05). The PB and PMB treatments also reduced total ammonia nitrogen, nitrite, nitrate, and Vibrio populations in water and shrimp tissues. Intestinal colonization by beneficial bacteria was highest in PB and PL groups. These results demonstrate that specific probiotic formulations, particularly PB, can improve growth, immunity, and water quality while reducing reliance on antibiotics and water exchange. The findings support the integration of targeted probiotics in sustainable shrimp aquaculture, including biofloc and recirculating aquaculture systems (RAS).

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Keywords: Bacillus; Lactobacillus; Mixed Bacteria; Penaeus vannamei; Probiotics

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