DOI: https://doi.org/10.14710/jitaa.42.3.135-146

Amino acid diversity on the basis of cytochrome b gene in Kacang and Ettawa Grade goats

*D. A. Lestari  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
S. Sutopo  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
E. Kurnianto  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
Open Access Copyright (c) 2017 Journal of the Indonesian Tropical Animal Agriculture under https://creativecommons.org/licenses/by-sa/4.0/.

Citation Format:
Abstract

The objectives of study were to identify and assess the amino acid diversity of Cytochrome b (Cyt b) gene, genetic marker and characteristic of specific amino acid in Kacang and Ettawa Grade goat. Nineteen heads of Kacang goat (KG) and twelve heads of Ettawa Grade goat (EG) were purposively sampled. The genomic DNA was isolated by Genomic DNA Mini Kit (Geneaid) and amplified Cyt b using PCR method with CytbCapF and CytbCapR primers and was sequenced. The results showed that there were two specific amino acids that distinguish KG and EG goat with C. hircus and C. aegagrus and four specific amino acids that distinguish KG and EG goat with C. falconeri, but there were no specific amino acids can be used as a genetic marker to distinguish between Kacang and EG goat. In conclusion, specific amino acids in Cyt b gene can be used as a genetic marker among KG and EG goat with 3 goat others comparator.

Fulltext View|Download
Keywords: amino acid; cytochrome b; genetic marker; local goat

Article Metrics:

Article Info
Section: Articles
Language : EN
Recent articles
Evaluation of feeding Indigofera zollingeriana leaf meal and Sardinella lemuru fish oil on lipids metabolism of local ducks Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia Comparative performance of Landrace, Yorkshire and Duroc breeds of swine Back-matter (September 2017) Front-matter (September 2017) More recent articles
Most cited articles
Effect of binder mineral in batchery waste based feed pellet on its proximate component and energy values FORAGE PRODUCTIVITY OF ARBILA (Phaseolus lunatus) AT VARIOUS LEVELS OF RHIZOBIUM INOCULANTS AND HARVESTING TIMES Urea treatment of rice straw at elevated temperature and pressure: Effects on fiber content, rumen fermentation and digestibility MYOSTATIN GENE ANALYSIS IN THE FIRST GENERATION OF THE BELGIAN BLUE CATTLE IN INDONESIA Cholesterol Assimilation of Saccharomyces cerevisiae B-18 isolated from gastrointestinal tract of Javanese duck More cited articles

Last update:

  1. Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences

    Alek Ibrahim, Endang Baliarti, I Gede Suparta Budisatria, Wayan Tunas Artama, Rini Widayanti, Dyah Maharani, Luis Tavares, Endang Tri Margawati. Journal of Genetic Engineering and Biotechnology, 21 (1), 2023. doi: 10.1186/s43141-023-00491-z

  2. Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

    Dela Ayu Lestari, Endang Purbowati, Sutopo Sutopo, Edy Kurnianto. Veterinary World, 11 (8), 2018. doi: 10.14202/vetworld.2018.1196-1202

Last update: 2023-09-30 11:03:53

  1. Genetic variation of cytochrome b gene in three local goat breeds in Central Java of Indonesia

    Sutopo S.. Iranian Journal of Applied Animal Science, 8 (4), 2018.

  2. Phylogenetical relationships between Kejobong goat and other goats based on Mt-DNA D-loop sequence analysis

    Lestari D.. Tropical Animal Science Journal, 41 (2), 2018. doi: 10.5398/tasj.2018.41.2.85

  3. Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

    Dela Ayu Lestari, Endang Purbowati, Sutopo Sutopo, Edy Kurnianto. Veterinary World, 11 (8), 2018. doi: 10.14202/vetworld.2018.1196-1202