skip to main content

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

*T. Hartatik  -  Laboratory of Animal Genetics and Breeding, Faculty of Animal Science, Universitas Gadjah Mada, Indonesia
D. E. Putra  -  Laboratory of Animal Genetics and Breeding, Faculty of Animal Science, Andalas University, Indonesia
S. D. Volkandari  -  Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Indonesia
T. Kanazawa  -  Department of Animal Cell Science and Engineering, School of Agriculture, Ibaraki University, Japan
S. Sumadi  -  Laboratory of Animal Genetics and Breeding, Faculty of Animal Science, Universitas Gadjah Mada, Indonesia
Open Access Copyright (c) 2018 Journal of the Indonesian Tropical Animal Agriculture under https://creativecommons.org/licenses/by-sa/4.0/.

Citation Format:
Abstract

Growth hormone gene that controls metabolism and body size of animals and polymorphism of the gene is known related to performance of livestock. Identification of gene polymorphisms was important to get early information to determine genetic markers associated with economically desirable traits. Genetic mapping is one of important characterizations in the selection process. Pesisir cattle is one of local cattle in Indonesia that are reared in West Sumatera and have become adaptive to local environment. The present study aimed to identify the genotype of Growth Hormone gene (GH891│MspI) of the Pesisir cattle and crossbred Simmental-Pesisir cattle (designated as SimPes) and relationship between growth performance (body weight and body size). The present study was conducted to 30 blood samples consisted of 15 Pesisir cattle and 15 SimPes cattle. Body weight and body size were measured at 12-18 months of age. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used in this research. The results showed that there exist three variant genotypes (MspI-/-, MspI+/-, MspI+/+) and that allele frequencies of MspI- and MspI+ were 53.3% and 46.7%, respectively. Non-significant effect has found between GH’s genotype and growth traits (Body measurement and body weight) in both of breed cattle. In conclusion, three variant genotypes (GH891│MspI) in Pesisir and SimPes (Simmental x Pesisir) cattle population were found and no significant effect on growth trait performance.

Fulltext View|Download
Keywords: genotype; MspI enzyme; growth hormone gene; growth trait; Pesisir cattle; SimPes cattle

Article Metrics:

Last update:

  1. Meta-analysis of SNP in growth hormone gene associated with milk traits in dairy cows

    Yogesh C. Bangar, Ankit Magotra. Tropical Animal Health and Production, 53 (2), 2021. doi: 10.1007/s11250-021-02670-x
  2. Sumbawa cattle: a study of growth hormone (GH) gene variants and their association with biometric traits

    P. W. Prihandini, H. Hasinah, A. P. Z. N. L. Sari, Y. A. Tribudi, L. Praharani, S. A. Asmarasari, E. Handiwirawan, B. Tiesnamurti, D. K. Robba, E. Romjali, A. Ibrahim. Brazilian Journal of Biology, 84 , 2024. doi: 10.1590/1519-6984.282823
  3. Detection of growth hormone (GH|MspI,GHR|AluI, Pit1|HinfI) genes polymorphism and its association with body weight of Grati-Bali Cattle (Bos sondaicus)

    Hartati, M Luthfi, D Ratnawati, B D P Soewandi. IOP Conference Series: Earth and Environmental Science, 788 (1), 2021. doi: 10.1088/1755-1315/788/1/012010
  4. Identification of growth hormone gene polymorphism and its association with body weight in PO Kebumen cattle

    B D P Soewandi, Hartati, A A R Hapsari. IOP Conference Series: Earth and Environmental Science, 637 (1), 2021. doi: 10.1088/1755-1315/637/1/012072
  5. Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

    Sihem Amiri, Bayrem Jemmali, Mohamed Amine Ferchichi, Hajer Jeljeli, Rekik Boulbaba, Abderrahmane Ben Gara. Archives Animal Breeding, 61 (4), 2018. doi: 10.5194/aab-61-481-2018

Last update: 2024-11-21 20:25:19

No citation recorded.