The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition

I. Darussalam; R. I. Arifiantini; I. Supriatna; R. S. D. Rasad

doi:10.14710/jitaa.45.3.197-205

DOI: https://doi.org/10.14710/jitaa.45.3.197-205

The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition

I. Darussalam - Reproductive Biology Program, Graduate School of IPB University, Indonesia

*R. I. Arifiantini

- Division of Reproduction and Obstetrics, Department of Veterinary Clinic, Reproduction and Pathlogy, Faculty of Veterinary Medicine, IPB University, Indonesia

I. Supriatna - Division of Reproduction and Obstetrics, Department of Veterinary Clinic, Reproduction and Pathlogy, Faculty of Veterinary Medicine, IPB University, Indonesia

R. S. D. Rasad - Laboratory of Animal Reproduction and Artificial Insemination, Faculty of Animal Husbandry, Padjadjaran University, Indonesia

BibTex Citation Data :

@article{JITAA29250,
    author = {I. Darussalam and R. I. Arifiantini and I. Supriatna and R. S. D. Rasad},
    title = {The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition},
    journal = {Journal of the Indonesian Tropical Animal Agriculture},
  volume = {45},
    number = {3},
    year = {2020},
    keywords = {L-Carnitine; liquid semen; Pasundan bulls; Tris-egg yolk diluents},
    abstract = {     The       study       was       conducted to investigate      the effect of L-Carnitine supplementation in Tris      -      egg      yolk (TEY) diluents to optimize the quality of       P      asundan bull       liquid       semen.       S      emen       samples were       collected from three       P      asundan bull      s (6 – 7 years old)      using      an      artificial vagina. Semen       samples       were evaluated macroscopically and microscopically      , semen having ≥70% progressive motility, ≥500×10       6       ml       -1        sperm concentration and ≤ 20% sperm abnormalities were       divided into 5 equal part. Each       part        was      diluted with TEY      and supplemented with      0 mM      (control), 1 mM, 2 mM, 3 mM and 4 mM L-Carnitine.       Liquid      semen was stored      at      5      °C.      Sperm       motility was evaluated every 12 h        objectively using Computer Asissted Sperm Analysis (CASA      )       until       progressive motility      reached       40%. The best diluent       was       demonstrated      by progressive motility (PM)      by TEY supplemented with 1 mM L-Carnitine      (43.08±0.49      %      )       that       remained up to 108 h compared to      the      control group (43.63±0.70      %      ) that remained up to 72 h (P<0      .      05). In conclusion, 1 mM L-Carnitine       in       TEY was the best       concentration       for        preservation of       P      asundan bull semen       stored in the liquid form.       },
   issn = {2460-6278},   pages = {197--205}  doi = {10.14710/jitaa.45.3.197-205},
    url = {https://ejournal.undip.ac.id/index.php/jitaa/article/view/29250}
}

Citation Format:

Abstract

The study was conducted to investigatethe effect of L-Carnitine supplementation in Tris-eggyolk (TEY) diluents to optimize the quality of Pasundan bull liquid semen. Semen samples were collected from three Pasundan bulls (6 – 7 years old)usinganartificial vagina. Semen samples were evaluated macroscopically and microscopically, semen having ≥70% progressive motility, ≥500×10⁶ml^-1 sperm concentration and ≤ 20% sperm abnormalities were divided into 5 equal part. Each part wasdiluted with TEYand supplemented with0 mM(control), 1 mM, 2 mM, 3 mM and 4 mM L-Carnitine. Liquidsemen was storedat5°C.Sperm motility was evaluated every 12 h objectively using Computer Asissted Sperm Analysis (CASA) until progressive motilityreached 40%. The best diluent was demonstratedby progressive motility (PM)by TEY supplemented with 1 mM L-Carnitine(43.08±0.49%) that remained up to 108 h compared tothecontrol group (43.63±0.70%) that remained up to 72 h (P<0.05). In conclusion, 1 mM L-Carnitine in TEY was the best concentration for preservation of Pasundan bull semen stored in the liquid form.

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Keywords: L-Carnitine; liquid semen; Pasundan bulls; Tris-egg yolk diluents

Funding: This paper is supported by USAID Through Sustainable Higher Education Research Alliances (SHERA) Program – Center for Collaborative Research Animal Biotechnology and Coral Reef Fisheries (CCR ANBIOC

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In Vol 45, No 3 (2020): September

The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition

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