Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation

M. T. Herbowo  -  Biology of Reproduction, Post Graduate School of IPB University, Indonesia
*R. I. Arifiantini scopus  -  Division of Reproduction and Obstetrics, Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University, Indonesia
N. W. K. Karja  -  Division of Reproduction and Obstetrics, Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University, Indonesia
R. G. Sianturi  -  Indonesian Research Institute for Animal Production, Indonesia
Received: 26 Mar 2020; Accepted: 17 Jun 2020; Published: 18 Dec 2020.
DOI: https://doi.org/10.14710/jitaa.45.4.268-276 View
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Abstract

Present study aimed to determine the effectivenessof dimethylformamide (DMF)as cryoprotective agent (CPA) by determining its optimum concentration inTris Eggyolk (TEY) extender on the quality of post-thaw swamp buffalo semen. Only ejaculates having > 60% sperm motility were divided into eight s tubes. Four tubes were diluted in TEY with 4, 5, 6, 7% of glycerol and four other tubes were diluted in TEY with 4, 5, 6, 7% of DMF. Diluted semen then packed into ministraw (0.25 mL), equilibrated at 4°C for 4 hours and freezed at 8 cm above liquid nitrogen vapour for 10 minutes and then stored at liquid nitrogencontainer. After 24 hours of storage, straws were thawed at 37°C for 30 seconds. The result showed that sperm motility after equilibration tend to be the same at all four glycerol concentrations, whereas the decrease in motility was highest in extenders with a DMF concentration of 7% (P <0.05). Post-thaw sperm motility and recovery rate in TEY extender containing 5% DMF or 7% glycerol were highest (P<0.05) than other glycerol or DMF concentration. Sperm viability and membrane integrity did not differ among TEY containing 6% or 7% glycerol, both  were higher (P<0.05) than 4% or 5% glycerol. Viability and membrane integrity of sperm in 5% DMF was superior (P<0.05) to 4%, 6% or 7%. This research concluded that 5% DMF or 7% glycerol was considered to be the most suitable CPAs for swamp buffalo semen cryopreservation.

 

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Keywords: cryoprotectant; dimethylformamide; glycerol; sperm; swamp buffalo
Funding: The authors acknowledge the Indonesian Endowment Fund for Education, Indonesian Ministry of Finance for providing the funding

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