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Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia

*E. T. Setiatin  -  Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Indonesia
D. A. Lestari  -  Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Indonesia
D. W. Harjanti  -  Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Indonesia
A. Setiaji  -  Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Indonesia
E. Kurnianto  -  Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Indonesia
Open Access Copyright (c) 2023 Journal of the Indonesian Tropical Animal Agriculture
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.

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Keywords: FSHR; Genetic marker; Local goat; Prolific; SNPs

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