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@article{JITAA52455, author = {A. Ismail and A. I. Muhammad and L. T. Kee and L. T. Chwen and A. A. Samsudin}, title = {Effects of rumen-protected fat on changes of metabolites and reproductive genes in testes of Malin rams}, journal = {Journal of the Indonesian Tropical Animal Agriculture}, volume = {48}, number = {2}, year = {2023}, keywords = {Energy; Fat; Gene expression; Metabolites; Sheep}, abstract = {The effects of RPF on metabolites and reproductive genes in testes of Malin ram was investigated. Twenty Malin rams (36.6kg ± 5.57 kg of bodyweight), were subjected to four dietary treatments; A: basal diet without rumen-protected fat (RPF), B; basal diet with 2% prilled fat, C; basal diet with 2% calcium salt and D; basal diet with 2% canola oil. At the end of the experiment four out of five animals from each group were slaughtered. The testes were excised for metabolites and gene expression stud-ies. The genes tested were associated to testes development and spermatogenesis (ODF1, SERPINA10, CatSper4, AdipoR2 and DAZL). Feeding RPF with calcium salt (Treatment C) has resulted in the up-regulation more than two folds in all reproductive genes. There were metabolites changes occurred between the groups and identified 44 important putative metabolites present in the testes. In conclu-sion, feeding of RPF to the animals as a source of energy has up-regulated the genes and identified the metabolites involve in the male reproductive tissues and activities.}, issn = {2460-6278}, pages = {76--88} doi = {10.14710/jitaa.48.2.76-88}, url = {https://ejournal.undip.ac.id/index.php/jitaa/article/view/52455} }
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