DOI: https://doi.org/10.14710/jitaa.48.2.76-88

Effects of rumen-protected fat on changes of metabolites and reproductive genes in testes of Malin rams

A. Ismail  -  Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Malaysia
A. I. Muhammad  -  Department of Animal Science, Faculty of Agriculture, Federal University Dutse, Nigeria
L. T. Kee  -  Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Malaysia
L. T. Chwen  -  Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Malaysia
*A. A. Samsudin  -  Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Malaysia
Open Access Copyright (c) 2023 Journal of the Indonesian Tropical Animal Agriculture
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract
The effects of RPF on metabolites and reproductive genes in testes of Malin ram was investigated. Twenty Malin rams (36.6kg ± 5.57 kg of bodyweight), were subjected to four dietary treatments; A: basal diet without rumen-protected fat (RPF), B; basal diet with 2% prilled fat, C; basal diet with 2% calcium salt and D; basal diet with 2% canola oil. At the end of the experiment four out of five animals from each group were slaughtered. The testes were excised for metabolites and gene expression stud-ies. The genes tested were associated to testes development and spermatogenesis (ODF1, SERPINA10, CatSper4, AdipoR2 and DAZL). Feeding RPF with calcium salt (Treatment C) has resulted in the up-regulation more than two folds in all reproductive genes. There were metabolites changes occurred between the groups and identified 44 important putative metabolites present in the testes. In conclu-sion, feeding of RPF to the animals as a source of energy has up-regulated the genes and identified the metabolites involve in the male reproductive tissues and activities.

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Keywords: Energy; Fat; Gene expression; Metabolites; Sheep
Funding: The Government of Malaysia; Universiti Putra Malaysia

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