skip to main content

THE GENETIC DIVERSITY OF KEJOBONG GOAT BASED ON CYTOCHROME B GENE

*J. Jiyanto  -  Faculty of Animal and Agricultural Sciences, Diponegoro University,Tembalang Campus, Semarang 50275, Indonesia
S. Sutopo  -  Faculty of Animal and Agricultural Sciences, Diponegoro University,Tembalang Campus, Semarang 50275, Indonesia
E. Kurnianto  -  Faculty of Animal and Agricultural Sciences, Diponegoro University,Tembalang Campus, Semarang 50275, Indonesia

Citation Format:
Abstract
The purpose of this research was to identify Kejobong goat based on Cytochrome b gene variationby Polymerase Chain Reaction (PCR)-Sequencing technique and to analyze the genetic relationship byusing phylogenetic tree. Materials used were blood sample of 12 heads of Kejobong goat fromKaligondang, Pengadegan, Kejobong, and Bukateja Districts. The steps of this research included DNAextraction, PCR, electrophoresis and DNA sequencing. Forward primer 5'-tggaatctaaccatgaccaatg-3 'andreverse 3'-ggctattctccttttctggttt-5' were used in PCR process. The results of sequences were thenanalyzed by ClustalW in the MEGA 5. The result of this study showed that DNA could be extractedwell. The good results obtained from sequenced for about 779 bp at the nucleotide of 238 to 1016 inCytochrome b. A total of 7 codons diverse sites were detected (sites of 103; 122; 165; 167; 198; 215 and284) at Kejobong goats. There was change in amino acid at 2 sites, those were at 165 sites (*) replacedby (W) in K1 and K2 samples, and at 215 sites (T) replaced by (A) in all samples of Kejobong goats.Kejobong goats at 4 Districts had low genetic distance (0.000-0.001). The analysis of Cytochrome bgene of Kejobong goats originated from different area showed the high similarity and a close geneticrelationship.
Fulltext View|Download
Keywords: Cytochrome b; kambing Kejobong; nukleotida; asam amino; hubungan genetik

Article Metrics:

Last update:

  1. Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences

    Muhammad Fajar Amrullah, Budi Utomo, Suzanita Utama, Tri Wahyu Suprayogi, Tita Damayanti Lestari, Tjuk Imam Restiadi, Ristaqul Husna Belgania. Jurnal Medik Veteriner, 6 (1), 2023. doi: 10.20473/jmv.vol6.iss1.2023.125-131
  2. Genetic diversity of goats in East Java through analysis of the cytochrome B and cytochrome oxidase I genes in mitochondrial DNA

    S. Suyadi, I.L. Murtika, T.E. Susilorini, W.A. Septian, F. Saputra, A. Furqon. Small Ruminant Research, 209 , 2022. doi: 10.1016/j.smallrumres.2022.106659

Last update: 2024-12-04 19:48:45

  1. Amino acid diversity on the basis of cytochrome b gene in kacang and ettawa grade goats

    Lestari D.. Journal of the Indonesian Tropical Animal Agriculture, 42 (3), 2017. doi: 10.14710/jitaa.42.3.135-146
  2. Genetic variation of cytochrome b gene in three local goat breeds in Central Java of Indonesia

    Sutopo S.. Iranian Journal of Applied Animal Science, 8 (4), 2018.
  3. Phylogenetical relationships between Kejobong goat and other goats based on Mt-DNA D-loop sequence analysis

    Lestari D.. Tropical Animal Science Journal, 41 (2), 2018. doi: 10.5398/tasj.2018.41.2.85
  4. Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

    Dela Ayu Lestari, Endang Purbowati, Sutopo Sutopo, Edy Kurnianto. Veterinary World, 11 (8), 2018. doi: 10.14202/vetworld.2018.1196-1202