skip to main content

κ-CASEIN GENE POLYMORPHISMS IN RIVERINE AND SWAMP BUFFALO IN INDONESIA

*A.O. Rini  -  Faculty of Animal Science, Bogor Agricultural University, Jl. Agatis, Bogor 16680, Indonesia
C. Sumantri  -  Faculty of Animal Science, Bogor Agricultural University, Jl. Agatis, Bogor 16680, Indonesia
E. Damayanthi  -  Faculty of Human Ecology, Bogor Agricultural University, Jl. Meranti, Bogor 16680, Indonesia

Citation Format:
Abstract
Kappa-casein (κ-casein) gene is known as a gene that plays a role in controlling milk protein andalso play a crucial role in the coagulation and curdling of milk. This study was aimed to identifypolymorphisms of the κ-Casein gene of local buffaloes in Indonesia. A total number of 40 heads ofriverine buffalo and 250 heads of swamp buffalo. This study used PCR-RFLP method, whichamplification of the κ-Casein gene resulted an amplicon with length of 157 bp, located in exon 4. Theamplified fragment were digested with EcoRV restriction enzyme, which cut the κ-Casein gene in exon4 at nucleotides of GAT|ATC, revealed the presence of one polymorphism at the base position of 23 bpthat occurs with a substitution of Ile (ATC) of the T genetic variant into Thr (ACC) of the C geneticvariant. Genotyping κ-Casein gene in riverine buffalo produced two types of allele, namely C allele (157bp) and T allele (136 and 21 bp). These two alleles resulted in three types of genotypes, namely CC, CT,and TT. Frequency of the C allele was dominant to T allele. κ-Casein gene in swamp buffalo wasmonomorphic with one allele, namely C allele. Heterozygosity value of riverine and swamp buffaloeswere low. PIC value in riverine and swamp buffalo ranged 0.000-0.288. Fixation index of κ-Casein genein riverine buffalo was low (Siborong-borong SBBC = -0,0036; Deli Serdang = -0.025), but in swampbuffalo was in fixation. This study showed that κ-Casein|EcoRV were polymorphic in riverine buffaloand monomorphic in swamp buffalo.
Fulltext View|Download
Keywords: Riverine buffalo; swamp buffalo; κ-Casein; EcoRV; PCR-RFLP; polymorphisms

Article Metrics:

Last update:

  1. Biodiversity of Indonesian indigenous buffalo: First review of the status, challenges, and development opportunities

    Peni Wahyu Prihandini, Yuli Arif Tribudi, Dwi Nur Happy Hariyono, Aprilianna Putri Zahara Nafsina Luvita Sari, Lisa Praharani, Eko Handiwirawan, Bess Tiesnamurti, Endang Romjali, Procula Rudlof Matitaputty, Agus Wiyono. Veterinary World, 2023. doi: 10.14202/vetworld.2023.2217-2229

Last update: 2024-11-01 14:05:37

No citation recorded.