DOI: https://doi.org/10.14710/jitaa.38.2.86-91

IDENTIFICATION OF MELANOCORTIN 1 RECEPTOR (MC1R) GENE BASED ON COAT COLOR OF BALI COWS OF KUPANG BY USINGTHE PCR-RFLP METHOD

*A.C. Tabun  -  Kupang State Agicultural Polytechnic,Jl. AdisuciptoPenfui, Kupang NTT 1152, Indonesia
T. Hartatik  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
S. Sumadi  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia

Citation Format:
Abstract
The objective of this study was to identify the Melanocortin 1 Receptor (MC1R) gene in sorrel-,black- and white-Bali cows originated from Kupang-East Nusa Tenggara. Blood samples were takenfrom 46 cows consisted of of 17, 18 and 8 of sorrel, black and 8 heads, respectively, and 3 heads of bull.The PCR-RFLP method was performed to identify the gene. Amplification of PCR gene MC1R ofKupang's Bali Cattle (KBC) was 296 bp. The digestion by using MspI enzyme showed that there wereEE genotype (169, 136 bp) in Bali bull, sorrel and white cows. Two 2 genotypes, those were EE (169,136 bp) and Ee (296, 169, 136 bp) were found in black Bali cow. The frequency of E alele of KBC was0.99 (99%) and e alele was 0.01 (1%). MC1R gene in KBC was monomorphic and the it can be used asthe marker of the coat color in the population.
Fulltext View|Download
Keywords: Melanocortin 1 Receptor. Bali cow. coat color. amplification. monomorphic

Article Metrics:

Article Info
Section: Articles
Language : EN
Recent articles
THE SOAKING EFFECT ON DIFFERENT HYDROCLORIDE ACID LEVEL AND SOAKING TIME ON PH, SWELLING PERCENTAGE AND COLLAGEN YIELD OF CHICKEN SHANK BONE ASSESSMENT OF METHANE ESTIMATION FROM VOLATILE FATTY ACID STOICHIOMETRY IN THE RUMEN IN VITRO DETERMINATION OF PREDICTION EQUATIONS TO ESTIMATE BODY CONDITION SCORE FROM BODY SIZE AND TESTICULAR TRAITS OF YANKASA RAMS More recent articles
Most cited articles
AMINO ACID PROFILE OF EARTHWORM AND EARTHWORM MEAL (Lumbricus rubellus) FOR ANIMAL FEEDSTUFF ESTIMATION OF SELECTION ACCURACY AND RESPONSES OF THE PRODUCTION CHARACTERISTICS USING DIFFERENT SELECTION INTENSITY IN MAGELANG DUCK Modeling for determining the superiority of Holstein bulls as frozen semen producer and genetic source for milk production Effect of tomato (Solanum lycopersicum L.) protein on carcass and meat quality of kampong chicken TOTAL NON-STRUCTURAL CARBOHYDRATE (TNC) OF THREE CULTIVARS OF NAPIER GRASS (Pennisetum purpureum) AT VEGETATIVE AND REPRODUCTIVE PHASE More cited articles

Last update:

  1. Sequence Analysis and Identification of Allele Distribution of Melanocortin 1 Receptor (MC1R) Gene in Indonesian Cattle (Bos sondaicus×Bos indicus)

    Tety Hartatik. Asian Journal of Animal Sciences, 11 (1), 2016. doi: 10.3923/ajas.2017.40.46

  2. Lack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes

    Jakaria Jakaria, Kholijah Kholijah, Sri Darwati, Qonita Rahman, Winni Liani Daulay, Ikhsan Suhendro, I. Made Londra, Mokhamad Fakhrul Ulum, Ronny Rachman Noor. Veterinary World, 2023. doi: 10.14202/vetworld.2023.1312-1318

Last update: 2024-04-24 04:41:07

No citation recorded.