NUCLEAR MATURATION RATE OF SHEEP OOCYTES IN VITRO: EFFECT OF STORAGE DURATION AND OVARY TEMPERATURE

Abstract
The present study was conducted to investigate the effects of duration and temperature storageof sheep ovaries on nuclear maturation in vitro of oocytes obtained from stored ovaries. Ovaries werecollected from slaughterhouse and stored in physiological saline for 2-4 h, 5-7 h and 8-10 h at varioustemperatures (27-28°, 36-37° and 4°C). Cumulus oocyte complexes (COCs) were collected with slicingmethod from each group and matured in vitro for 26 h. There was no difference between the proportionsof oocytes maturation to metaphase II when collected from ovaries stored at 27-28°C and 36-37°C for 2-7 h of slaughter. However, the percentages of oocytes from ovaries stored at 4°C were significantlylower (P<0.05) than those stored at higher temperature (69.23%, 70.83% and 45.65%, for 27-28, 36-37and 4°C at 2-4 h, respectively; 59.61%, 64,58% and 36.36% for 27-28, 36-37 and 4°C at 5-7 h,respectively). The proportion of oocytes meiosis to metaphase II (MII) were significantly (P<0.05)decreased when the ovaries stored at 27-28 and 36-37°C for 8-10 h, but not in oocytes stored at 4°C(24.37%, 7.84% and 45.23%, respectively). The maturation rate of oocytes from ovary stored at 4°C washigher than those collected from ovaries stored at higher temperature (P<0.05). This finding indicated that the storage of ovaries at 4°C for 8-10 h is effective for maintaining the development competence ofsheep oocyte better than storage at higher temperature.
Keywords: ovary; in vitro maturation; sheep oocyte; temperature; time storage
Article Metrics:
Related articles
Cholesterol Assimilation of Saccharomyces cerevisiae B-18 isolated from gastrointestinal tract of Javanese duck
EVALUATION OF NUTRITIVE VALUE AND IN VITRO METHANE PRODUCTION OF FEEDSTUFFS FROM AGRICULTURAL AND FOOD INDUSTRY BY-PRODUCTS
FERMENTATION QUALITY OF KING GRASS SILAGE TREATED WITH LIQUID OR DRIED INOCULANT OF LACTIC ACID BACTERIA
IN VITRO EVALUATION OF CONCENTRATE FEED CONTAINING Indigofera zollingeriana IN GOAT
In vitro rumen fermentability kinetics of parboiled rice bran
EFFECT OF WATER EXTRACT OF PLANTS CONTAINING TANNIN ON IN VITRO METHAGONESIS AND FERMENTATION CHARACTERISTICS OF THE GRASS Pennisetum purpureophoides
Last update: 2021-03-01 02:53:51
-
Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
Animals, 10 (12), 2020. doi: 10.3390/ani10122414
Last update: 2021-03-01 02:53:52
-
Cellular and Molecular Events that Occur in the Oocyte during Prolonged Ovarian Storage in Sheep
Animals, 10 (12), 2020. doi: 10.3390/ani10122414