IDENTIFICATION OF UTERIN MILK PROTEIN (UTMT) GENE IN BALI CATTLE USING DIRECT SEQUENCING

*J. Jakaria -  Faculty of Animal Science, Bogor Agricultural University Jl. Agatis, Darmaga Campus, Bogor 16680, Indonesia
F. Saputra -  Faculty of Animal Science, Bogor Agricultural University Jl. Agatis, Darmaga Campus, Bogor 16680, Indonesia
K. A. Paramitasari -  Faculty of Animal Science, Bogor Agricultural University Jl. Agatis, Darmaga Campus, Bogor 16680, Indonesia
P. P. Agung -  Research Center for Biotechnology, Indonesian Institute of Science Jl. Raya Bogor KM 46, Cibinong 16911, Indonesia
M. Maskur -  Faculty of Animal Science, Nataram University Jl. Majapahit No 62, Mataram 83125, Lombok, Indonesia
Received: 22 Dec 2015; Published: 1 Mar 2016.
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Abstract
The objective of this research was to identify diversity of exon 5 UTMP gene fragment in Bali cattle using direct sequencing. The total 60 blood samples of Bali Cattle derived from BPTU Bali in Bali siland (20 heads), BPTU Serading in Sumbawa island (20 heads) and Village Breeding Center in Barru District South Sulawesi (20 heads) were used to evaluate their genetic diversity at exon 5 UTMP gene. The forward and reverse data sequences were analyzed using Bioedit program and alignment analysis was carried out using MEGA5 program. Meanwhile haplotype analysis was performed by DnaSPv5 program. The result showed that partial sequences in exon 5 UTMP gene had 16 haplotypes with the highest number of haplotypes ware found in VBC Barru district South Sulawesi (8 haplotypes). Moreover, the highest average of haplotype (h) and nucleotide (p) diversity were found in VBC Barru district South Sulawesi were 0.7949 and 0.0016, respectively. In addition, minisatellite insersion was found in exon 5 UTMP gene fragment on Bali cattle which are consist of 5'-CCA GTC ATG AAG AAG GCA GAG GTC GTC GTG CCG GCG AAA-3'. According to our results, haplotype and minisatellite variation in exon 5 UTMP gene fragment can be used as a candidate genetic marker specific for reproductive trait in the Bali cattle and for its strategy breeding program in the future.
Keywords
UTMP gene; diversity; haplotype; Bali cattle

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