The Growth of Botryococcus braunii Microalgae as a Lipid Producer in a Mixed Medium of Coconut Water and Seawater

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Submitted: 04-03-2016
Published: 15-10-2015
Section: Articles
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Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions for bioenergy. Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be substituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using hemocytometer. The results showed that 2.5% coconut water substitution exhibited the best growth rate and lipid production, i.e. the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control.
  1. Bintoro Rudi Saputro 
    Biology Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
  2. Endang Kusdiyantini 
    Biology Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
  3. Hermin Pancasakti Kusumaningrum 
    Biology Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia