Muhammad Nursid
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Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Indonesia
Bambang Cahyono
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Organic Chemistry Laboratory, Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Semarang Jalan Prof. Soedarto, Tembalang, Semarang 50275, Telepon +62-24-7474754, Indonesia
Abstract
ABSTRACT---One of the Indonesian marine natural resources, brown alga of Turbinaria decurrens, used in pharmacy in order of carotenoid pigment as antioxidant. We interested in analysis B-caroten in Turbinaria decurrens and antioxidant and anti-tumor activity. The method that is use to divide carotenoid is the hierarchy maseration method using n-hexane, ethyl acetate and methanol and then using chromatography column. To analyze the qualitative carotenoid uses HPLC by Crc column and eluen
methanol-acetonitrile (3:I). Meanwhile, the test of bioactivity carotene uses radical DPPH (1I'difenil-2-
pibilhidrozil) to test of antioxidant qnd sitotoksik asscy with MTT [3,(4,5-dimethylthiazol-2yl)-2,5-
diphenyltetrcaolium bromideJ for HeLa tumor cells to test of anti-tumor. The result of this research is B -
caroten thqt is in qctract has 0.00387%. Moreover, bioacttvity test shows that B -caroten fraction does not
active to neutralize of DPPHfree radical than ascorbic aci{ but it shows the acttvity to kill HeLa tumor cells.
Keywords : Turblnaria decunens, Carolenoid, Antioksidan, Antitumor