Biogas production potential from a native beverage vinasse of Mexico

Abumalé Cruz-Salomón



This work presents the results of the physicochemical characterization and evaluates the potential of vinasse (a wastewater obtained from local beverage of Chiapas, Mexico) as nutrient source for biogas production by anaerobic digestion in an Upflow Anaerobic Sludge Blanket (UASB) bioreactor. This wastewater contains high level of organic matter, 120.2 ± 18.4 g O2/L of chemical organic demand (COD), 0.77 biodegradability index (BI), and a ratio 21:1 of C/N. The UASB bioreactor had a working volume of 4 L, at 32 oC, 10 days of hydraulic retention time (HRT). The COD removal was of 91 %, the production of methane was 1860 mL/day and 264.83 mL CH4/gCOD of biochemical methane potential (BMP). As such, the UASB bioreactor fed with vinasse can be sustainable alternative for the bioenergy production from wastewater, shifting the paradigm of wastewater management from ‘treatment and disposal’ to ‘beneficial utilization’ as well as ‘profitable endeavor’.  


anaerobic digestion; UASB; vinasse; COD; biodegradability; BMP

Full Text:



Benitez, F.J., Real, F.J., Acero, J.L., Garcia, J., Sanchez, M., 2003. Kinetics of the ozonation and aerobic biodegradation of wine vinasses in discontinuous and continuous processes. J. Hazard. Mater. 101 (2) (2003) 203–218.

Benke, M., A. Mermut and H. Shariatmadari: Retention of dissolved organic carbon from vinasse by a tropical soil, kaolinite and Fe-oxides. Geod. 91(1999) 47-63.

V. Robles-González, J. Galíndez-Mayer, N. Rinderknecht-Seijas, M. Poggi- Varaldo. Treatment of mezcal vinasses: A review, Journal of Biotechnology 157 (2012) 524–546.

P.C., Sangave, P.R. Gogate, A.B. Pandit. Ultrasound and ozone assisted biological degradation of thermally pretreated and anaerobically pretreated distillery wastewater. Chemosphere 68(1) (2007) 42–50.

A. Jiménez. Kinetic analysis of the anaerobic digestion of untreated vinasses and vinasses previously treated with Penicillium decumbs. Journal of Enviromental Managment 80, 4 (2006) 303–310.

E. Houbron, M.E. Sandoval-Rojas and A.F. Hernández-Muñoz. Treatment of vinasse in an anaerobic reverse fluidized bed reactor. Rev. Int. Contam. Ambie. 32 (3) (2016) 255-266.

INEGI. (2012). Statistical Yearbook of Chiapas, Internet consultation on April 7, 2015:

APHA (American Public Health Association), Standard Methods for the Examination of Water and Wastewater, American Public Health Association/American Water Works Association/Water Environment Federation, Washington, DC, USA, 20th edition, 2005.

A. Walkley, I. Black. An examination of the Degtjareff method for determining organic carbon in soils: Effect of variations in digestion conditions and of inorganic soil constituents, Soil Science 63 (2006) 251-263.

V.I. Singleton, R. Orthofer, R.M. Iamuela-Raventos. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299 (1999)152-178.

D. Bello-Gil, E. Carrera-Bocourt, Y. Díaz-Maqueira. (2006). Determination of total reducing sugars in mixed sugarcane juice using the dinitrosalicylic acid method 3.5, ICIDCA. Derivatives on sugarcane 40(2) (2006) 45-50.

E. Foresti, L. Florencio, A. Van Haandel, M. Zaiat, P. Cavalcanti. Fundaments of anaerobic treatment. Chapter 2. In: Treatment of sewage by anaerobic process and disposition controlled soil. PROSAB, Brazil (1999) 436.

C. Chernicharo. Principles of biological wastewater treatment. Federal University of Minas Gerais. Vol V. Brazil (1997).

P. Torres-Lozada and Pérez, A. Specific methanogenic activity: a control tool and system optimization of anaerobic wastewater treatment. Engineering of Natural Resources and Environment, 9 (2010) 5 -14.

F. Raposo, V. Fernández-Cegrí, M.A. De la Rubia, R. Borja, F. Béline, C. Cavinato, G. Demirer, B. Fernández, M. Fernández-Polanco, J.C. Frigon, R. Ganesh, P. Kaparaju, J. Koubova, R.Méndez, G. Menin, A. Peene, P. Scherer, M. Torrijos, H. Uellendahl, I. Wierinck, V. de Wilde. Biochemical methane potential (BMP) of solid organic substrates: evaluation of anaerobic biodegradability using data from an international inter-laboratory study. J Chem Technol Biotechnol, 86(2011) 1088–1098.

Harris, P. Carbon dioxide content of biogas by Syringr Protocol. Published in Beginners Guide to Biogas, The University of Andalaide, Australia (2010).

S. Zárate-Jiménez, S. Pérez-Fabiel, G. Yáñez-Ocampo, J. Chanona-Soto. Anaerobic digestion of fruit and vegetable waste by mesophilic via laboratory scale. Revista AIDIS 7(2) (2014) 115 – 124.

A. Punal, M. Trevisan, A. Rozzi, J. Lema. Influence of C:N Ratio on the Start-up of Upflow Anaerobic Filter Reactors. Water Res. 34 (2000) 2614–2619.

P. Aguirre. Removal mechanisms of organic matter and nutrients in subsurface flow constructed wetlands. New criteria for the design and operation of constructed wetlands. CPET. Barcelona. (2004)17-29.

A. Cruz-Salomón, R. Meza-Gordillo, A. Rosales-Quintero, C. Ventura-Canseco, S. Lagunas-Rivera, J. Carrasco-Cervantes. Biogas production from a native beverage vinasse using a modified UASB biorreactor. Fuel (2016) DOI: 10.1016/j.fuel.2016.11.046

M. Varnero, J. Arellano. Rational use of organic waste. Ministry of Agriculture (FIA). University of Chile. Faculty of Agricultural and Forestry Sciences, Technical report. Santiago, Chile. 98 (1991).

E. Taiganides. Biogas: energy recovery of animal excrement. Zootecnia 35 (1980) 2-12

P. Smith, F. Bordeaux, A. Wilkie, J. Yang, D. Boone, R. Mah, D. Chynoweth, D. Jerger. Microbial aspects of biogas production. In W. Smith, J. Frank (eds). Methane from biomass: a Systems Approach: (1988) 335-353. London: Elsevier Applied Science Publishers.

S. Rizem, G. Lettinga (1988). Methanogenic toxicity of phenolic and lipidic compounds. In: Proceedings of the 4th Seminar of Anaerobic Wastewater Treatment. University of Valladolid. Valladolid, Spain (1988) 83-110.

M. Mussati, P. Aguirre, N. Scenna. Modeling, simulation and optimization of chemical processes. Chapter XVIII: Modeling of anaerobic digestion process in simple reactors. Ed. Nicolás J. Scenna; National Technology University-CONICET. Santa Fé, Argentina (1999) 697-739

J. Kim, C. Park, T.H. Kim, M. Lee, S. Kim, S.W. Kim and J. Lee. Effects of various pretreatments for en- hanced anaerobic digestion with waste activated sludge. Journal of Bioscience and Bioengineering, 95(2003) 271-275.

D.H. Lee, S.K. Behera, J. Kim and H.S. Park. Methane production potential of leachate generated from Korean food waste recycling facilities: a lab scale study. Waste Management, 29 (2009) 876-882.

J.B. van Lier. Biological wastewater treatment: Principles Modelling and Desing. Edited by M. Henze, M.C.M. van Loosdrecht, G.A. Ekama and Brdjanovic. ISBN: 9781843391883. Published by IWA Publishing, London, UK. (2008).

WHO, Guideline for Discharge of Industrial Effluent Characteristics, vol. 3, WHO, Geneva, 1995, pp. 231–236.

J. Dolfing. Activity measurements as a tool to characterize the microbial composition of methanogenic environments. Journal of Microbiological Methods 4 (1985) 1-12;

J. Field. Operating parameters of anaerobic sludge blanket upflow. Manual Startup and operation of systems with upflow sludge blanket – UASB. Universidad del Valle, CVC. Wageningen Agricultural University (1987).

S. Guiot. A structured model of the anaerobic granule consortium. Water Science Technology 25 (1992) 7, 1-10.

L. Janke, A. Leite, M. Nikolausz, T. Schmidt, J. Liebetrau, M. Nelles and W. Stinner. Biogas production from sugarcane waste: assessment on kinetic challenges for process designing. Int. J. Mol. Sci. 16(2015) 20685-20703.

A.F. Leite, L. Janke, H. Harms, J.W. Zang, W.A. Fonseca-Zang, W. Stinner, M. Nikolausz. Assessment of the variations in characteristics and methane potential of major waste products from the Brazilian bioethanol industry along an operating season. Energy Fuels 29 (2015) 4022–4029.

B.S. Moraes, M. Zaiat, A. Bonomi. Anaerobic digestion of vinasse from sugarcane ethanol production in Brazil: Challenges and perspectives. Renewable and Sustainable Energy Reviews 44 (2015) 888–903.

D. Deublein and A. Steinhauser. Biogas from Waste and Renewable Resources: General aspects of the recovery of biomass in the future. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim ISBN: 978-3-527-31841-4. (2008).

Published by Waste Resources Research Center (WRRC), Diponegoro University - Indonesia
WasTech by is licensed under Creative Commons Attribution-ShareAlike 4.0.