BibTex Citation Data :
@article{BULOMA20896, author = {Diyah Putri Ambarwati and Ervia Yudiati and Endang Supriyantini and Lilik Maslukah}, title = {Pola Pertumbuhan, Biomassa Dan Kandungan Protein Kasar Kultur Skeletonema costatum Skala Massal Dengan Konsentrasi Kalium Nitrat Berbeda}, journal = {Buletin Oseanografi Marina}, volume = {7}, number = {2}, year = {2018}, keywords = {Skeletonema; KNO3; pertumbuhan; biomassa; protein; KNO3; growth; biomass; protein}, abstract = { Mikroalga Skeletonema costatum merupakan pakan alami yang mengandung nutrisi yang diperlukan untuk budidaya perikanan. Modifikasi media kultur merupakan salah satu upaya optimalisasi produktivitas sekaligus meningkatkan kadar proteinnya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian KNO 3 dengan berbagai konsentrasi terhadap pola pertumbuhan, biomassa dan kandungan protein kasar. Metode penelitian yang digunakan adalah metode eksperimen Rancangan Acak Lengkap, dengan lima konsentrasi KNO 3 (A: 25; B:50; C:75; D:100 dan E:125 ppm) yang diulang sebanyak tiga kali. Inokulum awal adalah satu ton,10 3 sel/mL. Saat mencapai fase stasioner, kultur massal S. costatum dipanen dan dikeringkan. Kandungan protein kasar dianalisis menggunakan metode Kjeldahl. Hasil penelitian terhadap kepadatan menunjukkan bahwa perlakuan terbaik dicapai pada konsentrasi KNO 3 125 ppm (498,88. 10 3 sel/mL) diikuti D (316,94. 10³ sel/mL), C (313,81.10³ sel/mL), B (246,56. 10³ sel/mL) dan A (195,60. 10³ sel/mL). Terdapat perbedaan fase stasioner pada masing-masing perlakuan yaitu A:32; B:28; C: 37; D:32; E:36 jam. Biomass rata-rata yang dihasilkan pada tiap perlakuan sesuai dengan pola kepadatan sel yaitu E: 8,60, D:7,85, C:6,43, B:5,91 dan A:5,44 g. Analisa terhadap kandungan protein kasar menunjukkan tidak adanya perbedaan antar perlakuan (P>0,05). Pemberian KNO 3 terbukti dapat meningkatkan kepadatan sel dan berpengaruh terhadap pola pertumbuhan dan biomassa, namun kandungan protein kasar tidak meningkat. Growth, Biomass and Rough Protein Content of Skeletonema costatum Mass Scale with Different Concentration of Potassium Nitrate (KNO 3 ) Skeletonema costatum is natural microalgae which rich in nutrient. An effort to optimize the protein content was done by modifying the culture media. This research is aimed to find out the effect of various concentration of KNO3 addition to the growth pattern, biomass and crude protein content. The Completely Randomized Design with five treatment KNO3 (A: 25; B:50; C:75; D:100 dan E:125 ppm) in three replication were applied. The starting innoculum was one tonne, 10 3 cel/mL. The microalgae was harvested at stationary phase and the dried. The crude protein was analised by Kjeldahl methods. The results of cell density showed that the best concentration was E (498,88. 10 3 cells/mL), D (316,94. 10³ cells/mL), C (313,81.10³ cells/mL), B (246,56. 10³ cells/mL) and A (195,60. 10³ cells/mL) respectively. The stationary phase in every treatment was reached at different time A:32; B:28; C: 37; D:32; E:36 hr. The average biomass were at similar pattern to the cell density (E:8,60, D:7,85, C:6,43, B:5,91 and A:5,44 g). On the other hand, the crude protein content were not significantly different (P>0,05). It is concluded that the KNO 3 addition enhanced the cell density, growth pattern, biomass. The protein content was remain similar. }, issn = {2550-0015}, pages = {75--80} doi = {10.14710/buloma.v7i2.20896}, url = {https://ejournal.undip.ac.id/index.php/buloma/article/view/20896} }
Refworks Citation Data :
Mikroalga Skeletonema costatum merupakan pakan alami yang mengandung nutrisi yang diperlukan untuk budidaya perikanan. Modifikasi media kultur merupakan salah satu upaya optimalisasi produktivitas sekaligus meningkatkan kadar proteinnya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian KNO3 dengan berbagai konsentrasi terhadap pola pertumbuhan, biomassa dan kandungan protein kasar. Metode penelitian yang digunakan adalah metode eksperimen Rancangan Acak Lengkap, dengan lima konsentrasi KNO3 (A: 25; B:50; C:75; D:100 dan E:125 ppm) yang diulang sebanyak tiga kali. Inokulum awal adalah satu ton,103 sel/mL. Saat mencapai fase stasioner, kultur massal S. costatum dipanen dan dikeringkan. Kandungan protein kasar dianalisis menggunakan metode Kjeldahl. Hasil penelitian terhadap kepadatan menunjukkan bahwa perlakuan terbaik dicapai pada konsentrasi KNO3 125 ppm (498,88. 103 sel/mL) diikuti D (316,94. 10³ sel/mL), C (313,81.10³ sel/mL), B (246,56. 10³ sel/mL) dan A (195,60. 10³ sel/mL). Terdapat perbedaan fase stasioner pada masing-masing perlakuan yaitu A:32; B:28; C: 37; D:32; E:36 jam. Biomass rata-rata yang dihasilkan pada tiap perlakuan sesuai dengan pola kepadatan sel yaitu E: 8,60, D:7,85, C:6,43, B:5,91 dan A:5,44 g. Analisa terhadap kandungan protein kasar menunjukkan tidak adanya perbedaan antar perlakuan (P>0,05). Pemberian KNO3 terbukti dapat meningkatkan kepadatan sel dan berpengaruh terhadap pola pertumbuhan dan biomassa, namun kandungan protein kasar tidak meningkat.
Growth, Biomass and Rough Protein Content of Skeletonema costatum Mass Scale with Different Concentration of Potassium Nitrate (KNO3)
Skeletonema costatum is natural microalgae which rich in nutrient. An effort to optimize the protein content was done by modifying the culture media. This research is aimed to find out the effect of various concentration of KNO3 addition to the growth pattern, biomass and crude protein content. The Completely Randomized Design with five treatment KNO3 (A: 25; B:50; C:75; D:100 dan E:125 ppm) in three replication were applied. The starting innoculum was one tonne, 103 cel/mL. The microalgae was harvested at stationary phase and the dried. The crude protein was analised by Kjeldahl methods. The results of cell density showed that the best concentration was E (498,88. 103 cells/mL), D (316,94. 10³ cells/mL), C (313,81.10³ cells/mL), B (246,56. 10³ cells/mL) and A (195,60. 10³ cells/mL) respectively. The stationary phase in every treatment was reached at different time A:32; B:28; C: 37; D:32; E:36 hr. The average biomass were at similar pattern to the cell density (E:8,60, D:7,85, C:6,43, B:5,91 and A:5,44 g). On the other hand, the crude protein content were not significantly different (P>0,05). It is concluded that the KNO3 addition enhanced the cell density, growth pattern, biomass. The protein content was remain similar.
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