1Satya Wacana Christian University, Indonesia
2Marine Science Department, Indonesia
3Universitas Diponegoro, Indonesia
4 Faculty of Fisheries and Marine Science Diponegoro University, Indonesia
5 Graduate School of Biology Satya Wacana Christian University, Indonesia
BibTex Citation Data :
@article{IK.IJMS13417, author = {Iqna Abfa and Ocky Radjasa and A Susanto and Handung Nuryadi and Ferry Karwur}, title = {Exploration, Isolation, and Identification of Carotenoid from Bacterial Symbiont of Sponge Callyspongia vaginalis}, journal = {ILMU KELAUTAN: Indonesian Journal of Marine Sciences}, volume = {22}, number = {2}, year = {2017}, keywords = {}, abstract = { During the past two decades research on marine bacteria has highlighted the tremendous potential of symbiotic-microorganisms as a source of bioactive secondary. One of the potential of the bacterial symbionts is producing a natural pigment, and these organisms can be used as a sustainable source of natural pigments. Carotenoid is one of the most important pigments that has important roles in physiological and molecular processes of microorganisms, as well as for human health. The objective of this study is to analyze carotenoid pigments from marine bacterial symbionts from sponge and to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by a UV-VIS spectrophotometer and High Performance Liquid Chromatography (HPLC). Molecular bacterial identification was performed based on 16S rDNA sequence . The isolation of bacterial symbionts from C. vaginalison Zobell 2216E medium resulted in one bacterium, CB-SP5, positively synthesized carotenoids. By reverse phase HPLC analysis, the carotenoid pigments in the bacterial symbionts were identified as diadinoxanthin, fucoxanthin, neoxanthin, dinoxanthin, anddiadinochrome. CB-SP5 shared the highest level of 16S rDNA gene sequence similarity with Psychrobacter celer (99%). Keywords : carotenoid, sponge, bacterial symbiont, 16S rDNA. }, issn = {2406-7598}, pages = {49--58} doi = {10.14710/ik.ijms.22.2.49-58}, url = {https://ejournal.undip.ac.id/index.php/ijms/article/view/13417} }
Refworks Citation Data :
During the past two decades research on marine bacteria has highlighted the tremendous potential of symbiotic-microorganisms as a source of bioactive secondary. One of the potential of the bacterial symbionts is producing a natural pigment, and these organisms can be used as a sustainable source of natural pigments. Carotenoid is one of the most important pigments that has important roles in physiological and molecular processes of microorganisms, as well as for human health. The objective of this study is to analyze carotenoid pigments from marine bacterial symbionts from sponge and to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by a UV-VIS spectrophotometer and High Performance Liquid Chromatography (HPLC). Molecular bacterial identification was performed based on 16S rDNA sequence. The isolation of bacterial symbionts from C. vaginalison Zobell 2216E medium resulted in one bacterium, CB-SP5, positively synthesized carotenoids. By reverse phase HPLC analysis, the carotenoid pigments in the bacterial symbionts were identified as diadinoxanthin, fucoxanthin, neoxanthin, dinoxanthin, anddiadinochrome. CB-SP5 shared the highest level of 16S rDNA gene sequence similarity with Psychrobacter celer (99%).
Keywords : carotenoid, sponge, bacterial symbiont, 16S rDNA.
Article Metrics:
Last update:
Time for a Change! A Spotlight on Many Neglected Facets of Sponge Microbial Biotechnology
Oxidative Stress and Marine Carotenoids: Application by Using Nanoformulations
Last update: 2024-12-25 07:18:48
Copy this form and after filling it, please send it to ijms@live.undip.ac.id:
COPYRIGHT TRANSFER STATEMENT
When this article is accepted for publication, its copyright is transferred to ILMU KELAUTAN Indonesian Journal of Marine Sciences, UNDIP. The copyright transfer covers the non exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature.
The author warrants that this article is original and that the author has full power to publish. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors. In regard to all kind of plagiarism in this manuscript, if any, only the author(s) will take full responsibility. If the article is based on or part of student’s skripsi, thesis or dissertation, the student needs to sign as his/her agreement that his/her works is going to be published.
Title of article :...........................................................................................................................Name of Author(s) :...........................................................................................................................Author’s signature :...........................................................................................................................Date :...........................................................................................................................
View My Stats
