Skrining Bakteri yang Berasosiasi dengan Spons Jaspis sp. Sebagai Penghasil Senyawa Antimikroba

*Hermawaty Abubakar  -  Jurusan Biologi FMIPA Universitas Negeri Papua, Jl. Gunung Salju, Amban, Manokwari 98314, Indonesia
Aris Tri Wahyudi  -  Departemen Biologi FMIPA IPB, Jl. Agatis, Kampus IPB Darmaga Bogor, Indonesia
Munti Yuhana  -  Departemen Budidaya Perikanan IPB, Jl. Agatis, Kampus IPB Darmaga Bogor, Indonesia
Received: 11 Feb 2012; Published: 12 Feb 2012.
Open Access

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Article Info
Section: Research Articles
Language: ID
Statistics: 6029 9534
Abstract

Organisme bentik laut seperti spons, seringkali hidup berasosiasi dengan bakteri yang menghasilkan senyawa antimikroba.  Penelitian  ini  bertujuan  untuk  mengetahui  kemapuan  antagonis  isolat-isolat  bakteri  yang berasosiasi dengan spons Jaspis sp. terhadap beberapa bakteri patogen, dengan metode skrining secara kualitatif. Sebanyak 32 (45,71%) dan 20 (29,41%) isolat yang berasal dari bagian mesohyl dan permukaan Jaspis sp. menunjukkan kemampuan antimikroba, karena mampu menghambat pertumbuhan Staphylococcus aureus, Vibrio harveyii, Escherichia coli, Pseudomonas aerogenosa, EPEC K-11, Candida albicans, and C. tropicalis. Uji fenotipik dilakukan pada beberapa isolat dengan aktivitas antimikroba terbaik, yaitu SAB E-8, SAB E-33, SAB E-35, SAB E-38, SAB E-40 dan SAB S-43. Hasil pewarnaan Gram menujukkan isolat  SAB E-8, SAB E35, and SAB E-40 adalah Gram negatif, sedangkan isolat SAB E-33, SAB E-38, and SAB S-43 adalah gram positif yang dilanjutkan dengan identifikasi parsial (pengecatan gram dan uji katalase) untuk kelompok Bacillus.

Kata kunci: Bakteri, Asosiasi, Jaspis sp., antimikroba

 

Living benthic marine organisms such as sponges are frequently assosiated with as bacteria that may be produce antimicrobial compounds. This study aims to determine antagonistic of bacterial isolates that associated sponge Jaspis sp., with a qualitative screening method. Screening of bacteria from marine sponge Jaspis sp. which have bility to produce antibacterial subtances was investigated. There are 32 (45,71%) and 20 (29,41%) isolates from mesohyl and surface sponge respectively. Those isolated bacterial showed the antibacterial activity against Staphylococcus aureus, Vibrio harveyii, Escherichia coli, Pseudomonas aerogenosa, EPEC K-11, Candida albicans, and C. tropicalis. However, use of a few additional simple phenotypic tests for those isolate can be used to differentiate among isolates. The simple phenotypic test divided two ways based on staining gram. Gram negative bacteria were desingned SAB E-8, SAB E-35, and SAB E-40 and gram positive bacteria were desingned SAB E-33, SAB E-38, and SAB S-43. Parsial identification that directed to Bacillus was used for positive gram bacteria, involve gram staining, endospora staining and katalase test.

Key words: Bacteria, Assosiation, Jaspis sp, antimicrobe

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