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Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

1Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Indonesia

2Marine Natural Product Laboratory, Centre for Research and Services, Universitas Diponegoro, Indonesia

3Department Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Indonesia

4 Tropical Marine Biodiversity Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Indonesia

5 Department Public Administration, Faculty of Social and Politic Science, Universitas Diponegoro, Indonesia

6 Faculty of Fisheries and Marine Sciences, Universitas Sam Ratulangi, Indonesia

7 Agriculture Faculty, Univeristas Tadulako, Indonesia

8 School of Natural and Environmental Sciences, New Castle University, United Kingdom

9 Centre for Conservation and Restoration Science, Edinburgh Napier University, United Kingdom

10 School of Applied Sciences, Edinburgh Napier University, United Kingdom

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Received: 25 May 2022; Revised: 15 Mar 2023; Accepted: 5 May 2023; Available online: 21 May 2023; Published: 21 May 2023.

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Abstract

Mangroves are complex, unique, and dynamic ecosystems that host organisms that have special physiological adaptations to fluctuations in salinity, temperature, and pH. Gastropods have an important role in the mangrove ecosystem for food cycles and webs by helping to provide nutrients and micro-habitats for microbes.  Micro-fungi isolated from mangrove ecosystems are productive sources of enzymes and bioactive compounds such as antibacterial and antifungal. In this study a explored the enzymatic and anti-vibrio activity of fungi associated with the marine gastropod Cerithidea cingulata and Lottia scabra. collected in three mangrove forests in North Sulawesi, Indonesia.  The fungi associated with the specimens were inoculated on malt extract agar (MEA) using the tapping method. Enzymatic and anti-vibrio assays were conducted applying the plug method. Positive effects of the fungal compounds on Vibrio  were indicated by  clear zones formed around the isolates.  A total of 13 fungal species were isolated from C. cingulata (19MB-C5  and 19MBa-A4) and L. scabra (19MT-07 and 19MT-18).  Most fungal isolates have moderate growth rate. The isolate with highest cellulolytic, proteolytic, and anti-vibriosis activity (Vibrio parahaemolyticus, V. harveyi, V. vulnificus, and V. alginolyticus), derived from C. cingulata 19MB-C5 was closely related to Aspergillus niger, as revealed by molecular analysis using the universal primers ITS1 and ITS4 (similarity value 99%).  A. niger 19MB-C5-3 has potential as a bioremediation and anti-vibrio agent that can be applied in aquaculture.

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Keywords: enzyme; fungi; mangrove; vibriosis

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