Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp

*Sumardi Sumardi -  Jurusan Biologi, FMIPA Universitas Lampung Jl. Sumantri Brojonegoro No. 1 Gedong Meneng Bandar Lampung, Indonesia
Rochmah Agustrina -  Jurusan Biologi, FMIPA Universitas Lampung Jl. Sumantri Brojonegoro No. 1 Gedong Meneng Bandar Lampung, Indonesia
Bambang Irawan -  Jurusan Biologi, FMIPA Universitas Lampung Jl. Sumantri Brojonegoro No. 1 Gedong Meneng Bandar Lampung, Indonesia
Indah Selfiana -  Jurusan Biologi, FMIPA Universitas Lampung Jl. Sumantri Brojonegoro No. 1 Gedong Meneng Bandar Lampung, Indonesia
Received: 19 Feb 2018; Published: 15 Dec 2018.
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Abstract

ABSTRAK

Tujuan penelitian ini adalah mengetahui pengaruh paparan medan magnet 0.2 mT pada ion logam Fe dan Zn dalam media pertumbuhan terhadap produksi protease Bacillus sp.  Ion logam yang digunakan yaitu Fe dan Zn dalam bentuk garam FeCl3 dan ZnCl2. Hasil penelitian menunjukkan bahwa ion logam Fe (0.01% b/v) yang terpapar medan magnet   mempunyai nilai Indeks Proteolitik 3,36 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,74 dengan lama inkubasi 18 jam. Sedang ion logam Fe (0.01% b/v) yang tidak terpapar medan magnet mempunyai nilai Indeks Proteolitik 1,79 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,12 dengan lama inkubasi 18 jam. Sedangkan pada ion logam Zn (0.005% b/v) yang terpapar medan magnet dan tidak terpapar medan magnet menyebabkan aktivitas Bacillus mati. Larutan ion logam Fe 0,01% dalam media cair menghasilkan produksi protease lebih baik (0,06 U/ml) dibandingkan dengan produksi protease tanpa dipemapar medan magnet (0,00 U/ml).

Kata kunci: Bacillus sp., paparan medan magnet, indeks proteolitik.

ABSTRACT

The purpose of this study was to determine the effect of metal ions Fe and Zn were exposed to a magnetic field of 0.2 mT to the production of proteases in Bacillus sp. The metal ions used are Fe and Zn in the form of FeCl3 and ZnCl2 salts. The results showed that the culture medium containing metal ions Fe (a 0.01% w/v) were exposed by the magnetic field has a value of proteolytic index of 3.36 with 10 hour long incubation, and proteolysis index of 2.74 with 18 hour incubation time. Culture medium containing metal ions Fe (a 0.01% w/v) which are not being exposed to the magnetic field has a value of proteolytic index of 1.79 with 10-hour long incubation, and proteolysis index of 2.12 with 18 hour incubation time. While the culture medium containing metal ions Zn (0.005% w/v) either exposed or not exposed to the magnetic field causes the activity of Bacillus sp die. The solution of 0.01% Fe metal ion in liquid medium resulted in better protease production (0.06 U/ml) than production of proteases without exposure to magnetic fields (0.00  U/ml)

Keywords: Bacillus sp., magnetic field, proteolytic index, and proteolysis index

Sitasi: Sumardi, Agustrina R., Irawan B., dan I. Selfiana. (2018). Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp. Jurnal Ilmu Lingkungan, 16(2), 173-177,doi:10.14710/jil.16.2.173-177

Keywords
Bacillus sp., magnetic field, proteolytic index, and proteolysis index

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