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Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp

Jurusan Biologi, FMIPA Universitas Lampung Jl. Sumantri Brojonegoro No. 1 Gedong Meneng Bandar Lampung, Indonesia

Received: 19 Feb 2018; Published: 15 Dec 2018.
Editor(s): H Hadiyanto

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Abstract

ABSTRAK

Tujuan penelitian ini adalah mengetahui pengaruh paparan medan magnet 0.2 mT pada ion logam Fe dan Zn dalam media pertumbuhan terhadap produksi protease Bacillus sp.  Ion logam yang digunakan yaitu Fe dan Zn dalam bentuk garam FeCl3 dan ZnCl2. Hasil penelitian menunjukkan bahwa ion logam Fe (0.01% b/v) yang terpapar medan magnet   mempunyai nilai Indeks Proteolitik 3,36 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,74 dengan lama inkubasi 18 jam. Sedang ion logam Fe (0.01% b/v) yang tidak terpapar medan magnet mempunyai nilai Indeks Proteolitik 1,79 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,12 dengan lama inkubasi 18 jam. Sedangkan pada ion logam Zn (0.005% b/v) yang terpapar medan magnet dan tidak terpapar medan magnet menyebabkan aktivitas Bacillus mati. Larutan ion logam Fe 0,01% dalam media cair menghasilkan produksi protease lebih baik (0,06 U/ml) dibandingkan dengan produksi protease tanpa dipemapar medan magnet (0,00 U/ml).

Kata kunci: Bacillus sp., paparan medan magnet, indeks proteolitik.

ABSTRACT

The purpose of this study was to determine the effect of metal ions Fe and Zn were exposed to a magnetic field of 0.2 mT to the production of proteases in Bacillus sp. The metal ions used are Fe and Zn in the form of FeCl3 and ZnCl2 salts. The results showed that the culture medium containing metal ions Fe (a 0.01% w/v) were exposed by the magnetic field has a value of proteolytic index of 3.36 with 10 hour long incubation, and proteolysis index of 2.74 with 18 hour incubation time. Culture medium containing metal ions Fe (a 0.01% w/v) which are not being exposed to the magnetic field has a value of proteolytic index of 1.79 with 10-hour long incubation, and proteolysis index of 2.12 with 18 hour incubation time. While the culture medium containing metal ions Zn (0.005% w/v) either exposed or not exposed to the magnetic field causes the activity of Bacillus sp die. The solution of 0.01% Fe metal ion in liquid medium resulted in better protease production (0.06 U/ml) than production of proteases without exposure to magnetic fields (0.00  U/ml)

Keywords: Bacillus sp., magnetic field, proteolytic index, and proteolysis index

Sitasi: Sumardi, Agustrina R., Irawan B., dan I. Selfiana. (2018). Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp. Jurnal Ilmu Lingkungan, 16(2), 173-177,doi:10.14710/jil.16.2.173-177

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Keywords: Bacillus sp., magnetic field, proteolytic index, and proteolysis index

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  1. Agustrina R, Handayani T T, dan Sumardi. 2013. Observation of the effect of static magnetic field 0.1 mt on -amylase activity in legume germination. Proseding 2nd International Conference on Engineering and Technology Development (ICETD) Faculty of Engineering and Faculty of Computer Science
  2. Bacillus Sp. Jurnal Penelitian Pertanian Terapan Vol. 16 (2): 76 - 84 http://www.jptonline.or.id
  3. Bergmeyer dan Grassl, 1983. Methods of Enzymatic Analysis 2. Verlag Chemia. Weinhein
  4. Durham, D. R., D. B. Stewart, and E. J. Stellwag. 1987. Novel alkaline and heat stable serine proteases from alkalaphilic Bacillus sp. strain GX6638. J. Bacterial., 169(6): 2762- 2768
  5. field 0.2 mt. IOP Conf. Series: Earth and Environmental Science 130 012046 doi :10.1088/1755-1315/130/1/012046
  6. He, S., Y. Feng, H. Ren, and Y. Zhang. 2011. The Impact Of Iron Oxide Magnetic Nanoparticles On The Soil Bacterial Community. School of Biological Science and Medical Engineering. 11: 1408-1417. China
  7. Hernawati W, Sumardi, Agustrina R, dan Yulianto H. 2016. Pengaruh Pemaparan Medan Magnet Pada Media Mandels Yang Dimodifikasi Terhadap Pertumbuhan Dan Aktivitas Enzim Selulase, ISSN 1410-5020 eISSN Online 2047-1781
  8. Kementerian Lingkungan Hidup. 2014. Peraturan Menteri Lingkungan Hidup No. 5 Tahun 2014 Tentang Baku Mutu Air Limbah
  9. Kobya, M., E. Sentruk, C. Aydiner and E. Demirbas. 2006. Treatment of potato chips manufacturing waste water by electroagulation. Desalination, 190: 201-211
  10. Kundu, P., A. Dabsarkar, S. Mukherjee. 2013. Treatment of Slaughter House Wastewater in a sequencing Batch Reactor, Performance evaluation and Biodegradation Kinetics. Hindawi Publishing Corporation, BioMed Research International Article ID134872, II pages
  11. Lie, Jie. 2015. Study on the effect of magnetic field treatment of newly isolated Paenibacillus sp. Botanical Studies (2015) 56:2
  12. Rumah Potong Hewan Ruminansia dan Unit Penanganan daging (meat cutting plant)
  13. Said, M.I dan J.C. Likadja. 2012. Isolasi dan Identifikasi Bakteri yang Berpotensi Sebagai Penghasil Enzim Protease Pada Industri Penyamakan Kulit PT. Adhi Satria Abadi (ASA), Yogyakarta. JITP Vol. 2 No. 2. UGM
  14. Sari. E.K.N., Susilo. B., danSumarlan.S.lH. 2012. Proses Pengawetan Sari Buah Apel (Mallussylvertris Mill) Secara Non Thermal Berbasis Teknologi Oscillating Magnetic Field (OMF). Jurnal Teknologi Pertanian Vol.13 No.2 (Agustus 2012) 78-87
  15. Soeka Y. S., dan Sulistiani. 2014. Karakterisasi Protease Bacillus Subtilis A1 Inacc B398 yang Diisolasi Dari Terasi Samarinda. Berita Biologi 13(2)
  16. Sudarti, Nurhayati, Eka Ruriani, Vonni Triana Hersa. 2014. Prevalence of Salmonella Typhimurium on Gado-Gado Seasoning by Treatment of Extremely Low Frequency (ELF) Magnetic Field.Artikel-ELF-Salmonella. Jember University
  17. Sumardi, Agustrina R, Ekowati C.N., and Pasaribu Y S. 2018. Characterization of protease from bacillus sp. on medium containing FeCl3 exposed to magnetic
  18. Sumardi, C. N. Ekowati dan D. Handayani. 2010. Isolasi Bacillus Penghasil Selulase dari Saluran Pencernaan Ayam Kampung. Jurnal Sains Mipa, April 2010, Vol. 16, No.1, Hal:62-68. Universitas Lampung
  19. Sutresna. 2006. Fisika Dasar: Lisrik, magnet, dan termofisika.penerbit ITB. Bandung
  20. Trianie, FA. 2014. Pengaruh fortifikasi besi dan zinc terhadap Total bakteri asam laktat, ph, dan Organoleptik yoghurt susu kambing sinbiotik. Fakultas Kedokteran Universitas Diponegoro Semarang
  21. Vidyasagar, S.B. Prakash and K. Sreeramulu. 2006. Optimization of culture conditions for the production of haloalkaliphilic thermostable protease from an extremely halophilic archaeon Halogeometricum sp. TSS101 M. The Society for Applied Microbiology, Letters in Applied Microbiology 43: 385–391
  22. Vijayaraghavan, Ponnuswamy dan Samuel Gnana Prakash Vincent. 2013. A simple method for the detection of protease activity on agar plates using bromocresolgreen dye. J Biochem Tech 4(3): 628-630
  23. Yusufa, Mohammad H., Masdiana C. Padaga, Dyah A., dan Octavianie. 2012. Identifikasi dan studi aktivitas protease Bacillus sp asal limbah cair rumah potong ayam tradisional sebagai kandidat penghasil biodeterjen. Universitas Brawijaya
  24. Zahidah, D. dan M. Shovitri. 2013. Isolasi, Karakterisasi dan Potensi Bakteri Aerob Sebagai Pendegradasi Limbah Organik. Jurnal sains dan seni pomits vol. 2, no.1. 12-15

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