DOI: https://doi.org/10.14710/jil.24.5.1116-1123

Perbandingan Kemampuan Dekolorisasi Naftol Merah dan Naftol Biru oleh Bacillus velezensis IBLTT_1, Bacillus cereus IBLTT_5, dan Enterococcus faecalis

Departement of Biology, Universitas Kristen Satya Wacana, Jl. Diponegoro no. 52-60, Sidorejo, Salatiga, Indonesia 50711, Indonesia

Received: 9 Jun 2026; Revised: 28 Aug 2026; Accepted: 2 Sep 2026; Available online: 29 Sep 2026; Published: 30 Sep 2026.
Editor(s): Budi Warsito

Citation Format:
Abstract

Dekolorisasi pewarna sintetis oleh bakteri merupakan salah satu pendekatan biologis yang dapat digunakan untuk mengurangi warna pada limbah yang mengandung pewarna. Penelitian ini bertujuan membandingkan kemampuan tiga strain bakteri, yaitu Bacillus velezensis IBLTT_1, B. cereus IBLTT_5, dan Enterococcus faecalis, dalam mendekolorisasi naftol merah dan naftol biru. Penelitian dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap dengan dua faktor, yaitu strain bakteri dan jenis pewarna, dengan empat ulangan biologis pada setiap kombinasi perlakuan. Dekolorisasi dievaluasi setelah inkubasi selama 48 jam berdasarkan persentase dekolorisasi dan laju dekolorisasi, sedangkan efisiensi relatif dekolorisasi digunakan sebagai parameter deskriptif. Data persentase dan laju dekolorisasi dianalisis menggunakan Two-Way ANOVA setelah pengujian asumsi dan transformasi data sesuai kebutuhan, dengan uji lanjut Bonferroni. Hasil penelitian menunjukkan bahwa E. faecalis memiliki persentase dekolorisasi tertinggi terhadap naftol merah (80,03 ± 0,18%) dan naftol biru (93,68 ± 0,81%), sedangkan B. velezensis IBLTT_1 menunjukkan persentase dekolorisasi 73,22 ± 1,12% dan 30,73 ± 1,17%, serta B. cereus IBLTT_5 sebesar 50,28 ± 1,34% dan 66,96 ± 2,24% terhadap naftol merah dan naftol biru. Strain bakteri, jenis pewarna, dan interaksi keduanya berpengaruh signifikan terhadap persentase dan laju dekolorisasi (P < 0,05). Hasil ini menunjukkan bahwa kemampuan dekolorisasi berbeda antarstrain dan bergantung pada jenis pewarna. Namun, hasil dekolorisasi tidak dapat secara langsung diinterpretasikan sebagai biodegradasi karena produk transformasi dan aktivitas enzim yang terlibat tidak dianalisis.

Fulltext View|Download Email colleagues
Keywords: bakteri; Bacillus; dekolorisasi; Enterococcus faecalis; naftol biru; naftol merah

Article Metrics:

Article Info
Section: Research Article
Language : ID
Most cited articles
PENGARUH FAKTOR-FAKTOR SOSIAL-EKONOMI TERHADAP PERILAKU PENGELOLAAN SAMPAH DOMESTIK DI NUSA TENGGARA TIMUR PERBAIKAN KUALITAS AIR LIMBAH DOMESTIK DENGAN FITOREMEDIASI MENGGUNAKAN KOMBINASI BEBERAPA GULMA AIR: STUDI KASUS KOLAM RETENSI TALANG AMAN KOTA PALEMBANG RANCANG BANGUN DAN UJI ALAT PROSES PENINGKATAN MINYAK CENGKEH PADA KLASTER MINYAK ATSIRI KABUPATEN BATANG Evaluasi Penerapan Program Adiwiyata Untuk Membentuk Perilaku Peduli Lingkungan di Kalangan Siswa (Kasus: SMA Negeri 9 Tangerang Selatan dan MA Negeri 1 Serpong) PENGELOLAAN SAMPAH RUMAH TANGGA DI KECAMATAN DAHA SELATAN More cited articles
  1. Al-Garni, S.M., Ghanem, K.M., Kabli, S.A., and Biag, A.K., (2013), Decolorization of Crystal Violet by Mono and Bacterial Culture Techniques Using Optimized Culture Conditions. Polish Journal of Environmental Studies, 22(5), 1297-1306
  2. Aprilia, T., (2022), Pewarna Batik Zat Sintetis di Rumah Batik Pandan Mengurai Kota Sungai Penuh. Gorga: Jurnal Seni Rupa, 11(1), 2301-5942
  3. Aragaw, T. A. (2024)a, Potential and Prospects of Reductases in Azo Dye Degradation: A Review. The Microbe, 4, 100162
  4. Aragaw, T.A., (2024)b, A Review of Dye Biodegradation in Textile Wastewater, Challenges Due to Wastewater Characteristics, and the Potential of Alkaliphiles. Journal of Hazardous Materials Advances, 16, 100493
  5. Benammar, H.S., Guergazi, S., Youcef, S., and Youcef, L., (2021), Removal of Congo Red and Naphthol Blue Black Dyes from Aqueous Solution by Adsorption on Activated Carbon. Characterization, Kinetic and Equilibrium in Nonlinear Models Studies. Desalination and Water Treatment, 221, 396-405
  6. Carrascal-Hernández, D.C., Orozco-Beltrán, E.J., Insuasty, D., Márquez, E., and Grande-Tovar, C.D., (2025), Systematic Evaluation of Biodegradation of Azo Dyes by Microorganisms: Efficient Species, Physicochemical Factors, and Enzymatic Systems. International Journal of Molecular Sciences, 26(16), 7973
  7. El Awady, M.E., El-Shall, F.N., Mohamed, G.E., Abd-Elaziz, A.M., Abdel-Monem, M.O., and Hassan, M.G., (2024), Exploring the Decolorization Efficiency and Biodegradation Mechanisms of Different Functional Textile Azo Dyes by Streptomyces albidoflavus 3MGH. BMC Microbiology, 24, 210
  8. Ferkous, H., Hamdaoui, O., and Merouani, S., (2015), Sonochemical Degradation of Naphthol Blue Black in Water: Effect of Operating Parameters. Ultrasonics Sonochemistry, 26, 40-47
  9. Goswami, D., Mukherjee, J., Mondal, C., and Bhunia, B., (2024), Bioremediaztion of Azo Dye: A Review on Strategies, Toxicity Assessment, Mechanisms, Bottlenecks and Prospects. Science of The Total Environment, 954, 176426
  10. Handayani, W., Meitiniarti, V.I., and Timotius, K.H., (2007), Decolorization of Acid Red 27 and Reactive Red 2 by Enterococcus faecalis Under a Batch System. Word Journal of Microbiology and Biotechnology, 23(9), 1239-1244
  11. Harsini, M., Suyanto, Y.G.Y., Rhodifasari, L., and Darmokoesomo, H., (2017), Electrochemical Degradation of Naphthol AS-BO Batik Dyes. Journal of Chemical Technology and Metallurgy, 52(6), 1116-1122
  12. Hubbe, M.A., Beck, K.R., O'Neal, W.G., and Sharma, Y.C., (2012), Cellulosic Substrates for Removal of Pollutants from Aqueous Systems: A Review. 2. Dyes, BioResources, 7(2), 2592-2687
  13. Hsueh, C.C., Chen, B.Y., and Yen, C.Y., (2009), Understanding Effects of Chemical Structure on Azo Dye Decolorization Characteristics by Aeromonas hydrophila. Journal of hazardous materials, 167(1-3), 995-1001
  14. Khan, S., and Borah, D., (2024), Microbial Cell Factories in the Degradation of Azo-Dye and Their Limiting Factors: An Insight. Cleaner Water, 2, 100034
  15. Khongthaw, B., Chauhan, P.K., Chishty, N., Kumar, D., Velmurugan, A., Singh, A., Bhtoya, R., Devi, N., Nene, A., Sadeghzade, S., and Ighalo, J.O., (2024), Aqueous Phase Textile Dye Degradation by Microbes and Nanoparticles: A Review. Advances in Materials Science and Engineering, 2024, 8845873
  16. Kumaravel, R., and Shanmugam, V.K., (2024), Biomimetic and Ecological Perspective Towards Decolorization of Industrial Important Azo Dyes Using Bacterial Cultures – A Review. Sustainable Chemistry for the Environment, 7, 100130
  17. Meitiniarti, V.I., Napitupulu, M.M., and Timotius, K.H., (2004), Pertumbuhan Curah Enterococcus faecalis ID6017 dan Kemampuan Dekolorisasi Reactive Red-2 pada Medium yang Mengandung Gliserol. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 9(1), 19-24
  18. Meitiniarti, V.I, Timotius, K.H., Soetarto, E.S., and Sugiarto, E., (2012), Decolorization of Orange II by Mixed Culture of Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016. Microbiology Indonesia, 6(3),117-123
  19. Moharrery, L., Ardestani, N.S., and Otadi, M., (2025), Bio Decolorization of the Oil Soluble Azo Dye Toluidine Red by Halomonas strain A3. Scientific Reports, 15(1), 33249
  20. OECD, (2017), Use of Textile Dyes. OECD Series on Emission Scenario Documents, No. 36. OECD Publishing, Paris
  21. Permatasari, I., Nugroho, R.A., and Meitiniarti, V.I., (2018), Dekolorisasi Pewarna Tekstil Sumifix Blue dan Reactive Red 2 oleh Mikroba yang Diisolasi dari Limbah Industri Tekstil. Jurnal Bioteknologi dan Biosains Indonesia, 5(1), 2548-611
  22. Prakoso, T.H., Firda W., Annisa M.S., Yora F., and Happy. W., (2022), Dekolorisasi Pewarna Tekstil Menggunakan Teknik Batch dan Rotary Biological Contraktor Dengan Tiga Jenis Agen Hayati. Jurnal Teknologi Industri Pertanian, 32(3), 295-304
  23. Tripathi, M., Singh, S., Pathak, S., Kasaudhan, J., Mishra, A., Bala, S., Garg, D., Singh, R., Singh, P., Singh, P.K., Shukla, A K., and Pathak, N., (2023), Recent Strategies for the Remediation of Textile Dyes from Wastewater: A Systematic Review. Toxics, 11(11), 940
  24. Veluchamy, A., Jeyabalan, J., Singh, A., Narayanasamy, S., and Verma, A., (2024), A Review on Recent Insights of Azoreductases Mediated Dye Degradation: A Sustainable Approach for Bioremediation of Industrial Wastewater. Journal of Water Process Engineering, 68, 106403
  25. Wang, R., Li, H., Liu, Y., Chen, J., Peng, F., Jiang, Z., Liu, J., and Song, H., (2022), Efficient Removal of Azo Dyes by Enterococcus faecalis R1107 and Its Application in Simulated Textile Effluent Treatment. Ecotoxicology and Environmental Safety, 238, 113577

Last update:

No citation recorded.

Last update: 2026-09-30 17:12:46

No citation recorded.