Status antioksidan dan oksidatif laki-laki yang mengalami kegemukan dengan pemberian minuman rosela ungu

*Sriyanti Sriyanti  -  Departemen Gizi Masyarakat, Fakultas Ekologi Manusia, Institut Pertanian Bogor, Indonesia
Evy Damayanthi  -  Departemen Gizi Masyarakat, Fakultas Ekologi Manusia, Institut Pertanian Bogor, Indonesia
Faisal Anwar  -  Departemen Gizi Masyarakat, Fakultas Ekologi Manusia, Institut Pertanian Bogor, Indonesia
Received: 17 Jan 2019; Published: 14 Jun 2019.
DOI: View
Surat Pernyataan keaslian dan Persetujuan Penulis
Type Other
  Download (378KB)    Indexing metadata
Open Access
Citation Format:

Background : Rosela (Hibiscus sabdariffa L.) is an anthocyanin-rich plant that has good antioxidant capacity. However, the utilization was limited to red roselle, while previous studies showed that anthocyanin content was found to be higher in the thick roselle.therefore, this is an opportunity to develop purple roselle products as a preventive effort for degenerative diseases in men with fatness. Subjects in this study were male by considering the effect of estrogen on women which could play a role in suppressing oxidative stress.

Objectives : This study aimed to analyze antioxidant (SOD/Superoxide dismutase) and oxidative status (MDA/malondialdehyde) level in obese adult men after administration of purple rosela drinks

Methods : This study was quasi-experimental with pre-post test. Eighteen obese subjects in the security unit were divided into two groups, namely the intervention group and control group. Intervention group (n = 9) was given purple rosela drinkin the amount of 480 mL/day, while the control group (n = 9) was given an equivalent amount of purple rosela drink without rosela. The subjects were daily administered for 6 weeks. Characteristic, anthropometric, food consumption and blood sample data were collected at the beginning and end of the intervention. Data analysis was performed by descriptively, paired t-test, independent t-test, ANCOVA.

Results : The analysis showed that purple rosela drink tend to increase SOD levels (0.45 ± 0.14; 1.35 ± 0.71; p-value = 0.008) but this value was not significantly different from the control group (p-value = 0.128). MDA levels did not significantly different between the intervention and control groups (0.025 ± 0.083;-0.125 ± 0.60; p-value = 0.473).

Conclusion : These results showed that administering 480 ml of purple rosela drink did not improve antioxidant and oxidative status in obese adult men.

Note: This article has supplementary file(s).

Keywords: anthocyanin; antioxidant; obesity; purple roselle; stres oksidatif

Article Metrics:

  1. WHO. Obesity and Overweight [internet]. Media Center World Health Organization. 2017 (diakses pada 1 Agustus 2017) di alamat
  2. Park KY, Park HK, Hwang HS. Relationship between abdominal obesity and alcohol drinking pattern in normal-weight, middle-aged adults: the korea national health and nutritional examination survey 2008-2013. Publ H Nutr. 2017. 20(12): 2192-2200. doi: 10.1017/S1368980017001045
  3. Selvant, Ramadurg, Dorle, Ghattargi, Banada. A Cross-Sectional Study on Pattern of Alcohol Consumption and Body Mass Index Among Health Institution Students in Bagalkot. J Clin Diagn Res. 2015. 9(12): 6-9
  4. Canoy D, Wareham N, Luben R, Welch A, Bingham S, Day N, Khaw KT. Cigarette Smoking and Fat Distribution in 21. 828 British Men and Women: A Populationbased Study. Obes Res. 2005. 13(2005)
  5. Drapeau V, Despres JP, Bouchard C, Allard L, Fournier G, Leblanc C, Tremblay A. Modification in food-group consumption are related to long-term body-weight changes. AJCN. 2004. 80(2004):29-37. doi: 10.1093/ajcn/80.1.29
  6. Jakicic JM, Greg E, Knowler W, Kelley DE, Lang W, Miller GD, Pi-Sunyier FX, Regensteiner JG, Rejeski WJ, Ribisl P, et al. Activitiy patterns of obese adults with type 2 diabetes in the Look AHEAD study. Med Sci Sports Exerc. 2010. 42(11):1995-2005. doi: 10.1249/MSS.0b013e3181e054f0
  7. Vincent HK, Innes KE, Vincent KR. Oxidative stress and potential interventions to reduce oxidative stress in overweight and obesity. Diabetes, Obes, Met. 2007. 9(2007):813-839. doi: 10.1111/j.1463-1326.2007.00692.x
  8. Amirkhizi F, Siassi F, Djalali M, Shahraki SH. Impaired enzymatic antioxidant defense in erythrocytes of women with general and abdominal obesity. Obes Res Clin Prac. 2014. 8(2014):e26-e34. doi: 10.1016/j.orcp.2012.07.004
  9. Basu A, Betts NM, Ortiz J, Simmons B, Wu M, Lyons TJ. Low-energy cranberry juice decrease lipid oxidation and increases plasma antioxidant cpacity in women with metabolic syndrome. Nutr Res. 2011. 31(2011):190-196. doi: 10.1016/j.nutres.2011.02.003
  10. Jansakova K, Babickova J, Havrientova M, Hodosy J, Kraic J, Celec P, Tothova L. The effects of anthocyanin rich wheat diet on the oxidative status and behavior of rats. Croat Med J. 2016. (57):119-129. doi: 10.1016/j.jep.2003.09.008
  11. Qin Y, Xia M, Ma J, Ho Y, Liu J, Mou H, Cao L, Ling W. Anthocyanin supplementation improves serum LDL- and HDL-cholesterol concentrations associated with the inhibition of cholesteryl ester transfer protein in dyslipidemic subjects. Am J Clin Nutr. 2009. (90):485-492. doi: 10.3945/ajcn.2009.27814
  12. Suarez JMA, Giampieri F, Tulipani S, Casoli T, Stefano GD, Ana M, Paramas G, Buelga CS, Busco F, Quiles JL, et al. One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress markers and platelet activation in humans. J Nutr Biochem. 2014. (25):289-294. doi: 10.1016/j.jnutbio.2013.11.002
  13. Basu A, Du M, Leyva MJ, Sanchez K, Betts NM, Wu M, Aston CE, Lyons TJ. Blueberries decrease cardiovasculer risk factors in obese men and women with metabolic syndrom. J Nutr. 2010. 140(2010):1582-1587. doi: 10.3945/jn.110.124701
  14. Zhu Y, Ling W, Guo H, Song F, Ye Q, Zou T, Li D, Zhang Y, Li G, Xiao Y et al. Anti-inflammatory effect of purified dietary anthocyanin in adults with hypercholesterolemia: A randomized controlled trial. Nutr, Metabolisme and Cardiovascular Disease. 2013. 23:843-849. doi: 10.1016/j.numecd.2012.06.005
  15. Vendrame S, Daugherty A, Kristo AS, Riso P, Zacas DK. Wild blueberry (Vaccinium angustifolium) consumption improves inflammatory status in the obese Zucker rat model of the metabolic syndrome. J Nutr Biochem. 2013. (24):1508-1512. doi: 10.1016/j.jnutbio.2012.12.010
  16. Seymour EM, Tanone II, Daniel E, Llanes U, Lewis Sk, Kirakosyan A, Kondoleon MG, Peter B, Kaufman, Bolling SF. J Med Food. 2011. 14(12):1511-1518. doi: 10.1089/jmf.2010.0292
  17. Wu T, Tang Q, Gao Z, Yu Z, Song H, Zheng X, Chen W. Blueberry and Mulberry Juice Prevent Obesity Development in C57BL/6 Mice. Plos One. 2013. 8(10):e77585
  18. Suzery M, Lestari S, Cahyono B. Penentuan total antosianin dari kelopak bunga rosela (Hibiscus sabdariffa L) dengan metode maserasi dan sokshletasi. J Sains Math. 2010. 18(1):1-6
  19. Djaeni M, Ariani N, Hidayat R, Utari FD. Ekstrak antosianin dari kelopak bunga rosela (Hibiscus sabdariffa L.) berbantu ultrasonik:tinjauan aktivitas antioksidan. J Apl Tekn Pang. 2017. 6(3):148-151. doi: 10.17728/jatp.236
  20. Ezzat SM, Salama MM, el-Din SHS, Saleh S, Lakkany NME, Hammam OA, Salem MB, Botros SS. Metabolyc profile and hepatoprotective activityof the anthocyanin-rich etract of Hibiscus sabdariffa calyces. Pharm Biol. 2016. 1(2016):1-10. doi: 10.1080/13880209.2016.1214739
  21. Ilyas EII, kartinah NT, Andriani T, Goernarjo RA, Kahanjak DN, Freisleben HJ. Effects of Hibiscus sabdariffa Linn. on malondialdehyde and glutathione peroxide in an overtraining rat model characterized by growth hormone, insulin-like growth factor-1, and insulin-like growth factor binding potein-3. Biomed Pharm J. 2017. 10(1):19-27. doi: 10.13005/bpj/1076
  22. Hadi A, Pourmasoumi M, kafeshani M, Karimiah J, Maracy MR, Entezari MH. The effect of green tea and sour tea (Hibiscus sabdariffa L.) supplementation on oxidative stress and muscle damage in athletes. J Diet Supp. 2016. 0(0):1-12. doi: 10.1080/19390211.2016.1237400
  23. Christian KR, Jackson JC. Changes in total phenolic and monomeric anthocyanin composition and antioxidant activity of three varieties of sorrel (Hibiscus sabdariffa) during maturity. J Food Composit and Anal. 2009. 22:663-667. doi: 10.1016/j.jfca.2009.05.007
  24. Asgary S, Soltani R, Zolghadr M, Keshvari M, Sarrafzadegan N. Evaluation of the effects of roselle (Hibiscus sabdariffa L.) on oxidative stress and serum levels of lipids, insulin, and hs-CRP in adults patients with metabolic syndrome: a double blind placebo-controlled clinical trial. J Complement Integr Med. 2016. (2016):1-6. doi: 10.1515/jcim-2015-0030
  25. Serra LM, Bautista IC. Etiology of obesity: two “key issues” and other emerging factors. Nutr Hosp. . 2013. 28(5):32-43
  26. Zhao D, Li Y, Zheng L, Yu K. Brief Communication: Body Mass Index, Body Adiposity Index, and Percent Body Fat in Asians. American J Phys Anthro. 2013. (00):00-00.doi: 10.1002/ajpa.22341
  27. Thibault H, Contrand B, Saubusse E, Baine M, Tison SM. Risk factor for overweight and obesity in French adolescent: Physical activity, sedentary behavior and parental characteristics. Nut. 2010. 26(2010):192-200. doi: 10.1016/j.nut.2009.03.015
  28. Jakicic JM, Kelliann K, Davis. Obesity and physical activity. Psychiatr Clin N Am. 2011. 34(2011):829-840. doi: 10.1016/j.psc.2011.08.009
  29. Dam RM, Seidell J. Carbohydrate intake and obesity. European J Clin Nutr. 2007. 61(1):575-599
  30. Azzini E, Giacometti J, Russo GL. 2017. Antiobesity effects of anthocyanins in preclinical and clinical studies. Hindawi. 2017:1-12.doi: 10.1155/2017/2740364
  31. Aguilar F, Zamilpa A, Garcia D, Almanza J, Romero E, Campos E, Carillo L, Ramos R. 2007. Effect of Hibiscus sabdariffa on obeity in MSG mice. J Ethnopharmacology. 114:66-71.doi: 10.1016/j.jep.2007.07.020
  32. DeFuria J, Bennett G, Strissel et al. Dietary blueberry attenuates whole-body insulin resistance in high fat-fed mice by reducing adipocyte death and its inflammatory sequelae. J Nutr. 2009. 139:1510-1516
  33. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M, Shimomura I. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Inves. 2004. (114):1752-1761. doi: 10.1172/JCI200421625
  34. Esa NM, Hern FS, Ismail A, Yee CL. Antioxidant activity in different parts of roselle (Hibiscus sabdariffa L.) extracts and potential exploitation of the seeds. Food Chem. 2010. (122):1055-1060. doi: 10.1016/j.foodchem.2010.03.074
  35. Wong PK, Yusof S, Ghazali HM, Man YBC. Physico-chemical characteristics of roselle (Hibiscus sabdarriffa L.). Nutr Food Scien. 2002. 32(2): 68-73. doi: 10.1108/00346650210416994
  36. Juniarka IGA, Lukitaningsih E, Noegrohati S. Analisis aktivitas antioksidan dan kandungan antosianin total ekstrak dan liposom kelopak bunga rosela (Hibiscus Sabdariffa L.). Majalah Obat Tradisi. 2011. 16(3):115-123
  37. Tanaka Y, Shiozawa S, Morimoto I, Fujita T. Zinc inhibit pokeweed mitogen-induced development of immunoglobulin-secreting cells through augmentation of CD4 and CD8 cells. Int J Immunopharmachol. 1989. 11(6):673-679777

Last update: 2021-03-01 08:32:12

No citation recorded.

Last update: 2021-03-01 08:32:13

No citation recorded.