DOI: https://doi.org/10.14710/jgi.11.2.77-84

STUDY OF VITAMIN D3-FORTIFIED GOAT KEFIR ON PLASMA FIBRINOGEN LEVELS OF DIABETIC RATTUS NORVEGICUS RATS

1Department of Nutrition, Faculty of Medicine, Universitas Diponegoro, Indonesia

2Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Diponegoro, Indonesia

3Center of Nutrition Research, Universitas Diponegoro, Indonesia

Received: 11 Apr 2022; Published: 24 Jun 2023.

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Abstract

ABSTRACT

Background: Diabetes mellitus is often associated with the occurrence of complications. Haemostatic factors, especially hyperfibrinogenaemia, is a common cause of the complication. Goat kefir and vitamin D3 may act as an antioxidant and anti-inflammation agent which can repair pancreatic beta cells.

Objectives: This study aimed to analyse the effect of vitamin D3-fortified goat milk and plasma fibrinogen levels in diabetic rats.

Materials and Methods: This study was an experimental study with pre-post only group design. The samples were 21 male rats divided into four groups; negative control (K-), positive control (K +), treated with unfortified goat kefir (P1), and treated with vitamin D3-fortified goat kefir (P2). The 35-day intervention was conducted, the goat kefir dose was 2 ml/200 g BW/day and the vitamin D dose 600 IU. Fasting blood glucose and plasma fibrinogen were assessed pre- and post-intervention. Blood glucose level was evaluated by GOD-PAP method, while plasma fibrinogen was assessed by Enzyme-Linked Immunosorbent Assay (ELISA) method. The data were analysed with paired t-test and One-Way ANOVA.

Results: There were not significant difference levels of fibrinogen between groups. The intervention groups both showed an insignificant decrease of plasma fibrinogen. The plasma fibrinogen of group treated with vitamin D3-fortified goat kefir went down to 13.47 mg/dl from 16.49 mg/dl (p = 0.49). Meanwhile, the group treated with unfortified goat kefir showed a decrease from 26.81 mg/dl to 24.94 mg/dl (p=0.83). On the other hand, there was a significant decrease in fasting blood glucose in the group treated with vitamin D3-fortified goat kefir from 181.75 mg/dl to 116.25 mg/dl (p=0.03).

Conclusion Our results demonstrate that administration of vitamin D3-fortified goat kefir can decrease fasting blood glucose but not in plasma fibrinogen. 

Keywords : Diabetes Mellitus; Fastin blood glucose; Fibrinogen; Goat kefir; Vitamin D3 Fortification

 

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Keywords: Diabetes Mellitus; Fastin blood glucose; Fibrinogen; Goat kefir; Vitamin D3 Fortification

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