skip to main content

NOVEL SNP OF CALPAIN-1 (CAPN1) GENE AND ITS ASSOCIATION WITH CARCASS AND MEAT CHARACTERISTICS TRAITS IN BALI CATTLE

*N. Pratiwi  -  Graduate School of Animal Science, Bogor Agricultural University, Indonesia
M. Maskur  -  Faculty of Animal Science, Mataram University, Indonesia
R. Priyanto  -  Faculty of Animal Science, Bogor Agricultural University, Indonesia
J. Jakaria  -  Faculty of Animal Science, Bogor Agricultural University, Indonesia

Citation Format:
Abstract

Calpain-1 gene (CAPN1) produces an calpain enzyme controlling structure of meat protein and tenderness. The aims of this study were to identify Single Nucleotide Polymorphism (SNP) in exon 5 and 6 of CAPN1 gene and its associate with carcass and meat characteristic traits in bali cattle. A total of 48 bali cattles from BPTU-HMT Bali Cattle, Bali Province were used in the research. SNP in exon 5 and 6 of CAPN1 gene were identify with direct sequencing using MEGA 5 program. Analysis of polymorphism was conducted by PopGen 1.32 software to identify frequencies of genotype, allele and Hardy-Weinberg equilibrium. The association of CAPN1 gene genotype with carcass and meat characteristic traits was analyzed using Generalized Linear Model (GLM) procedure of SAS. Sequencing analysis at exon 5-6 of CAPN1 gene in Bali cattle resulted in eight polymorphic SNPs. They are c.3669T>C, c.3854G>A, c.3881T>C, c.3899C>T, c.3908C>G, c.4002C>A, c.4021G>T and c.4037A>C. The SNPs c.3669T>C, c.3854G>A and c.3899C>T were significantly (P<0.05) associated with rump thickness (RT), rump fat thickness (RFT) and marbling score (MS), while SNP c.4037A>C was not significantly associated with carcass and meat characteristic traits. The SNPs were significantly associated with carcass and meat characteristic traits namely c.3669T>C, c.3854G>A and c.3899C>T. Those SNPs may be used as candidate marker for Marker Assisted Selection (MAS) in bali cattle.

 

Fulltext View|Download
Keywords: Bali cattle; CAPN1 gene; SNP

Article Metrics:

Last update:

  1. Asociación de polimorfismos en los genes CAPN y CAST con propiedades fisicoquímicas de la carne bovina: una revisión

    José Américo Saucedo Uriarte, Ilse Silvia Cayo Colca, Clavel Diaz Quevedo, Rainer Marco López Lapa. CES Medicina Veterinaria y Zootecnia, 16 (1), 2021. doi: 10.21615/cesmvz.16.1.1
  2. Single Loci and Haplotypes in CAPN1 and CAST Genes are Associated with Growth, Biometrics, and in Vivo Carcass Traits in Santa Inês Sheep

    Alessandro Lima Machado, Ariana Nascimento Meira, Evandro Neves Muniz, Hymerson Costa Azevedo, Luiz Lehmann Coutinho, Gerson Barreto Mourão, Victor Breno Pedrosa, Luís Fernando Batista Pinto. Annals of Animal Science, 20 (2), 2020. doi: 10.2478/aoas-2020-0007
  3. New SNPs in the EDG1 gene’s 5′UTR and their associations to Bali cattle meat quality traits

    Sutikno Sutikno, Rudy Priyanto, Cece Sumantri, Saiful Anwar, Isyana Khaerunnisa, Ahmad Furqon, Jakaria Jakaria. PROCEEDINGS OF THE 9TH INTERNATIONAL SYMPOSIUM ON INNOVATIVE BIOPRODUCTION INDONESIA ON BIOTECHNOLOGY AND BIOENGINEERING 2022: Strengthening Bioeconomy through Applied Biotechnology, Bioengineering, and Biodiversity, 2972 , 2023. doi: 10.1063/5.0182895
  4. Association of Calpain gene with carcass percentage in Sumba Ongole cattle

    P. P. Agung, S. Said, E. M. Kaiin, M. Gunawan, N. D. Yanthi, N. Herlina, T. Maulana, F. Saputra. PROCEEDINGS OF THE 4TH INTERNATIONAL CONFERENCE OF ANIMAL SCIENCE AND TECHNOLOGY (ICAST 2021), 2628 , 2023. doi: 10.1063/5.0143987

Last update: 2024-03-04 21:20:23

  1. Single Loci and Haplotypes in CAPN1 and CAST Genes are Associated with Growth, Biometrics, and in Vivo Carcass Traits in Santa Inês Sheep

    Alessandro Lima Machado, Ariana Nascimento Meira, Evandro Neves Muniz, Hymerson Costa Azevedo, Luiz Lehmann Coutinho, Gerson Barreto Mourão, Victor Breno Pedrosa, Luís Fernando Batista Pinto. Annals of Animal Science, 20 (2), 2020. doi: 10.2478/aoas-2020-0007
  2. Polymorphism of ADIPOQ and EDG1 genes in Indonesian beef cattle

    Sutikno S.. Journal of the Indonesian Tropical Animal Agriculture, 43 (4), 2018. doi: 10.14710/jitaa.43.4.323-332
  3. The favorable alleles of AKIRIN2:C.*188G>A, EDG1:C.-312A>G and TTN:G.231054C>T as candidate markers for high-marbling are very low in Bali cattle

    Anwar S.. Biodiversitas, 20 (4), 2019. doi: 10.13057/biodiv/d200405