Cholesterol Assimilation of Saccharomyces cerevisiae B-18 isolated from gastrointestinal tract of Javanese duck

*L. Istiqomah  -  Research Center for Natural Product Technology (BPTBA), Indonesian Institute of Sciences (LIPI), Indonesia
M. Anwar  -  Research Center for Natural Product Technology (BPTBA), Indonesian Institute of Sciences (LIPI), Indonesia
A.S. Anggraeni  -  Research Center for Natural Product Technology (BPTBA), Indonesian Institute of Sciences (LIPI), Indonesia
E. Damayanti  -  Research Center for Natural Product Technology (BPTBA), Indonesian Institute of Sciences (LIPI), Indonesia
Received: 28 Sep 2017; Published: 24 May 2018.
Open Access
Citation Format:
Abstract

This study had a purpose of obtaining potential indigenous yeasts for assimilating cholesterol and assessed the in vitro activity of Bile Salt Hydrolase (BSH) using Chloramphenicol Yeast Glucose (CYG) media supplemented CaCl2 and Taurodeoxycholic Acid (TDCA). Yeasts were collected from the gastrointestinal tract of Indonesian native chicken (Gallus javanicus), Javanese duck (Anas javanicus), and Muscovy duck (Anas moschata). The BSH assay was performed to determine secretion of BSH from yeast strain to conjugate bile salts into cholic acid-free by measuring precipitation zone in a specific medium. The quantitative measurement to assimilate cholesterol in yeast using CYG broth contained soluble cholesterol (500 ppm) and incubated at 30°C for 72 hours. Microplate reader used to analyze cholesterol content in the supernatant. Data were analyzed using Analysis of Variance (ANOVA) with one way completely randomized. Precipitation zone found among isolates did not significantly different (P>0.05). Isolate B-18 from Javanese duck performed the highest percentage of assimilating cholesterol with the value of 51.83% and identified as S. cerevisiae. This isolate was closely related to S. cerevisiae mt 21s (accession number X00149.1)based on phylogenetic tree analysis. It could be concluded that S. cerevisiae B-18was potential for assimilating cholesterol in vitro.


Keywords: yeast; bile salt hydrolase (BSH); assimilation; cholesterol; Javanese duck

Article Metrics:

Article Info
Section: Articles
Language: EN
In Vol 43, No 2 (2018): June
Statistics: 755 575
Others articles
Breeding value of sires based on offspring weaning weight as a recommendation for selecting Kebumen Ongole Grade cattle FEASIBILITY STUDY OF ESTABLISHING AN ARTIFICIAL INSEMINATION (AI) CENTER FOR CARABAOS IN SAN ILDEFONSO, BULACAN, PHILIPPINES ADOPTION OF BIOSECURITY MEASURES BY LAYER SMALLHOLDERS THE EFFECTS OF HERBS ON MILK YIELD AND MILK QUALITY OF MASTITIS DAIRY COW CHEMICAL AND MICROBIOLOGICAL CHARACTERISTICS OF GOAT MILK KEFIR DURING STORAGE UNDER DIFFERENT TEMPERATURES ANTIMICROBIAL ACTIVITY OF BACTERIOCIN FROM INDIGENOUS Lactobacillus plantarum 2C12 AND ITS APPLICATION ON BEEF MEATBALL AS BIOPRESERVATIVE

Last update: 2021-02-28 02:29:52

  1. A simple method for analysis of Saccharomyces cerevisiae morphology by applying a high vacuum mode of the scanning electron microscopy and without chemical fixatives

    IOP Conference Series: Earth and Environmental Science, 127 , 2020. doi: 10.1088/1755-1315/462/1/012048

  2. Effect of Probiotic Saccharomyces spp. on Duck Egg Quality Characteristics and Mineral and Cholesterol Concentrations in Eggshells and Yolks

    I Gusti Nyoman Gde Bidura, Ni Wayan Siti, Desak Putu Mas Ari Candrawati, Eny Puspani, Ida Bagus Gaga Partama. Pakistan Journal of Nutrition, 18 (11), 2019. doi: 10.3923/pjn.2019.1075.1083

  3. Synergistic effect betweenLactobacillus plantarumAKK30 andSaccharomyces cerevisiaeB18 and the probiotic properties of microencapsulated cultures

    L Istiqomah, E Damayanti, A E Suryani, M F Karimy, D Maknuna, Lutfiani. IOP Conference Series: Earth and Environmental Science, 127 , 2019. doi: 10.1088/1755-1315/251/1/012062

  4. Saccharomyces cerevisiae B18 as antifungal and aflatoxin binder in vitro

    Lusty Istiqomah, Ema Damayanti, Doni Arisnandhy, Francis Maria Constance Sigit Setyabudi, Muslih Anwar. AIP Conference Proceedings, 127 , 2019. doi: 10.1063/1.5098414

Last update: 2021-02-28 02:29:53

  1. Cholesterol-lowering activity of lactic acid bacteria and yeast when used as probiotics in laying quail (Coturnix coturnix Japonica)

    Istiqomah L.. Journal of the Indonesian Tropical Animal Agriculture, 45 (4), 2020. doi: 10.14710/jitaa.45.4.305-319

  2. A simple method for analysis of Saccharomyces cerevisiae morphology by applying a high vacuum mode of the scanning electron microscopy and without chemical fixatives

    IOP Conference Series: Earth and Environmental Science, 127 , 2020. doi: 10.1088/1755-1315/462/1/012048

  3. Saccharomyces cerevisiae B18 as antifungal and aflatoxin binder in vitro

    Lusty Istiqomah, Ema Damayanti, Doni Arisnandhy, Francis Maria Constance Sigit Setyabudi, Muslih Anwar. AIP Conference Proceedings, 127 , 2019. doi: 10.1063/1.5098414

  4. The assays of bacteria-yeast consortia as probiotics candidates and their influences on nutrients utilization of Quails Diet

    Sofyan A.. Tropical Animal Science Journal, 42 (3), 2019. doi: 10.5398/tasj.2019.42.3.196

  5. Synergistic effect between Lactobacillus plantarum AKK30 and Saccharomyces cerevisiae B18 and the probiotic properties of microencapsulated cultures

    Istiqomah L.. IOP Conference Series: Earth and Environmental Science, 127 (1), 2019. doi: 10.1088/1755-1315/251/1/012062