The potential of Bacillus strains isolated from the rumen content of dairy cows as natural antibacterial and antioxidant agents for broilers

*S. Sugiharto  -  (SCOPUS ID = 55829964000, h-index = 4), Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
T. Yudiarti  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
I. Isroli  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
E. Widiastuti  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
H.I. Wahyuni  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
E. Suprijatna  -  Faculty of Animal and Agricultural Sciences, Diponegoro University, Indonesia
Received: 14 Dec 2017; Published: 24 May 2018.
Open Access
Citation Format:
Abstract

The study was conducted to investigate the in vitro antibacterial activity and antioxidant-enhancing effect of Bacillus strains isolated from the rumen content of dairy cows. The antibacterial activity of Bacillus strains was assessed against Escherichia colibased on the agar-well diffusion method. To assess the antioxidant-enhancing effect, the Bacillusstrains was mixed (prepared as multi-strains) and used to ferment herbal medicine waste (HMW). The effect was compared with that of commercial probiotic Bacillus subtilis and non fermented HWM. Results showed that each Bacillus strain was able to inhibit the growth of E. coli. Fermentation with mixed Bacillus strains tended (P=0.07) to enhance the 2,2-diphenylpicrylhydrazyl (DPPH) radicals scavenging activity and total phenolics content of HMW. In contrast, fermentation with commercial Bacillus subtilis tended (P=0.07) to decrease DPPH radical scavenging activity and total phenol of HMW. Fermentation with both Bacillus preparations increased (P<0.05) the contents of crude protein and ash in HMW, while crude fat increased  (P<0.05) in HMW fermented with Bacillus subtilis. Fermentation either with Bacillus subtilis or mixed Bacillus strains increased (P<0.05) the fiber content of HMW. In conclusion, Bacillus strains isolated from the rumen content of dairy cows showed antibacterial activity and antioxidant-enhancing effect, therefore, they are potential as feed additive to substitute synthetic antibiotics and antioxidants for broilers raised under tropical conditions.

Keywords: Antibacterial; antioxidant; Bacillus; fermentation; herbal medicine waste

Article Metrics:

Last update: 2021-04-20 21:17:49

  1. A combination of Dayak onion (Eleutherine palmifolia) extract and Lactobacillus acidophilus on antioxidant capacity and intestinal bacteria in broiler chickens

    Iis Yuanita, Dwi Sunarti, Hanny Indrat Wahyuni, Nyoman Suthama. IOP Conference Series: Earth and Environmental Science, 127 , 2020. doi: 10.1088/1755-1315/518/1/012014

Last update: 2021-04-20 21:17:49

  1. Recent advances in the incorporation of leaf meals in broiler diets

    Sugiharto S.. Livestock Research for Rural Development, 31 (7), 2019.
  2. Effect of two-step fermentation by Chrysonilia crassa and Bacillus subtilis on nutritional values and antioxidative properties of agro-industrial by-products as poultry feed ingredients

    Sugiharto S.. Journal of Advanced Veterinary and Animal Research, 5 (4), 2018. doi: 10.5455/javar.2018.e301
  3. Inclusion effect of ginger and turmeric mixture combined with Lactobacillus spp. Isolated from rumen fluid of cattle on health status and growth of broiler

    Risdianto D.. Journal of the Indonesian Tropical Animal Agriculture, 44 (4), 2019. doi: 10.14710/jitaa.44.4.423-433
  4. Simple fermentation using shrimp paste on the nutritional andfunctional properties of rice bran as a broiler chicken feed ingredient

    Sugiharto S.. Livestock Research for Rural Development, 32 (12), 2020.
  5. Intestinal microbial ecology and serum biochemistry of avian pathogenic escherichia coli infected broilers fed multistrain probiotic preparation

    Sugiharto S.. Livestock Research for Rural Development, 30 (8), 2018.
  6. Feeding Dayak onion (Eleutherine palmifolia) extract and Lactobacillus acidophilus mixture on blood biochemicals, meat quality characteristics and growth performance in broiler chickens

    Yuanita I.. Livestock Research for Rural Development, 31 (9), 2019.
  7. The effect of fermentation time and Trichoderma levels on digestibility and chemical components of Black Soldier fly (Hermetia illucens) larvae

    Mulyono M.. Livestock Research for Rural Development, 31 (10), 2019.