DETECTION OF PORK CONTAMINATION IN FRESH AND COOKED BEEF USING GENETIC MARKER MITOCHONDRIAL-DNA CYTOCHROME B BY DUPLEX-PCR

A. Ni'mah  -  Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia
Y. Kartikasari  -  Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia
A. D. Pratama  -  Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia
L. R. Kartikasari  -  Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia
B. S. Hertanto  -  Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia
*M. Cahyadi  -  Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia
Received: 30 Nov 2015; Published: 1 Mar 2016.
Open Access
Citation Format:
Abstract
By mixing with pork, beef adulteration is frequently found in the traditional  market that very disturbing Moeslem community in Indonesia. This study was conducted to detect pork contamination in fresh and cooked beef using genetic marker mitochondrial DNA cytochrome b (mt-DNA Cyt b) by duplex-PCR. A total of twelve samples was used in this study consisting six fresh meat samples and six cooked meat samples, respectively. Those beef and pork were bought from animal slaughterhouse and a supermarket in Surakarta. Cooked samples were prepared by boiling the meats in hot water at 100oC for 30 minutes. We designed pork contamination in beef in the level of 0, 1, 5, 10, 25%, respectively. The DNA genome was extracted and polymerase chain reaction (PCR) was performed using species specific primer to isolate mt-DNA Cyt b gene from the samples. The results showed that the DNA genome was successfully extracted from pork, beef, and contaminated meat samples. In addition, visualization of duplex-PCR on 1.5% agarose gel was able to detect pork contamination in both fresh and cooked beef up to very small proportion (1%). The existence of pork in beef was indicated with the presence of specific 398 bp DNA band. It can be concluded, duplex-PCR of mt-DNA Cyt b gene was very sensitive in detection of pork contamination in fresh and cooked beef.
Keywords: beef; contamination; cytochrome b; duplex-PCR; pork

Article Metrics:

Last update: 2021-02-26 05:08:26

No citation recorded.

Last update: 2021-02-26 05:08:26

  1. Detection of chicken contamination in beef meatball using duplex-PCR Cyt b gene

    Sari E.. IOP Conference Series: Materials Science and Engineering, 127 (1), 2017. doi: 10.1088/1757-899X/193/1/012010
  2. Current analytical methods for porcine identification in meat and meat products

    Zia Q.. Food Chemistry, 127 , 2020. doi: 10.1016/j.foodchem.2020.126664
  3. Identification of pork contamination in meatball using genetic marker mitochondrial DNA cytochrome b gene by duplex-PCR

    Novianty E.. IOP Conference Series: Materials Science and Engineering, 127 (1), 2017. doi: 10.1088/1757-899X/193/1/012002
  4. Color and texture analyses of meatballs made from beef, pork, rat, dog meats, and their mixtures

    Tathma F.. IOP Conference Series: Materials Science and Engineering, 127 (1), 2019. doi: 10.1088/1757-899X/633/1/012029
  5. The potential of food terrorism towards halal ecosystem

    Abd Razak M.A.. Food Research, 4 , 2020. doi: 10.26656/fr.2017.4(S1).S19
  6. Development of mitochondrial 12S rRNA gene for identification of dog and rat in beef using multiplex PCR

    Cahyadi M.. Journal of the Indonesian Tropical Animal Agriculture, 44 (1), 2019. doi: 10.14710/jitaa.44.1.10-18
  7. Efficiency of traditional DNA extraction method in PCR detection of porcine DNA in meat mixtures

    Suadi Z.. Jurnal Teknologi, 82 (5), 2020. doi: 10.11113/jt.v82.14636