Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

L. Latifah -  Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-Yogyakarta, Indonesia
D. Maharani -  Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-Yogyakarta, Indonesia
A. Kustantinah -  Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-Yogyakarta, Indonesia
*T. Hartatik -  Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-Yogyakarta, Indonesia
Received: 26 Jun 2018; Published: 11 Dec 2018.
Open Access
Citation Format:
Article Info
Section: Articles
Language: EN
Full Text:
Supp. File(s):
Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat
Subject
Type Other
  Download (116KB)    Indexing metadata
Identification MC4R Gene Polymorphism in Bligon Goat
Subject
Type Other
  Download (1MB)    Indexing metadata
Statistics: 295 222
Abstract

Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat.

Note: This article has supplementary file(s).

Keywords
Bligon; goat; MC4R; SNP; growth traits; association

Article Metrics: