DOI: https://doi.org/10.14710/jitaa.44.1.1-9

Novel variant in the leptin receptor (LEPR) gene and its association with fat quality, odour and flavour in sheep

*A. Gunawan scopus  -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University , Indonesia
F. W. Pramukti  -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University , Indonesia
K. Listyarini  -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University , Indonesia
M. A. Abuzahra  -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University , Indonesia
J. Jakaria scopus  -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University , Indonesia
C. Sumantri scopus  -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University , Indonesia
I. Inounu scopus  -  Center Research for Development of Animal Husbandry, Indonesia
M. J. Uddin scopus  -  School of Veterinary Science, The University of Queensland, Australia
Open Access Copyright (c) 2019 Journal of the Indonesian Tropical Animal Agriculture

Citation Format:
Abstract

The aim of this study was to investigate the association and expression of LEPR with fat quality, flavour and odour in sheep. A total of 47 rams were used in this study. Fatty acids composition, BCFA (Branch Chanin Fatty Acids; MNA, MP, MOA, and EOA) and skatole (MI) representing fat quality, flavour and odour in sheep were measured at ages of 10-12 months old. Identification of novel variant and expression analysis of LEPR gene with different fat quality, odour and flavour were performed by PCR RFLP and qRT-PCR, respectively. A SNP in genomic region g.40854778 A>C of the LEPR gene was associated (P<0.05) with fat quality of saturated fatty acids (SFA) including [tricosanoic acid (C23:0) and tetracosanoic acid (C24:0)] and poly unsaturated fatty acid (PUFA) [docosahexaenoic acid (C22:6n3)]. However, the novel variant of LEPR was not significantly associated (P<0.05) with fat content, odour and flavour (MNA, MP, MOA, EOA, and MI). The mRNA expression analysis showed that LEPR mRNA expression was higher (P<0.01) in sheep found at the AA genotype. It could be concluded that variant SNP in the LEPR may contribute to fatty acid composition without influencing odour and flavour traits in sheep.

Fulltext View|Download
Keywords: flavour; LEPR; mRNA expression; odour; variant
Funding: Ministry of Research and Higher Education of Republic of Indonesia and Ministry of Agriculture

Article Metrics:

Article Info
Section: Articles
Language : EN
Recent articles
Novel variant in the leptin receptor (LEPR) gene and its association with fat quality, odour and flavour in sheep Modelling hatchability and mortality in muscovy ducks using automatic linear modelling and artificial neural network The polymorphism in g.1256G>A of bovine pituitary specific transcription factor-1 (bPIT-1) gene and its association with body weight of Pasundan cattle Front-matter (March 2019) Back-matter (March 2019) More recent articles
Most cited articles
Cholesterol Assimilation of Saccharomyces cerevisiae B-18 isolated from gastrointestinal tract of Javanese duck Market integration of fresh milk in dairy business area of Pamijahan District and Cisarua District, Bogor Regency - Indonesia Effect of dietary mannan-oligosaccharides from copra meal on intestinal microbes and blood profile of broiler chickens THE EFFECT OF FERMENTED OIL PALM FRONDS IN DIET ON BODY WEIGHT GAIN AND MEAT QUALITY OF GOAT Nutritional comparison between dried and ensiled indigofera, papaya and moringa leaves More cited articles

Last update:

  1. Selection Signatures Analysis Reveals Genes Associated with High-Altitude Adaptation in Tibetan Goats from Nagqu, Tibet

    Meilin Jin, Jian Lu, Xiaojuan Fei, Zengkui Lu, Kai Quan, Yongbin Liu, Mingxing Chu, Ran Di, Caihong Wei, Huihua Wang. Animals, 10 (9), 2020. doi: 10.3390/ani10091599

  2. Identification and association of polymorphism in THOC5 gene with fatty acid composition in Indonesian sheep

    K Listyarini, C Sumantri, S Rahayu, M J Uddin, A Gunawan. IOP Conference Series: Earth and Environmental Science, 788 (1), 2021. doi: 10.1088/1755-1315/788/1/012020

  3. Polymorphisms in hormone-sensitive lipase and leptin receptor genes and their association with growth traits in Barki lambs

    Adel H. M. Ibrahim. Veterinary World, 14 (2), 2021. doi: 10.14202/vetworld.2021.515-522

  4. Association and expression study of SEPW1 and JAML as preliminary candidate genes related to lamb odor and flavor

    Mutasem Abuzahra, C. Sumantri, M. J. Uddin, A. Gunawan. Animal Biotechnology, 34 (9), 2023. doi: 10.1080/10495398.2022.2141763

  5. Selection Signature Analyses Revealed Genes Associated With Adaptation, Production, and Reproduction in Selected Goat Breeds in Kenya

    Ruth W. Waineina, Tobias O. Okeno, Evans D. Ilatsia, Kiplangat Ngeno. Frontiers in Genetics, 13 , 2022. doi: 10.3389/fgene.2022.858923

Last update: 2024-11-21 21:43:13

  1. Selection signatures analysis reveals genes associated with high‐altitude adaptation in Tibetan goats from Nagqu, Tibet

    Jin M.. Animals, 10 (9), 2020. doi: 10.3390/ani10091599

  2. Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep

    Munyaneza J.P.. Journal of the Indonesian Tropical Animal Agriculture, 44 (3), 2019. doi: 10.14710/jitaa.44.3.243-251