BibTex Citation Data :
@article{JITAA22900, author = {J. P. Munyaneza and A. Gunawan and R. R. Noor}, title = {Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep}, journal = {Journal of the Indonesian Tropical Animal Agriculture}, volume = {44}, number = {3}, year = {2019}, keywords = {BHMT; Fatty acid; Sheep; SNP}, abstract = { This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphism s on fatty acid traits and cholesterol in lamb s . This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT) , 16 Javanese Thin-Tailed (JTT) , 41 Composite Garut (CG) , 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC) . A total of 61 rams as representative from five breed of sheep were selected for association study . Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method . Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT gen otyping resulted into three genotypes (CC, CT and TT). Gen e frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg E quilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P<0.05) with C14:1, C17:1 and C18:0 fatty acids but not with cholesterol in sheep. SNP g. 9947372 (C>T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids. }, issn = {2460-6278}, pages = {243--251} doi = {10.14710/jitaa.44.3.243-251}, url = {https://ejournal.undip.ac.id/index.php/jitaa/article/view/22900} }
Refworks Citation Data :
This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in lambs. This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT), 16 Javanese Thin-Tailed (JTT), 41 Composite Garut (CG), 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC). A total of 61 rams as representative from five breed of sheep were selected for association study. Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method. Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT genotyping resulted into three genotypes (CC, CT and TT). Gene frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg Equilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P<0.05) with C14:1, C17:1 and C18:0 fatty acids but not with cholesterol in sheep. SNP g. 9947372 (C>T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids.
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