Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination

R.W. Astuti  -  Research Center for Biotechnology Studies, Universitas Gadjah Mada, Indonesia
N. Wijayanti  -  Animal Physiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Indonesia
*A. Haryanto orcid scopus  -  Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia
Received: 16 Oct 2019; Accepted: 15 May 2020; Published: 9 Jun 2020.
DOI: https://doi.org/10.14710/jitaa.45.2.78-90 View
Figure 1 till 6
Subject antibody response; broiler; NDV; recombinant F protein; vaccination,
Type Research Results
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Open Access
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Section: Articles
Language: EN
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Abstract

This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected with pure recombinant F protein compared with treated broilers that were vaccinated with commercial inactive NDV vaccines and control broilers without vaccination. The results showed that the recombinant F protein of NDV was successfully expressed, purified and visualized by SDS-PAGE with Coomassie Brilliant Blue staining and Westernblotting methods as a specific recombinant F protein with a molecular weight of 28 kDa. The pure recombinant F protein then was injected into broilers to determine the antibody response in broiler serum. Indirect ELISA showed that the production of antibodies was high in F protein vaccinated groups in comparison with other treated and control groups. The recombinant F protein has potential to be developed as a recombinant vaccine candidate after truncating the 6x His-tag part to obtain higher antibody respond if compared with antibody production in broiler serum post vaccinated with some commercially available broiler vaccines.

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Keywords: antibody response; broiler; recombinant F protein; NDV; vaccination

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