DOI: https://doi.org/10.14710/jitaa.38.4.239-246

PURIFICATION OF ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDE DERIVED FROM KACANG GOAT MEAT PROTEIN HYDROLYSATE

*J. Jamhari  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
L.M. Yusiati  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
E. Suryanto  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
M.N. Cahyanto  -  Faculty of Agricultural Technology, Gadjah Mada University,, Indonesia
Y. Erwanto  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
M. Muguruma  -  Faculty of Agriculture, University of Miyazaki,, Japan

Citation Format:
Abstract
The objective of this study was to identify the Angiotensin Converting Enzyme (ACE) inhibitorypeptide derived from Kacang goat meat protein hydrolysate. Kacang goat meat loin section washydrolyzed with pepsin, trypsin and chymotrypsin. Protein hydrolysate of Kacang goat meat was thentested the protein concentration and ACE inhibitory activity. ACE inhibitory peptide of the proteinhydrolysate was purified through several steps of purification by column SEP-PAK Plus C18 Cartridgeand RP-HPLC using a Cosmosil column 5PE-SM, 4.6 x 250　mm. The sequence of amino acid of ACEinhibitory peptide was identified by amino acid sequencer. The results showed that amino acidssequence of ACE inhibitory peptide derived from protein hydrolysate of Kacang goat meat was leu-thrglu-ala-pro-leu-asn-pro-lys-ala-arg- asn-glu-lys. It had a molecular weight (MW) of 1581 and occurredat the position of 20th to 33rd residues of b-actin of goat meat protein (Capra hircus). The ACE inhibitoryactivity (IC50) of the peptide was 190 mg/mL or 120 mM.
Fulltext View|Download
Keywords: ACE Inhibitory peptide. meat protein hydrolysate. Kacang goat

Article Metrics:

Article Info
Section: Articles
Language : EN
Recent articles
MUSCLE FIBER DIAMETER AND FAT TISSUE SCORE IN QUAIL (Coturnix-coturnix japonica L) MEAT AS AFFECTED BY DIETARY TURMERIC (Curcuma longa) POWDER AND SWANGI FISH (Priacanthus tayenus) MEAL PERFORMANCE AND DIGESTIVE TRACT DEVELOPMENT OF YOUNG BROILER CHICKENS FED MASH BASAL DIET SUPPLEMENTED WITH RICE HULLS OR WHEAT POLLARD AT DIFFERENT LEVELS THE EFFECT OF FERMENTATION METHOD, MICROBES INOCULATION AND CARBON SOURCE PROPORTION ON THE QUALITY OF ORGANIC FERTILIZER MADE FROM LIQUID WASTES OF CHICKEN SLAUGHTERHOUSE More recent articles
Most cited articles
THE EFFECT OF NON-STARCH POLYSACCHARIDES DERIVED FROM DIFFERENT GRAINS ON PERFORMANCE AND DIGESTIVE ACTIVITY IN LAYING HENS THE USE OF Pomacea canaliculata SNAILS IN FEED TO IMPROVE QUALITY OF ALABIO DUCK (Anas plathyrinchos Borneo) MEAT THE GENETIC DIVERSITY OF KEJOBONG GOAT BASED ON CYTOCHROME B GENE Melanocortin-4 Receptor (MC4R) gene polymorphism and its effect on growth traits in Madura cattle THE INFLUENCE OF USING FISH FERMENTED BY LACTIC ACID BACTERIA AS FEED SUBSTITUTION ON SERUM LIPID PROFILE OF BROILERS More cited articles

Last update:

  1. Increased Inhibition of Angiotensin Converting Enzyme (ACE) Obtained from Indonesian Buffalo Meat Protein Using SEP-PAK Plus C18

    Rio Olympias S, Jamhari ., Edi Suryanto, Rusman ., Setiyono ., Endy Triyannant, Yuny Erwanto, Ragil Yuliatmo. Pakistan Journal of Nutrition, 17 (9), 2018. doi: 10.3923/pjn.2018.434.440

  2. Angiotensin-converting enzyme inhibitor activity of peptides derived from Kacang goat skin collagen through thermolysin hydrolysis

    Arby'in Pratiwi, Thoyib R. Hakim, Mohammad Z. Abidin, Nanung A. Fitriyanto, Jamhari Jamhari, Rusman Rusman, Yuny Erwanto. Veterinary World, 14 (1), 2021. doi: 10.14202/vetworld.2021.161-167

Last update: 2024-11-21 02:57:29

  1. Hydrolyzation of duck meat protein using Bacillus cereus TD5B protease, pepsin, trypsin and their potency as an angiotensin converting enzyme inhibitor

    Winarti A.. Journal of the Indonesian Tropical Animal Agriculture, 44 (3), 2019. doi: 10.14710/jitaa.44.3.266-276

  2. Angiotensin-converting enzyme inhibitor activity of peptides derived from Kacang goat skin collagen through thermolysin hydrolysis

    Arby'in Pratiwi, Thoyib R. Hakim, Mohammad Z. Abidin, Nanung A. Fitriyanto, Jamhari Jamhari, Rusman Rusman, Yuny Erwanto. Veterinary World, 14 (1), 2021. doi: 10.14202/vetworld.2021.161-167

  3. Increased inhibition of angiotensin converting enzyme (ACE) obtained from Indonesian buffalo meat protein using SEP-PAK plus C18

    Sujarwanta R.. Pakistan Journal of Nutrition, 17 (9), 2018. doi: 10.3923/pjn.2018.434.440