DOI: https://doi.org/10.14710/jitaa.35.4.222-226

MOTILITY AND VIABILITY OF FRIESIAN HOLSTEIN SPERMATOZOA IN THREE DIFFERENT EXTENDER STORED AT 5oC

*R.I. Arifiantini  -  Department of Veterinary Clinic, Reproduction and Pathology,, Indonesia
B. Purwantara  -  Department of Veterinary Clinic, Reproduction and Pathology,, Indonesia

Citation Format:
Abstract
The aims of this study was to compare Tris egg yolk and Citrate egg yolk extender andsupplementation of fructose on citrate egg yolk on the quality of Friesian Holstein (FH) bull semenstored at 5 oC. Semen was collected from 5 FH bulls using an artificial vagina. The semen wereevaluated macroscopic and microscopically. The semen divided into three tubes and extended with Trisegg yolk (TEY), Citrate egg yolk (CEY) or Citrate fructose egg yolk (CFEY). Extended semen wasstored at 5 oC and evaluate daily for sperm motility and viability. There was no significant differences(P>0.05) on the sperm viability among three extender, for every time observation during 144 hours ofstorage. This similar finding found on the sperm motility in all extender for 48 hours of storage. Thesperm motility in TEY demonstrated significantly greater (P<0.05) than in CFEY and CEY extender at72 to 120 hours storage. In the end of storage, sperm motility in TEY (35.2 ± 4.1%) and CFEY (33.5 ±2.71%) extender statistically indicated no significant different, and both were greater than CEY. Inconclusion, CFEY support the sperm motility as good as TEY of FH bull.
Fulltext View|Download Email colleagues
Keywords: Citrate. Fructose. FH semen. Tris. citrate

Article Metrics:

Article Info
Section: Articles
Language : EN
Most cited articles
FACTORS INFLUENCING FARMERS’ DECISION TO INCREASE BEEF CATTLE BUSINESS SCALE IN CENTRAL JAVA PROVINCE The effect of hatchery waste meal in ration on nutrient retention and performances of laying quails INFLUENCE OF AGE ON THE SUSCEPTIBILITY OF PIGLETS TO ESCHERICHIA COLI O138:F18 Nitrogen degradation of the limestone-urea mixtures in the rumen of goats Calcium deposition in egg due to substitution of limestone by eggshell flour in feed of laying hens More cited articles

Last update:

  1. Sperm kinematic characteristics of pasundan bulls during frozen semen processing

    Santoso, P I Sitaresmi, D A Mahari, R I Anwar, F B Lupitasaria, Herdis, R I Arifiantini. IOP Conference Series: Earth and Environmental Science, 1341 (1), 2024. doi: 10.1088/1755-1315/1341/1/012005

  2. Addition of bovine serum albumin (BSA) in cauda epididymal plasma-2 (CEP-2) extender to Ongole grade bull sperm motility and membrane integrity during the freezing process

    A Rachmawati, Ismaya, B P Widyobroto, S Bintara, T Susilawati. IOP Conference Series: Earth and Environmental Science, 788 (1), 2021. doi: 10.1088/1755-1315/788/1/012132

  3. Supplementation of Red Dragon Fruit Peel Extract (Hylocereus polyrhizus) in CEP-2 Extender on the Qualities of Limousin Bull Chilled Semen

    S Wahjuningsih, A P A Yekti, A Grace, Amalia. IOP Conference Series: Earth and Environmental Science, 478 (1), 2020. doi: 10.1088/1755-1315/478/1/012084

Last update: 2026-10-26 04:02:08

No citation recorded.