DOI: https://doi.org/10.14710/jkli.77435

Aktivitas Larvasida Eco-Enzyme Berbasis Limbah Kulit Jeruk Siam (Citrus nobilis)

1Program Studi Sarjana Kedokteran, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Warmadewa, Kota Denpasar, Bali 80239, Indonesia

2Departemen Farmakologi, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Warmadewa, Kota Denpasar, Bali 80239, Indonesia

3Departemen Biokimia, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Warmadewa, Kota Denpasar, Bali 80239, Indonesia

4 Program Studi Teknologi Pangan, Fakultas Pertanian Universitas Warmadewa, Kota Denpasar, Bali 80239, Indonesia

View all affiliations
Open Access Copyright 2026 Jurnal Kesehatan Lingkungan Indonesia under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Abstract

Latar belakang: Demam Berdarah Dengue (DBD) termasuk dalam masalah kesehatan kompleks dengan angka kematian yang tinggi. Penatalaksanaan yang adekuat hingga saat ini belum optimal, sehingga modalitas yang tersisa adalah pencegahan. Namun program pencegahan konvensional melalui gerakan 3M+ termasuk penggunaan larvasida konvensional belum sepenuhnya efektif menekan kejadian DBD. Salah satu alternatif yang potensial adalah pemanfaatan larvasida berbahan limbah organik yaitu eco-enzyme. Riset ini memiliki tujuan untuk menganalisis potensi aktivitas larvasida dari eco-enzyme berbasis limbah kulit jeruk siam.

Metode: Metode eksperimental berbasis post-test only controlled group dengan subjek penelitian berupa larva Aedes aegypti instar III yang masih bergerak aktif. Larva instar I dan II, larva mati, serta larva yang sudah berubah menjadi pupa dieksklusi. Uji larvasida dilakukan pada empat kelompok perlakuan dengan konsentrasi eco-enzyme sebesar 1,5%, 3%, 9%, dan 27%, serta satu kelompok kontrol tanpa pemberian eco-enzyme. Analisis dilakukan dengan menghitung jumlah larva yang hidup dan mati. Data dianalisis menggunakan uji Kruskal Walis jika data tidak terdistribusi normal, kemudian dilanjutkan dengan analisis Probit untuk menentukan efektivitas larvasida.

Hasil: Semakin tinggi konsentrasi eco-enzyme limbah kulit jeruk siam, maka angka mortalitas larva Aedes aegypti juga makin tinggi. Konsentrasi tertinggi yang diberikan adalah 27 % dengan hasil kematian total larva Aedes aegypti sebanyak 60 larva (100%). Sebaliknya, pada konsentrasi 1,5%, tidak ditemukan kematian larva (0%). Pada kelompok kontrol tanpa pemberian eco-enzyme juga tidak terdapat kematian larva Aedes aegypti. Uji analisis menemukan bahwa setiap konsentrasi perlakuan terhadap larva Aedes aegypti memiliki efek yang signifikan (P<0,05), dengan nilai LC50 sebesar 6,77 % dan LC99 adalah 13,72 %.

Simpulan:  Eco-enzyme dengan bahan dasar limbah kulit jeruk siam memiliki potensi aktivitas larvasida untuk larva Aedes aegypti.

 

ABSTRACT

Title: Larvicide Activity of Eco-enzyme Derived From Siamese Orange Peel Waste (Citrus nobilis)

Background: Dengue Hemorrhagic Fever (DHF) is a complex health problem with a high mortality rate. Adequate management has not been optimal to date, so the remaining modality is prevention. However, conventional prevention programs through the 3M+ movement, including the use of conventional larvicides, have not been fully effective in suppressing the incidence of dengue fever. One potential alternative is the use of organic waste-based larvicides, namely eco-enzymes. This research aims to analyze the potential larvicidal activity of eco-enzymes made from Siamese orange peel waste.

Method: This study employed an experimental method with a post-test only controlled group design. The research subjects were actively moving Aedes aegypti larvae at the third instar stage. Larvae at instar I and II, dead larvae, and larvae that had developed into pupae were excluded. The larvicidal test was conducted on four treatment groups with eco-enzyme concentrations of 1.5%, 3%, 9%, and 27% as well as one control group without eco-enzyme exposure. Analysis was carried out by counting number of live and dead larvae. Data were analyzed using Kruskal Wallis if not normally distributed, followed by Probit analysis to determine larvicidal effectiveness.

 Result: The higher the concentration of eco-enzyme from Siamese orange peel waste, the higher the mortality rate of Aedes aegypti larvae. The highest concentration administered was 27%, resulting in a total of 60 dead Aedes aegypti larvae (100%). Conversely, at a concentration of 1.5%, no larval deaths were observed (0%). In the control group without eco-enzyme administration, there were also no deaths of Aedes aegypti larvae. The analysis test found that each treatment concentration against Aedes aegypti larvae had a significant effect (P<0.05), with an LC50 value of 6.77% and an LC99 value of 13.72%..

Conclusion: Eco-enzyme derived from Siamese orange peel waste (Citrus nobilis) has potential larvicidal activity against Aedes aegypti larvae.

Note: This article has supplementary file(s).

Fulltext View|Download |  Turnitin
Turnitin
Subject
Type Turnitin
  Download (1MB)    Indexing metadata
 CTA
Copyright Transfer Agreement
Subject
Type CTA
  Download (328KB)    Indexing metadata
 ES
Ethical Statement
Subject
Type ES
  Download (8MB)    Indexing metadata
Keywords: Dengue; Aedes aegypti; Larvasida; Eco-enzyme; Kulit jeruk

Article Metrics:

Article Info
Section: Research Articles
Language : ID
Recent articles
Analisis Faktor Risiko Lingkungan terhadap Kejadian ISPA di Wilayah Lahan Basah Pasca COVID-19: Analisis Multivariat di Palembang Analisis Risiko Kesehatan Lingkungan Pajanan Timbal Pada Air Depo Sumber Air Minum Di Komunitas Identifikasi Kelimpahan Dan Karakteristik Mikroplastik Pada Instalasi Pengolahan Air Limbah Komunal Kota Denpasar More recent articles
Most cited articles
Faktor Risiko Lingkungan Fisik Rumah dan Karakteristik Wilayah Sebagai Determinan Kejadian Penyakit Tuberkulosis Paru di Wilayah Kerja Puskesmas Sentani Kabupaten Jayapura Provinsi Papua Faktor Risiko Bahaya Tempat Kerja dan Lingkungan Rumah terhadap Kesehatan Home-based Worker di Kota Semarang Analisis Risiko Kesehatan dan Kadar Timbal Dalam Darah: (Studi Pada Masyarakat yang Mengkonsumsi Tiram Bakau (Crassostrea gigas) di Sungai Tapak Kecamatan Tugu Kota Semarang) Analisis Faktor Risiko Kejadian Pneumonia Pada Balita Di Wilayah Kerja Puskesmas Sidorejo Kota Pagar Alam Faktor Risiko dan Potensi Penularan Tuberkulosis Paru di Kabupaten Kendal , Jawa Tengah More cited articles
  1. Megawati D, Masyeni S, Yohan B, Lestarini A, Hayati RF, Meutiawati F, et al. Dengue in Bali: Clinical characteristics and genetic diversity of circulating dengue viruses. PLoS Negl Trop Dis. 2017 May 1;11(5). https://doi.org/10.1371/journal.pntd.0005483
  2. Souza-Neto JA, Powell JR, Bonizzoni M. Aedes aegypti vector competence studies: A review. Vol. 67, Infection, Genetics and Evolution. Elsevier B.V.; 2019. p. 191–209. https://doi.org/10.1016/j.meegid.2018.11.009
  3. Permatananda PANK. Dengue Complication in Children. International Journal of Science and Research (IJSR). 2020 Jan 5;9(1):613–9. https://doi.org/10.21275/ART20204067
  4. Kementrian Kesehatan RI. Kasus DBD Meningkat, Kemenkes Galakkan Gerakan 1 Rumah 1 Jumantik (G1R1J) – Sehat Negeriku. Sehat Negeriku Kemenkes RI. 2022;2–3
  5. Direktorat Jenderal Pencegahan dan Pengendalian Penyakit. Membuka Lembaran Baru. LAPORAN TAHUNAN 2022. Demam Berdarah Dengue. Germas. 2022;
  6. Primadi O. Profil Kesehatan Indonesia 2020. kementrian kesehatan republik indonesia. 2021. 480 p
  7. Kemenkes RI. upaya pencegahan DBD dengan 3M Plus. Kementerian Kesehatan RI. 2019;(April):23–4
  8. Permatananda PANK, Cahyawati PN, Pandit IGS, Lestarini A, Aryastuti AASA. Community Empowerment for Vector Control of Dengue Hemorrhagic Fever. Mattawang: Jurnal Pengabdian Masyarakat. 2023 Jun 30;4(2):235–40. https://doi.org/10.35877/454RI.mattawang1871
  9. Istiana. Uji Efektivitas Beberapa Larvasida Terhadap Larva Nyamuk Aedes aegypti dari Banjarmasin Barat. Berkala Kedokteran. 2015;11(1):51–61
  10. Viza RY. Uji Organoleptik Eco-Enzyme dari Limbah Kulit Buah. BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains. 2022 Mar 24;5(1):24–30. https://doi.org/10.31539/bioedusains.v5i1.3387
  11. Lapsia V, Makarand NC. Production, Extraciton and Uses of Eco Enzyme Using Citrus Fruit Waste: Wealth from Waste. Asian Jr of Microbiol Biotech Env Sc. 2020;22(2):346–51
  12. Hariyono H, Madyawati SP, Fauziyah S, Muthu P, Sucipto TH. The Use of Eco-Enzyme as a Larvacide for Aedes aegypti Mosquito Larvae. International Journal of Scientific Research and Management (IJSRM). 2024 Aug 14;12(08):1168–72. https://doi.org/10.18535/ijsrm/v12i08.mp05
  13. Wijaya IMW, Putra IKA. Potensi Daur Ulang Sampah Upacara Adat di Pulau Bali. Jurnal Ecocentrism. 2021;(1):18. https://doi.org/10.36733/jeco.v1i1.1763
  14. Nirmalasari NKDA, Permatananda PANK, Udiyani DPC, Aryastuti AASA, Dewi ES. Aktivitas Antioksidan Ekstrak Limbah Kulit Jeruk Siam Kintamani (Citrus nobilis) dengan Pelarut Polar, Semipolar, dan Nonpolar. Jurnal Ners. 2024;8(1):210–5
  15. World Health Organization. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. Geneva; 2005
  16. Nurhaifah D, Sukesi TW. Efektivitas Air Perasan Kulit Jeruk Manis Sebagai Larvasida Nyamuk Aedes aegypti. Jurnal Kesehatan Masyarakat Nasional. 2015;9(3):207–13. https://doi.org/10.21109/kesmas.v9i3.566
  17. Rachmawati D, Megawati M, Ahmad T. Aktivitas Larvasida Ekstrak Kulit Jeruk Purut (Citrus hystrix D.C) terhadap Larva Nyamuk (Anopheles aconitus). Media Farmasi. 2019;15(2):116. https://doi.org/10.32382/mf.v15i2.1074
  18. Permatananda PANK, Pandit IGS, Aryastuti AASA, Cahyawati PN, Lestarini A. Acute Toxicity Test of Orange Peel Waste (Citrus nobilis) Based Eco-enzyme on Tilapia Fish (Oreochromis niloticus). Bali Medical Journal. 2024;13(2):59–62
  19. Irianto IDK, Purnomo K, Amanati A, Savila D, Mardiyaningsih A. Aktivitas antibakteri Eco-enzyme Limbah Citrus sinensis, musa paradisiaca L. var Bluggoe, dan kombinasinya terhadap Staphylococcus aureus. Majalah Farmaseutik. 2023;19(4):504–13
  20. Suputra IPGJ, Permatananda PANK, Dewi NWES, Aryastuti AASA. The Potential of Tulsi Ethanol (Ocimum tenuiflorum) Extract as a Natural Larvicide on Aedes aegypti Larvae. African Journal of Biological Science. 2024;6(13):886–92
  21. Permatananda PANK, Pandit IGS. Characteristic of Orange Peel Waste-Based on Eco Enzyme at Different Fermentation Duration. Jurnal Penelitian Pendidikan IPA. 2023 Jun 25;9(6):4289–93. https://doi.org/10.29303/jppipa.v9i6.3527
  22. Rasit N, Fern LH, Ghani AWAK. Production and Characterization of Eco Enzyme Produced From Tomato and Orange Wastes and Its Influence On The Aquaculture Sludge. International Journal of Civil Engineering and Technology. 2019;10(03):967–80
  23. Samriti, Sarabhai S, Arya A. Garbage enzyme: A study on compositional analysis of kitchen waste ferments. The Pharma Innovation Journal. 2019;8(4):1193–7
  24. Putri AAAR, Permatananda PANK, Pandit IGS, Lestarini A. Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration. Acta Biochimica Indonesiana. 2024 Dec 27;7(2):173. https://doi.org/10.32889/actabioina.173
  25. Dwiyanti F, Kurniawan B, Lisiswanti R, Mutiara H. Hubungan pH Air terhadap Pertumbuhan Jentik Nyamuk Aedes aegypti. Medula. 2023;13(3):158–63

Last update:

No citation recorded.

Last update: 2026-01-29 20:20:44

No citation recorded.