DOI: https://doi.org/10.14710/jkli.78901

Factors Associated with Airborne Microbial Counts in the Inpatient Installation of Hospital X, Semarang City

Department of Public Health, Universitas Diponegoro, Jl. Prof. Jacub Rais, Tembalang, Semarang, Indonesia 50275, Indonesia

Open Access Copyright 2026 Jurnal Kesehatan Lingkungan Indonesia under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

Background: Healthcare-associated infections (HAIs) remain a challenge in healthcare services, with a prevalence of approximately 5–15% in developed countries and higher rates in developing countries. In Indonesia, the prevalence is estimated at 10–15% among hospitalized patients. Although air quality in the Inpatient Installation of Hospital X met the established standards based on routine monitoring in 2024, the measurements did not include patient visiting hours, which may increase microbial contamination in the air. This study aimed to analyze the factors associated with airborne microbial counts in the Inpatient Installation of Hospital X, Semarang.

Methods: An analytical observational study with a cross-sectional design was conducted in 35 Class III inpatient wards selected as the study sample. Data analysis included univariate analysis, bivariate analysis using the chi-square test, and multivariate analysis using the Backward Wald logistic regression method.

Results: The mean room temperature was 26.5°C, relative humidity was 67.8%, light intensity was 218 lux, and the mean airborne microbial count was 304.5 CFU/m³. Bivariate analysis showed that temperature (p=0.001), relative humidity (p=0.030), number of patients (p=0.044), air circulation system (p=0.013), and personal hygiene (p=0.022) were significantly associated with airborne microbial counts. Multivariate analysis identified relative humidity (p=0.028) and personal hygiene (p=0.024) as the most dominant factors affecting airborne microbial counts.

Conclusion: The findings confirm that temperature, relative humidity, number of patients, air circulation system, and personal hygiene affect airborne microbial counts in the Class III Inpatient Installation of Hospital X, with relative humidity and personal hygiene exerting the strongest influence.

 

 

ABSTRACT

Title: Factors Associated with Airborne Microbial Counts in the Inpatient Installation of Hospital X, Semarang City

Background: Healthcare-associated infections (HAIs) remain a challenge in healthcare services, with a prevalence of approximately 5–15% in developed countries and even higher rates in developing countries. In Indonesia, the prevalence is estimated to range from 10% to 15% among hospitalized patients. Although routine monitoring conducted in 2024 indicated that the air quality in the Class III Inpatient Installation of Hospital X met the established standards, the measurements did not include patient visiting hours, during which increased human activity may elevate airborne microbial contamination. This study aimed to analyze the factors associated with airborne bacterial counts in the Class III Inpatient Installation of Hospital X, Semarang.

Methods: An analytical observational study with a cross-sectional design was conducted in 35 Class III inpatient wards selected as the study sample. Data were analyzed using univariate analysis, bivariate analysis with the chi-square test, and multivariate analysis using the Backward Wald logistic regression method.

Results: The mean room temperature was 26.5°C, relative humidity was 67.8%, light intensity was 218 lux, and the mean airborne bacterial count was 304.5 CFU/m³. Bivariate analysis showed that temperature (p=0.001), relative humidity (p=0.030), number of patients (p=0.044), air circulation system (p=0.013), and personal hygiene (p=0.022) were significantly associated with airborne bacterial counts. Multivariate analysis identified relative humidity (p=0.028) and personal hygiene (p=0.024) as the most influential factors affecting airborne bacterial counts.

Conclusion: Temperature, relative humidity, number of patients, air circulation system, and personal hygiene significantly influenced airborne bacterial counts in the Class III Inpatient Installation of Hospital X, with relative humidity and personal hygiene being the most dominant factors.

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Keywords: Physical Air Quality; Airborne Microorganisms; Nosocomial Infections; Personal Hygiene; Hospital Sanitation

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Language : ID
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  2. Vilches TN, Bonesso MF, Guerra HM, Fortaleza CMCB, Park AW, Ferreira CP. The role of intra and inter-hospital patient transfer in the dissemination of heathcare-associated multidrug-resistant pathogens. Epidemics. 2019;26(November 2018):104–15. https://doi.org/10.1016/j.epidem.2018.11.001
  3. Pangaribuan R, Patungo V, Sudarman. TINGKAT KEPATUHAN PERAWAT DALAM IMPLEMENTASI FIVE MOMENTS CUCI TANGAN DI RSUD YOWARI KABUPATEN JAYAPURA. Sentani Nurs J. 2019;54–61. https://ejournal.unijaya.ac.id/index.php/snj/article/view/45
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  5. Rompas CL, Pinontoan O, Maddusa S, Kesehatan F, Universitas M, Ratulangi S. PEMERIKSAAN ANGKA KUMAN UDARA DI RUANG RAWAT INAP RUMAH SAKIT UMUM GMIM PANCARAN KASIH MANADO. Vol. 8, Jurnal KESMAS. 2019
  6. Indonesia MKR. Keputusan Menteri Kesehatan Republik Indonesia Nomor 1204/Menkes/SK/X/2004 Tentang Persyaratan Kesehatan Lingkungan Rumah Sakit. 2004 p. 1–61
  7. Indonesia KK. PERATURAN MENTERI KESEHATAN REPUBLIK INDONESIA NOMOR 2 TAHUN 2023 TENTANG PERATURAN PELAKSANAAN PERATURAN PEMERINTAH NOMOR 66 TAHUN 2014 TENTANG KESEHATAN LINGKUNGAN. 2023 p. 1–179
  8. Ginting DB, Santosa I, Indra Trigunarso S, Studi III Kesehatan Lingkungan Politeknik Kesehatan Tanjungkarang PD, Studi IV Kesehatan Lingkungan Politeknik Kesehatan Tanjungkarang PD. Pengaruh Suhu, Kelembaban Dan Kecepatan Angin Air Conditioner (AC) Terhadap Jumlah Angka Kuman Udara Ruangan. Vol. 11, Jurnal Analis Kesehatan. 2022
  9. Santosa I, Trigunarso SI, Udara AK. Pengaruh Suhu , Kelembaban Dan Kecepatan Angin Air Conditioner ( AC ) Terhadap Jumlah Angka Kuman Udara Ruangan The Effect of Temperature , Humidity and Wind Speed of Air Conditioner ( AC ) on Total Germ Numbers of Room Air. J Anal Kesehat. 2022;11(1):44–50
  10. Yonata Q, Thohari I, Marlik. Faktor yang Berhubungan dengan Angka Kuman Udara di Rumah Sakit Soemitro Surabaya. J Penelit Kesehat Suara Forikes. 2020;11(3):264–6
  11. Ahlawat A, Wiedensohler A, Mishra SK. An Overview on the Role of Relative Humidity in Airborne Transmission of SARS-CoV-2 in Indoor Environments. Aerosol Air Qual Res. 2020;2(V):1856–61
  12. Bozic A, Kandu M. Relative humidity in droplet and airborne transmission of disease. J Biol Phys. 2020;
  13. Marr LC, Tang JW, Mullekom J Van, Lakdawala SS. Mechanistic insights into the effect of humidity on airborne influenza virus survival , transmission and incidence. INTERFACE. 2026;16(June):20180298
  14. Marco- WH, King F, Shuweihdi F, Fletcher LA, Dancer SJ, Noakes CJ. What is the relationship between indoor air quality parameters and airborne microorganisms in hospital environments ? A systematic review and meta- ­ analysis. WILEY. 2021;31(April):1308–22
  15. Kusumawardhani C, Gunawan AT, Cahyono T. FAKTOR LINGKUNGAN FISIK YANG BERHUBUNGAN DENGAN ANGKA KUMAN UDARA DI RUANG RAWAT INAP KELAS I, II, DAN III RST WIJAYAKUSUMA PURWOKERTO TAHUN 2018. Keslingmas. 2018;38(2):124–243
  16. Wulandari W, Sutomo AH, Iravati S, Kedokteran F, Mada UG, Kedokteran F, et al. ANGKA KUMAN UDARA DAN LANTAI RUANG RAWAT INAP RUMAH SAKIT PKU MUHAMMADIYAH YOGYAKARTA. J Berk Kesehat. 2015;1(1):13–20
  17. Yu Y, Yin S, Kuan Y, Xu Y, Gao X. Characteristics of airborne micro-organisms in a neurological intensive care unit : Results from China. J Int Med Res. 2015; https://doi.org/10.1177/0300060514562055
  18. Agus Nugroho D, Budiyono, Nurjazuli. FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN ANGKA KUMAN UDARA DI RUANG RAWAT INAP KELAS III RSUD DR. MOEWARDI SURAKARTA. J Kesehat Masy. 2016;4(4):2356–3346. http://ejournal-s1.undip.ac.id/index.php/jkm
  19. Tsay MD, Tseng CC, Wu NX, Lai CY. Size distribution and antibiotic-resistant characteristics of bacterial bioaerosol in intensive care unit before and during visits to patients. Environ Int. 2020 Nov;144. https://doi.org/10.1016/j.envint.2020.106024
  20. Rn KT, Ms PL, Carenza A, Baumgarten K, Ann B, Ms L, et al. The effects of a novel, continuous disinfectant technology on methicillin-resistant Staphylococcus aureus (MRSA), fungi, and aerobic bacteria in 2 separate intensive care units in 2 different states_ An experimental design with observed impact on health care associated infections (HAIs). AJIC Am J Infect Control. 2024;52(8):884–92. https://doi.org/10.1016/j.ajic.2024.03.013
  21. Vonci N, Marco MF De, Grasso A, Spataro G, Cevenini G, Messina G. Association between air changes and airborne microbial contamination in operating rooms. J Infect Public Health. 2019;12(6):827–30
  22. Kubde D, Badge AK, Ugemuge S, Shahu S. Importance of Hospital Infection Control. Cureus. 2023;15(12):1–9. . https://doi.org/10.1016/j.jiph.2019.05.010
  23. Kokubo M, Ogura D, Nakajima M, Maruyama F, Fujiyoshi S, Noda J. Indoor temperature , humidity , and traditional and modern houses in Japan microorganisms in. E3S Web Conf. 2023;01054:1–5
  24. Onmek N, Kongcharoen J, Singtong A, Penjumrus A, Junnoo S. Environmental Factors and Ventilation Affect Concentrations of Microorganisms in Hospital Wards of Southern Thailand. 2020;2020
  25. Ojanperä H, Kanste I, Syrjala H. Hand-hygiene compliance by hospital staff and incidence of health- care-associated infections , Finland. Bull World Heal Organ. 2020;(April):475–83

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