Bui Xuan Thanh, Quyen Vo Thi Kim, Phuc Luu Vinh, Tin Nguyen Thanh, Hien Vo Thi Dieu, Thanh Cao Ngoc Dan, Quoc Tuc Dinh



Antibiotic residues in the environment and their potential toxic effects have been considered as one of the emerging research area in the environmental field. Their continuous introduction in our environment may increase their negative impacts on human health.  In this study, the eliminations of antibiotic such as Norfloxacin (NOR), Ciprofloxacin (CIP), Ofloxacin (OFL) and Sulfamethoxazole (SMZ) in wastewater of hospital were processed by membrane bioreactor (MBR) coupled with ozonation process. In particular, the MBR was applied for the antibiotic removals followed by ozonation process as a post-treatment stage to create an adequate integration to enhance removal efficiency. Achieved results after MBR treatment showed that the removal efficiency of NOR, CIP, OFL and SMZ were 90 ± 4.0% , 83 ± 13% , 81 ± 13 % and  39 ± 6%, respectivley. In addition, those antibiotic matters were continously removed by ozonation process with the removal efficiency of 87±9.0% , 83±1.0% , 81±2.3% and 66±2.3% for NOR, CIP, OFL and SMZ, respectively. In summary, antibiotics could be basically limited by the combination of MBR and ozonation before discharging in aquatic environment.


antibiotic, membrane bioreactor, ozonation, hospital, wastewater

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Alexy, R., Sommer, A., Lange, F.T., Kuemmerer, K. 2006. Local use of antibiotics and their input and fate in a small sewage treatment plant–significance of balancing and analysis on a local scale vs. nationwide scale. Acta hydrochimica et hydrobiologica, 34(6), 587-592.

Andreozzi, R., Canterino, M., Marotta, R., Paxeus, N. 2005. Antibiotic removal from wastewaters: the ozonation of amoxicillin. Journal of hazardous Materials, 122(3), 243-250.

Andreozzi, R., Marotta, R., Pinto, G., Pollio, A. 2002. Carbamazepine in water: persistence in the environment, ozonation treatment and preliminary assessment on algal toxicity. Water Research, 36(11), 2869-2877.

Apha, A. 1998. WEF (1998) Standard methods for the examination of water and wastewater, American Public Health Association, Washington, DC.

Baig, S., Liechti, P. 2001. Ozone treatment for biorefractory COD removal. Water Science and Technology, 43(2), 197-204.

Balcıoğlu, I.A., Ötker, M. 2003. Treatment of pharmaceutical wastewater containing antibiotics by O 3 and O 3/H 2 O 2 processes. Chemosphere, 50(1), 85-95.

Batt, A.L., Kim, S., Aga, D.S. 2007. Comparison of the occurrence of antibiotics in four full-scale wastewater treatment plants with varying designs and operations. Chemosphere, 68(3), 428-435.

Carey, J. 1992. Introduction to advanced oxidation processes(AOP) for destruction of organics in wastewater. Water Quality Research Journal of Canada, 27(1), 1-21.

Christian, T., Schneider, R.J., Färber, H.A., Skutlarek, D., Meyer, M.T., Goldbach, H.E. 2003. Determination of antibiotic residues in manure, soil, and surface waters. Acta hydrochimica et hydrobiologica, 31(1), 36-44.

Dantas, R.F., Contreras, S., Sans, C., Esplugas, S. 2008.

Sulfamethoxazole abatement by means of ozonation. Journal of Hazardous Materials, 150(3), 790-794.

Dinh, Q.T., Alliot, F., Moreau-Guigon, E., Eurin, J., Chevreuil, M., Labadie, P. 2011. Measurement of trace levels of antibiotics in river water using on-line enrichment and triple-quadrupole LC–MS/MS. Talanta, 85(3), 1238-1245.

Duong, H.A., Pham, N.H., Nguyen, H.T., Hoang, T.T., Pham, H.V., Pham, V.C., Berg, M., Giger, W., Alder, A.C. 2008. Occurrence, fate and antibiotic resistance of fluoroquinolone antibacterials in hospital wastewaters in Hanoi, Vietnam. Chemosphere, 72(6), 968-973.

Fent, K., Weston, A.A., Caminada, D. 2006. Ecotoxicology of human pharmaceuticals. Aquatic toxicology, 76(2), 122-159.

Göbel, A., Thomsen, A., McArdell, C.S., Joss, A., Giger, W. 2005. Occurrence and sorption behavior of sulfonamides, macrolides, and trimethoprim in activated sludge treatment. Environmental science & technology, 39(11), 3981-3989.

Golet, E.M., Xifra, I., Siegrist, H., Alder, A.C., Giger, W. 2003. Environmental exposure assessment of fluoroquinolone antibacterial agents from sewage to soil. Environmental science & technology, 37(15), 3243-3249.

Halling-Sørensen, B. 2000. Algal toxicity of antibacterial agents used in intensive farming. Chemosphere, 40(7), 731-739.

Hua, W., Bennett, E.R., Letcher, R.J. 2006. Ozone treatment and the depletion of detectable pharmaceuticals and atrazine herbicide in drinking water sourced from the upper Detroit River, Ontario, Canada. Water Research, 40(12), 2259-2266.

Huber, M.M., GÖbel, A., Joss, A., Hermann, N., LÖffler, D., McArdell, C.S., Ried, A., Siegrist, H., Ternes, T.A., von Gunten, U. 2005. Oxidation of pharmaceuticals during ozonation of municipal wastewater effluents: a pilot study. Environmental science & technology, 39(11), 4290-4299.

Joss, A., Zabczynski, S., Göbel, A., Hoffmann, B., Löffler, D., McArdell, C.S., Ternes, T.A., Thomsen, A., Siegrist, H. 2006. Biological degradation of pharmaceuticals in municipal wastewater treatment: proposing a classification scheme. Water research, 40(8), 1686-1696.

Kolpin, D.W., Furlong, E.T., Meyer, M.T., Thurman, E.M., Zaugg, S.D., Barber, L.B., Buxton, H.T. 2002. Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999-2000: A national reconnaissance. Environmental science & technology, 36(6), 1202-1211.

Kümmerer, K., Al-Ahmad, A., Mersch-Sundermann, V. 2000.

Biodegradability of some antibiotics, elimination of the genotoxicity and affection of wastewater bacteria in a simple test. Chemosphere, 40(7), 701-710.

Lanzky, P., Halting-Sørensen, B. 1997. The toxic effect of the antibiotic metronidazole on aquatic organisms. Chemosphere, 35(11), 2553-2561.

Larsen, T.A., Lienert, J., Joss, A., Siegrist, H. 2004. How to avoid pharmaceuticals in the aquatic environment. Journal of Biotechnology, 113(1), 295-304.

Li, B., Zhang, T. 2010. Biodegradation and adsorption of antibiotics in the activated sludge process. Environmental science & technology, 44(9), 3468-3473.

Li, C., Cabassud, C., Guigui, C. 2015. Evaluation of membrane bioreactor on removal of pharmaceutical micropollutants: a review. Desalination and Water Treatment, 55(4), 845-858.

Liu, P., Zhang, H., Feng, Y., Yang, F., Zhang, J. 2014. Removal of trace antibiotics from wastewater: a systematic study of nanofiltration combined with ozone-based advanced oxidation processes. Chemical Engineering Journal, 240, 211-220.

Oaks, J.L., Gilbert, M., Virani, M.Z., Watson, R.T., Meteyer, C.U., Rideout, B.A., Shivaprasad, H., Ahmed, S., Chaudhry, M.J.I., Arshad, M. 2004. Diclofenac residues as the cause of vulture population decline in Pakistan. Nature, 427(6975), 630-633.

Pomiès, M., Choubert, J.-M., Wisniewski, C., Coquery, M. 2013. Modelling of micropollutant removal in biological wastewater treatments: a review. Science of the Total Environment, 443, 733-748.

Radjenović, J., Petrović, M., Barceló, D. 2009. Fate and distribution of pharmaceuticals in wastewater and sewage sludge of the conventional activated sludge (CAS) and advanced membrane bioreactor (MBR) treatment. Water research, 43(3), 831-841.

Sacher, F., Gabriel, S., Metzinger, M., Stretz, A., Wenz, M., Lange, F.T., BRAUCH, H.-J., BLANKENHOM, I. 2002. Arzneimittelwirkstoffe im Grundwasser: Ergebnisse eines Monitoring-Programms in Baden-Württemberg. Vom Wasser, 99, 183-195.

Sipma, J., Osuna, B., Collado, N., Monclús, H., Ferrero, G., Comas, J., Rodriguez-Roda, I. 2010. Comparison of removal of pharmaceuticals in MBR and activated sludge systems. Desalination, 250(2), 653-659.

Ternes, T.A., Joss, A., Siegrist, H. 2004. Peer reviewed: scrutinizing pharmaceuticals and personal care products in wastewater treatment. Environmental Science & Technology, 38(20), 392A-399A.

Vasconcelos, T.G., Kümmerer, K., Henriques, D.M., Martins, A.F. 2009. Ciprofloxacin in hospital effluent: degradation by ozone and photoprocesses. Journal of hazardous Materials, 169(1), 1154-1158.

Verlicchi, P., Al Aukidy, M., Zambello, E. 2012. Occurrence of pharmaceutical compounds in urban wastewater: removal, mass load and environmental risk after a secondary treatment—a review. Science of the total environment, 429, 123-155.

Visvanathan, C., Aim, R.B., Parameshwaran, K. 2000. Membrane separation bioreactors for wastewater treatment. Critical Reviews in Environmental Science and Technology, 30(1), 1-48.

Yamashita, N., Yasojima, M., Nakada, N., Miyajima, K., Komori, K., Suzuki, Y., Tanaka, H. 2006. Effects of antibacterial agents, levofloxacin and clarithromycin, on aquatic organisms. Water science and technology, 53(11), 65-72.

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