PEMODELAN MATEMATIKA PROSES PRODUKSI POLYHYDROXYBUTYRATE (PHB) DARI TAPIOKA

Margono Margono; Rochmadi Rochmadi; Siti Syamsiah; Muhamad Nurcahyanto

doi:10.14710/reaktor.13.4.211-219

DOI: https://doi.org/10.14710/reaktor.13.4.211-219

PEMODELAN MATEMATIKA PROSES PRODUKSI POLYHYDROXYBUTYRATE (PHB) DARI TAPIOKA

*Margono Margono - Jurusan Teknik Kimia, Fakultas Teknik, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, Indonesia

Rochmadi Rochmadi - Jurusan Teknik Kimia, Fakultas Teknik, Universitas Gadjah Mada, Yogyakarta, Indonesia

Siti Syamsiah - Jurusan Teknik Kimia, Fakultas Teknik, Universitas Gadjah Mada, Yogyakarta, Indonesia

Muhamad Nurcahyanto - Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Yogyakarta, Indonesia

Published: 18 Dec 2011.

BibTex Citation Data :

@article{Reaktor3585,
    author = {Margono Margono and Rochmadi Rochmadi and Siti Syamsiah and Muhamad Nurcahyanto},
    title = {PEMODELAN MATEMATIKA PROSES PRODUKSI POLYHYDROXYBUTYRATE (PHB) DARI TAPIOKA},
    journal = {Reaktor},
  volume = {13},
    number = {4},
    year = {2011},
    keywords = {Bacillus cereus IFO 13690; model matematika; polyhydroxybutyrate; simulasi},
    abstract = {  MATHEMATICS MODELLING OF POLYHYDROXYBUTYRATE (PHB) PRODUCTION FROM TAPIOCA.   One of the technical approaches to get improvement of PHB productivity was a process simulation by using mathematical modeling. The objective of this research was to develop mathematical model which could be used to simulate fermentation of polyhydroxybutyrate production by Bacillus cereus IFO 13690 using tapioca substrates. Three different experiments with initial ammonium of 0.286, 0.566, and 1.203 g/L were carried out in 5 L fermentor and 500 rpm of agitation speed. The pH medium was controlled at 5.6 after it came down from the initial pH of 6.8. Meanwhile, the initial doT was 70% air saturation and also came down to and maintained at doT of 5% air saturation. PHB accumulation was growth associated product. Model of bacteria mechanism on utilizing tapioca and the mathematical model were proposed. The proposed model was suitable with the experimental phenomena. However, the rate of fermentation process was the controlling rate for overall PHB synthesis.             Abstrak         Salah satu pendekatan teknis untuk melakukan optimasi proses fermentasi adalah melakukan simulasi menggunakan model matematika.    Tujuan penelitian ini adalah mengembangkan model matematika yang dapat digunakan untuk melakukan simulasi proses pada fermentasi produksi polyhydroxybutyrate (PHB) dari tapioka oleh Bacillus cereus IFO 13690. Tiga buah percobaan dengan konsentrasi amonium awal 0,286, 0,566, dan 1,203 g/L dilakukan pada fermentor dengan volume 5 L dan kecepatan pengadukan 500 rpm. Percobaan dilakukan dengan kondisi awal  pH 6,8 dan konsentrasi oksigen terlarut (doT) 70% udara jenuh.   Kondisi awal tidak dikontrol sehingga pH medium turun dan dijaga pada 5,6 menggunakan pengontrol pH.   Sedangkan doT turun dan dikontrol pada 5% udara jenuh.   Data percobaan dan model matematika menunjukkan bahwa produksi PHB mengikuti pola pertumbuhan berasosiasi dengan produk (growth associated product). Model mekanisme pemanfaatan tapioka dan model matematika yang diusulkan sesuai dengan fenomena proses yang terjadi dalam percobaan. Kecepatan proses fermentasi merupakan kecepatan yang mengontrol keseluruhan proses sintesis PHB.  },
   issn = {2407-5973},   pages = {211--219}  doi = {10.14710/reaktor.13.4.211-219},
    url = {https://ejournal.undip.ac.id/index.php/reaktor/article/view/3585}
}

Citation Format:

Abstract

MATHEMATICS MODELLING OF POLYHYDROXYBUTYRATE (PHB) PRODUCTION FROM TAPIOCA. One of the technical approaches to get improvement of PHB productivity was a process simulation by using mathematical modeling. The objective of this research was to develop mathematical model which could be used to simulate fermentation of polyhydroxybutyrate production by Bacillus cereus IFO 13690 using tapioca substrates. Three different experiments with initial ammonium of 0.286, 0.566, and 1.203 g/L were carried out in 5 L fermentor and 500 rpm of agitation speed. The pH medium was controlled at 5.6 after it came down from the initial pH of 6.8. Meanwhile, the initial doT was 70% air saturation and also came down to and maintained at doT of 5% air saturation. PHB accumulation was growth associated product. Model of bacteria mechanism on utilizing tapioca and the mathematical model were proposed. The proposed model was suitable with the experimental phenomena. However, the rate of fermentation process was the controlling rate for overall PHB synthesis.

Abstrak

Salah satu pendekatan teknis untuk melakukan optimasi proses fermentasi adalah melakukan simulasi menggunakan model matematika. Tujuan penelitian ini adalah mengembangkan model matematika yang dapat digunakan untuk melakukan simulasi proses pada fermentasi produksi polyhydroxybutyrate (PHB) dari tapioka oleh Bacillus cereus IFO 13690. Tiga buah percobaan dengan konsentrasi amonium awal 0,286, 0,566, dan 1,203 g/L dilakukan pada fermentor dengan volume 5 L dan kecepatan pengadukan 500 rpm. Percobaan dilakukan dengan kondisi awal pH 6,8 dan konsentrasi oksigen terlarut (doT) 70% udara jenuh. Kondisi awal tidak dikontrol sehingga pH medium turun dan dijaga pada 5,6 menggunakan pengontrol pH. Sedangkan doT turun dan dikontrol pada 5% udara jenuh. Data percobaan dan model matematika menunjukkan bahwa produksi PHB mengikuti pola pertumbuhan berasosiasi dengan produk (growth associated product). Model mekanisme pemanfaatan tapioka dan model matematika yang diusulkan sesuai dengan fenomena proses yang terjadi dalam percobaan. Kecepatan proses fermentasi merupakan kecepatan yang mengontrol keseluruhan proses sintesis PHB.

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Keywords: Bacillus cereus IFO 13690; model matematika; polyhydroxybutyrate; simulasi

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Section: Research Article

Language : EN

In Volume 13, Nomor 4, Desember 2011

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