Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk

Rudy Agustriyanto; Akbarningrum Fatmawati; Yusnita Liasari

doi:10.9767/bcrec.7.2.4046.137-141

Coconut husk is classified as complex lignocellulosic material that contains cellulose, hemicellulose, lignin, and some other extractive compounds. Cellulose from coconut husk can be used as fermentation substrate after enzymatic hydrolysis. In contrary, lignin content from the coconut husk will act as an inhibitor in this hydrolysis process. Therefore, a pretreatment process is needed to enhance the hydrolysis of cellulose. The objective of this research is to investigate the production of the glucose through dilute acid pretreatment and to obtain its optimum operating conditions. In this study, the pretreatment was done using dilute sulfuric acid in an autoclave reactor. The pretreatment condition were varied at 80°C, 100°C, 120°C and 0.9%, 1.2%, 1.5% for temperature and acid concentration respectively. The acid pretreated coconut husk was then hydrolyzed using commercial cellulase (celluclast) and β-glucosidase (Novozyme 188). The hydrolysis time was 72 hours and the operating conditions were varied at several temperature and pH. From the experimental results it can be concluded that the delignification temperature variation has greater influence than the acid concentration. The optimum operating condition was obtained at pH 4 and 50°C which was pretreated at 100°C using 1.5% acid concentration. Copyright © 2012 by BCREC UNDIP. All rights reserved. (Selected Paper from International Conference on Chemical and Material Engineering (ICCME) 2012)

Received: 28th September 2012, Revised: 2nd October 2012, Accepted: 4th October 2012

[How to Cite: R. Agustriyanto, A. Fatmawati, Y. Liasari. (2012). Study of Enzymatic Hydrolysis of Dilute Acid Pretreated Coconut Husk. Bulletin of Chemical Reaction Engineering & Catalysis, 7(2): 137-141. doi:10.9767/bcrec.7.2.4046.137-141]

[How to Link / DOI: http://dx.doi.org/10.9767/bcrec.7.2.4046.137-141 ]

| View in

Keywords

Coconut; Enzyme; Hydrolysis; Lignocellulose

Copyright Transfer Agreement

The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Bulletin of Chemical Reaction Engineering & Catalysis journal and Department of Chemical Engineering Diponegoro University as publisher of the journal.

Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as: electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Bulletin of Chemical Reaction Engineering and Catalysis journal and Department of Chemical Engineering Diponegoro University.

Bulletin of Chemical Reaction Engineering & Catalysis journal and Department of Chemical Engineering Diponegoro University, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Bulletin of Chemical Reaction Engineering & Catalysis (BCREC) are sole and exclusive responsibility of their respective authors and advertisers.

The Copyright Transfer Agreement Form can be downloaded here: [Copyright Transfer Form BCREC 2016]
The copyright form should be signed originally and send to the Editorial Office in the form of original mail, scanned document or fax :

Assoc. Prof. Dr. I. Istadi (Editor-in-Chief)
Editorial Office of Bulletin of Chemical Reaction Engineering & Catalysis
Department of Chemical Engineering, Diponegoro University
Jl. Prof. Soedarto, Kampus Undip Tembalang, Semarang, Central Java, Indonesia 50275
Telp.: +62-24-7460058, Fax.: +62-24-76480675
E-mail: bcrec@live.undip.ac.id